isoquercitrin and caffeic-acid

Topics: 3,4-Dihydroxyphenylacetic Acid; Benzofurans; Biomass; Caffeic Acids; Chromatography, High Pressure Liquid; Cinnamates; Depsides; Eryngium; Flavonoids; Hydroxybenzoates; Plant Growth Regulators; Plant Shoots; Quercetin; Rosmarinic Acid

This study aimed to clarify the difference between the effective compounds of raw and processed Farfarae flos using a network pharmacology-integrated metabolomics strategy. First, metabolomics data were obtained by ultra high-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF/MS). Then, metabolomics analysis was developed to screen for the influential compounds that were different between raw and processed Farfarae flos. Finally, a network pharmacology approach was applied to verify the activity of the screened compounds. As a result, 4 compounds (chlorogenic acid, caffeic acid, rutin and isoquercitrin) were successfully screened, identified, quantified and verified as the most influential effective compounds. They may synergistically inhibit the p38, JNK and ERK-mediated pathways, which would induce the inhibition of the expression of the IFA virus. The results revealed that the proposed network pharmacology-integrated metabolomics strategy was a powerful tool for discovering the effective compounds that were responsible for the difference between raw and processed Chinese herbs.

Topics: Caffeic Acids; Chlorogenic Acid; Chromatography, High Pressure Liquid; Drug Discovery; Drug Synergism; Drugs, Chinese Herbal; Flowers; Humans; Influenza A virus; Influenza, Human; MAP Kinase Signaling System; Metabolomics; Quercetin; Rutin; Systems Biology; Tandem Mass Spectrometry; Tussilago

Topics: Anthocyanins; Antihypertensive Agents; Antineoplastic Agents, Phytogenic; Antioxidants; Caffeic Acids; Cell Line, Tumor; Centaurea; Chlorogenic Acid; Chromatography, Liquid; Coumaric Acids; Coumarins; Drug Evaluation, Preclinical; Flowers; Hemolysis; Humans; Hydrogen-Ion Concentration; Inhibitory Concentration 50; Lipid Peroxidation; Microbial Sensitivity Tests; Plant Extracts; Quercetin; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Water

To investigate the chemical constituents from Commelina communis, fifteen compounds were separated and purified by silica gel, Sephadex LH-20, and ODS column chromatography, and semi-preparative HPLC. By analyses of NMR and MS data as well as their physical and chemical properties, the structures of these compounds were identified as chrysoeriol-7-O-beta-D-glucoside( 1), methyl gallate(2), p-coumaric acid(3), protocatechuic acid(4), caffeic acid(5), p-hydroxybenzoic acid(6), 2-phenethyl-beta-D-gly-cosidase(7) , rhaponticin(8) , (7S, 8R) -dihydrodehydrodiconiferyl alcohol-9-O-beta-D-glucoside (9), isovitexin (10) , isofurcatain (11), isorhamnetin-3-O-beta-D-glucoside(12) , quercetin-3-O-alpha-L-rhamnoside (13) , isoquercitrin (14) , and 1, 2-dihydro-6, 8-dime-thoxy-7-1-(3, 5-dimethoxy-4-hydroxyphenyl) -N1, N2-bis-[2-( 4-hydroxyphenyl) ethyl] -2, 3-naphthalene dicarboxamide (15). Compounds 2, 5-9, 11, 13 were obtained from the genus Commelina for the first time.

Topics: Caffeic Acids; Chromatography, High Pressure Liquid; Commelina; Glucosides; Hydroxybenzoates; Quercetin

We have studied the perfusion of the jejunum and ileum in an isolated rat intestine model with flavonoids and hydroxycinnamates and the influence of glycosylation on the subsequent metabolism. Flavone and flavonol glucosides and their corresponding aglycones are glucuronidated during transfer across the rat jejunum and ileum and this glucuronidation occurs without the need for gut microflora. Furthermore, this suggests the presence of glycosidases as well as UDP-glucuronyl transferase in the jejunum. In contrast, quercetin-3-glucoside and rutin are mainly absorbed unmetabolised. The results suggest that the more highly reducing phenolics are absorbed predominantly as glucuronides (96.5%+/-4.6) of the amount absorbed, whereas monophenolic hydroxycinnamates and monophenolic B-ring flavonoids are less predisposed to glucuronidation and higher levels of aglycone (88.1%+/-10.1) are detected on absorption through both the jejunum and ileum.

Topics: Animals; Caffeic Acids; Chlorogenic Acid; Coumaric Acids; Flavonoids; Glucuronates; Hesperidin; Ileum; In Vitro Techniques; Intestinal Absorption; Jejunum; Kaempferols; Male; Perfusion; Propionates; Quercetin; Rats; Rats, Sprague-Dawley; Rutin