iridoids and vanillin

A sensitive, selective and robust qualitative and quantitative densitometric high-performance thin layer chromatographic method was developed and validated for the determination of iridoid glycoside in the aerial part of Gambhari (Gmelina arborea). Iridoid gycoside 6-O-(2'',3''-dibenzoyl)-alpha-l-rhamnopyranosylcatalpol (IG) was used as a chemical marker for the standardization of G. arborea plant extracts. The separation was performed on aluminum Kieselgel 60F254 TLC plates using chloroform-methanol as mobile phase. The quantitation of IG was carried out using the densitometric reflection/absorption mode at 240 and 430 nm after post-chromatographic derivatization with vanillin-sulphuric acid reagent. A precise and accurate quantification can be performed in the linear working concentration range of 1000-5000 ng/spot with good correlation (r2=0.994). The method was validated for peak purities, precision, robustness, limit of detection (LOD) and quantitation (LOQ), etc., as per ICH guidelines. Specificity of quantitation was confirmed using retention factor (R(f)), UV-vis spectral correlation and ESI-MS spectra of marker compound (IG) in sample track.

Topics: Benzaldehydes; Chloroform; Chromatography, Thin Layer; Evaluation Studies as Topic; Glycosides; Guidelines as Topic; India; Iridoids; Lamiaceae; Methanol; Molecular Structure; Plant Components, Aerial; Plant Extracts; Plants, Medicinal; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Silica Gel; Silicon Dioxide; Spectrometry, Mass, Electrospray Ionization; Sulfuric Acids; Time Factors

A simple and reproducible method for qualitative and quantitative analysis of phenolic compounds in virgin olive oils by solid-phase extraction (SPE), high performance liquid chromatography with diode array detector (HPLC-DAD), and HPLC-mass spectrometry (MS) in tandem mode was developed. The polar fraction was obtained from samples of three different virgin olive oils. Detection and quantification were performed at 280, 240, and 320 nm. For identification purposes, HPLC-MS/MS was equipped with turbo ion spray source in the negative-ion mode. Twenty compounds of twenty-three detected and quantified were characterized. The method showed satisfactory linearity (r > 0.99), good recovery, satisfactory precision, and appropriate limits of detection (LOD) and quantification (LOQ).

Topics: Benzaldehydes; Chromatography, High Pressure Liquid; Flavonoids; Glucosides; Hydroxybenzoates; Iridoid Glucosides; Iridoids; Mass Spectrometry; Olive Oil; Phenols; Plant Oils; Pyrans; Sensitivity and Specificity