irl-1620 and 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene

irl-1620 has been researched along with 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene* in 1 studies

Other Studies

1 other study(ies) available for irl-1620 and 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene

Article	Year
A pivotal role for cADPR-mediated Ca2+ signaling: regulation of endothelin-induced contraction in peritubular smooth muscle cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2002, Volume: 16, Issue:7 cADPR, a potent calcium-mobilizing intracellular messenger synthesized by ADP-ribosyl cyclases regulates openings of ryanodine receptors (RyR). Here we report that in the rat testis, a functional cADPR Ca2+ release system is essential for the contractile response of peritubular smooth muscle cells (PSMC) to endothelin (ET). We previously showed that this potent smooth muscle agonist elicits intracellular Ca2+ release in PSMC and seminiferous tubule contraction via activation of ETA and ETB receptors. ETB-R induces the mobilization of a thapsigargin-sensitive but IP3-independent intracellular Ca2+ pool. Stimulation of permeabilized PSMC with cADPR was found to elicit large Ca2+ releases blocked by either a selective antagonist of cADPR or a RyR blocker, but not by heparin. Western blotting and confocal fluorescence microscopy indicated the specific expression of type 2 RyR in perinuclear localization. ET was found to stimulate the activity of ADP-ribosyl cyclase. Microinjection of the selective cADPR antagonist 8NH2-cADPR completely abolished subsequent stimulation of Ca2+ signaling via ETA and ETB receptors. cADPR therefore appears to have an obligatory role for ETA-R and ETB-R-mediated calcium signaling in PSMC. However, ETB-R seem to be coupled exclusively to cADPR whereas ETA-R activation may be linked to IP3 and cADPR signaling pathways. Topics: Adenosine Diphosphate Ribose; ADP-ribosyl Cyclase; ADP-ribosyl Cyclase 1; Animals; Antigens, CD; Antigens, Differentiation; Blotting, Western; Boron Compounds; Calcium Channels; Calcium Signaling; Cell Membrane; Cells, Cultured; Culture Techniques; Cyclic ADP-Ribose; Endothelin-1; Endothelins; Fluorescent Dyes; Inositol 1,4,5-Trisphosphate Receptors; Male; Membrane Glycoproteins; Muscle Contraction; Muscle, Smooth; NAD+ Nucleosidase; Peptide Fragments; Rats; Receptor, Endothelin B; Receptors, Cytoplasmic and Nuclear; Receptors, Endothelin; Ryanodine; Ryanodine Receptor Calcium Release Channel; Seminiferous Tubules	2002

Article

Year

A pivotal role for cADPR-mediated Ca2+ signaling: regulation of endothelin-induced contraction in peritubular smooth muscle cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2002, Volume: 16, Issue:7

cADPR, a potent calcium-mobilizing intracellular messenger synthesized by ADP-ribosyl cyclases regulates openings of ryanodine receptors (RyR). Here we report that in the rat testis, a functional cADPR Ca2+ release system is essential for the contractile response of peritubular smooth muscle cells (PSMC) to endothelin (ET). We previously showed that this potent smooth muscle agonist elicits intracellular Ca2+ release in PSMC and seminiferous tubule contraction via activation of ETA and ETB receptors. ETB-R induces the mobilization of a thapsigargin-sensitive but IP3-independent intracellular Ca2+ pool. Stimulation of permeabilized PSMC with cADPR was found to elicit large Ca2+ releases blocked by either a selective antagonist of cADPR or a RyR blocker, but not by heparin. Western blotting and confocal fluorescence microscopy indicated the specific expression of type 2 RyR in perinuclear localization. ET was found to stimulate the activity of ADP-ribosyl cyclase. Microinjection of the selective cADPR antagonist 8NH2-cADPR completely abolished subsequent stimulation of Ca2+ signaling via ETA and ETB receptors. cADPR therefore appears to have an obligatory role for ETA-R and ETB-R-mediated calcium signaling in PSMC. However, ETB-R seem to be coupled exclusively to cADPR whereas ETA-R activation may be linked to IP3 and cADPR signaling pathways.

Topics: Adenosine Diphosphate Ribose; ADP-ribosyl Cyclase; ADP-ribosyl Cyclase 1; Animals; Antigens, CD; Antigens, Differentiation; Blotting, Western; Boron Compounds; Calcium Channels; Calcium Signaling; Cell Membrane; Cells, Cultured; Culture Techniques; Cyclic ADP-Ribose; Endothelin-1; Endothelins; Fluorescent Dyes; Inositol 1,4,5-Trisphosphate Receptors; Male; Membrane Glycoproteins; Muscle Contraction; Muscle, Smooth; NAD+ Nucleosidase; Peptide Fragments; Rats; Receptor, Endothelin B; Receptors, Cytoplasmic and Nuclear; Receptors, Endothelin; Ryanodine; Ryanodine Receptor Calcium Release Channel; Seminiferous Tubules

2002