ketodihydrosphingosine has been researched along with thermozymocidin* in 1 studies
1 other study(ies) available for ketodihydrosphingosine and thermozymocidin
Article | Year |
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Quantification of 3-ketodihydrosphingosine using HPLC-ESI-MS/MS to study SPT activity in yeast
Serine palmitoyltransferase (SPT) catalyzes the rate-limiting step of condensation of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine (3KDS). Here, we report a HPLC-ESI-MS/MS method to directly quantify 3KDS generated by SPT. With this technique, we were able to detect 3KDS at a level comparable to that of dihydrosphingosine in yeast Topics: Chromatography, High Pressure Liquid; Fatty Acids, Monounsaturated; Saccharomyces cerevisiae; Serine C-Palmitoyltransferase; Spectrometry, Mass, Electrospray Ionization; Sphingosine; Tandem Mass Spectrometry | 2018 |