isochlorogenic-acid and luteolin-7-glucoside

To study the effects of aphid occurring on the quality of Lonicera macranthoides bud.. The fresh and dry bud yields of different aphid damage in the same plant were caculated. Contents of chlorogenic acid caffeic acid, luteolin-7-O-gluco-side, isochlbrogenic acid A, macranthoidin B and dipsacoside B in Lonicera macranthoides bud damaged by aphid were determined by HPLC-ELSD.. Aphid significantly affected the contents of luteolin-7-O-glucoside, macranthoidin B, dipsacoside B, and chlorogenic acid (P < 0. 01 or P <0. 05), but had no obvious effect on the content of caffeic acid and isochlorogenic acid A in the bud of Lonicera macranthoides.. It is supposed to regulate prevention and control of aphids in Lonicera macranthoides GAP base.

Topics: Animals; Aphids; Caffeic Acids; Chlorogenic Acid; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Flavones; Glucosides; Herbivory; Lonicera; Luteolin; Oleanolic Acid; Saponins

To develop a HPLC method quantitative method for simultaneous determination of chlorogenic acid, 1, 5-dicaffeoylquinic acid, isochlorogenic acid A, isochlorogenic acid C, luteolin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside in Chrysanthemum morifolium Ramat.. A Phenomenex Gemini-NX C18 column (4.6 mm x 250 mm, 5 microm) was used with CH3 OH and 0.4% H3PO4 as mobile phases. The flow rate was 1 mL x min(-1), the column temperature was 25 degrees C, and the detection wavelength was set at 350 nm.. The 6 active components were in baseline separation. The linearity of this method was good (r > or = 0.999 7), and the average recoveries were 100.6% - 102.4%, RSD < 3%. Except isochlorogenic acid A, the contents of the determined components in the steam-blanched flower heads were significantly higher than those non blanched. The contents of chlorogenic acid and isochlorogenic acid A in the steam-blanched semiopened flower heads were higher than fully opened ones by 53% and 41%, respectively.. The method is sensitive, accurate, reliable and repeatable, which can be used for quality evaluation of Chrysanthemum.

Topics: Apigenin; Chlorogenic Acid; Chromatography, High Pressure Liquid; Chrysanthemum; Cinnamates; Flowers; Glucosides; Luteolin; Plant Extracts; Quality Control; Reproducibility of Results; Sensitivity and Specificity