isopropyl-thiogalactoside and diploptene

isopropyl-thiogalactoside has been researched along with diploptene* in 1 studies

Other Studies

1 other study(ies) available for isopropyl-thiogalactoside and diploptene

Article	Year
Overexpression of squalene-hopene cyclase by the pET vector in Escherichia coli and first identification of tryptophan and aspartic acid residues inside the QW motif as active sites. Bioscience, biotechnology, and biochemistry, 1998, Volume: 62, Issue:2 An overexpression system for squalene-hopene cyclase (SHC) was constructed by using the pET3a vector, which is responsible for high expression with help from the strong T7 promoter when incorporated into E. coli BL21(DE3). Site-directed mutagenesis experiments prove that two amino acid residues of tryptophan and aspartic acid inside the QW-motif 5 resided as active sites. Topics: Amino Acid Sequence; Aspartic Acid; Bacillaceae; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gene Expression Regulation, Bacterial; Genetic Vectors; Intramolecular Transferases; Isopropyl Thiogalactoside; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Plasmids; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Squalene; Transformation, Genetic; Triterpenes; Tryptophan	1998

Article

Year

Overexpression of squalene-hopene cyclase by the pET vector in Escherichia coli and first identification of tryptophan and aspartic acid residues inside the QW motif as active sites.

Bioscience, biotechnology, and biochemistry, 1998, Volume: 62, Issue:2

An overexpression system for squalene-hopene cyclase (SHC) was constructed by using the pET3a vector, which is responsible for high expression with help from the strong T7 promoter when incorporated into E. coli BL21(DE3). Site-directed mutagenesis experiments prove that two amino acid residues of tryptophan and aspartic acid inside the QW-motif 5 resided as active sites.

Topics: Amino Acid Sequence; Aspartic Acid; Bacillaceae; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gene Expression Regulation, Bacterial; Genetic Vectors; Intramolecular Transferases; Isopropyl Thiogalactoside; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Plasmids; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Squalene; Transformation, Genetic; Triterpenes; Tryptophan

1998