isopropyl-thiogalactoside has been researched along with difluprednate* in 1 studies
1 other study(ies) available for isopropyl-thiogalactoside and difluprednate
| Article | Year |
|---|---|
[Preparation and characterization of a recombinant poly-epitopic vaccine EgG1Y162-2 (4) against cystic echinococcosis based on the linker GSGGSG].
To perform prokaryotic expression and preliminary characterization of the recombinant poly-epitope vaccine EgG1Y162-2 (4) against cystic echinococcosis.. The recombinant poly-epitope vaccine EgG1Y162-2 (4) against. The four EgG1Y162-2 proteins coupled by the 3D structure of the recombinant vaccine EgG1Y162-2 (4) presented independent and effective expression and good antigenicity. The highest protein expression was detected in the supernatant following induction of the recombinant plasmid pET30a-EgG1Y162-2 (4) by 0.2 mmol/L IPTG at 37 °C for 4 h, and a pure protein component was seen following elution with 60 mmol/L imidazole. Western blotting analysis of the recombinant multiepitope protein HIS-EgG1Y162-2 (4) showed a band at approximately 39 kDa, and this band was recognized by sera from cystic echinococcosis patients.. A recombinant poly-epitope vaccine EgG1Y162-2 (4) against cystic echinococcosis has been successfully constructed, which provides a preliminary basis for researches on recombinant multi-epitope vaccine against cystic echinococcosis. Topics: Antigens, Helminth; Echinococcosis; Epitopes; Fluprednisolone; Humans; Isopropyl Thiogalactoside; Recombinant Proteins; Vaccines, Synthetic | 2022 |