kaempferol-3-o-galactoside and quinone

Calluna vulgaris L. (Ericaceae) is used for the treatment of various inflammatory ailments in traditional medicines. In order to evaluate this ethnobotanical information, its anti-inflammatory and antinociceptive activities were studied using in vivo experimental models in mice. The ethanolic extract of the plant was first fractionated into five extracts; namely, n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol, and water fractions. Among them, the EtOAc Fr. was found to be the most effective and was further subjected to bioassay-guided fractionation and isolation procedures. After successive column chromatography applications, on Sephadex LH-20 and silica gel, a component, which is responsible for the above-mentioned activities of this species of Turkish origin, was isolated and its structure was elucidated as kaempferol-3-O-beta-D-galactoside, a common flavonol derivative by means of spectral techniques.

Topics: Analgesics; Animals; Anti-Inflammatory Agents; Benzoquinones; Biological Assay; Calluna; Carrageenan; Edema; Kaempferols; Male; Mice; Monosaccharides; Pain; Pain Measurement; Plant Components, Aerial; Plant Extracts; Solvents; Toxicity Tests, Acute; Turkey