kn-62 and bicuculline-methiodide

Whole cell patch-clamp recordings from GABAergic cells of thalamic reticular nucleus (RTN) in thalamocortical slices made from postnatal day 6 (P6) to 10 (P10) were used to investigate the pattern of rebound bursts (RBs) triggered by an injection of hyperpolarizing current into RTN cells. The number of RBs in the RTN and the overlying Na(+)/K(+) spikes changed in an age-dependent manner. The generation of RBs depended largely on the amplitude of the after-hyperpolarizations (AHPs). RB patterns in response to hyperpolarizing current injection into relay cells were markedly different from RB patterns in RTN cells with an after-depolarization. GABA(A) receptor antagonist bicuculline methiodide (BMI) changed burst firing patterns, increasing the duration of RB and decreasing the amplitude of AHP in RTN cells. Furthermore, local puffs of NMDA in the presence of BMI induced RBs. K(+) channel blocker 4-aminopyridine partially mimicked the effect of BMI on AHPs. The shapes of RBs were altered by a selective CaMKII inhibitor KN-62, but not by an inactive analog KN-04.

Topics: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; 4-Aminopyridine; Action Potentials; Animals; Animals, Newborn; Bicuculline; Calcium-Calmodulin-Dependent Protein Kinase Type 2; GABA Antagonists; GABA-A Receptor Antagonists; gamma-Aminobutyric Acid; Mice; Mice, Inbred ICR; Neural Inhibition; Neurons; Organ Culture Techniques; Potassium Channel Blockers; Protein Kinase Inhibitors; Receptors, GABA-A; Thalamic Nuclei