iodonaphthylazide has been researched along with 3-(trifluoromethyl)-3-(3-iodophenyl)diazirine* in 1 studies
1 other study(ies) available for iodonaphthylazide and 3-(trifluoromethyl)-3-(3-iodophenyl)diazirine
| Article | Year |
|---|---|
Hydrophobic labelling of membrane-embedded proteins with lipophilic reagents. Incorporation of [125I]INA and [125I]TID into B lymphocyte membrane immunoglobulins.
Hydrophobic labelling is frequently used in the study of membrane-inserted domains of intrinsic proteins. However, the published procedures, fail to incorporate sufficient radioactivity into membrane immunoglobulins of B lymphocytes to permit investigation of their subunit structures and associations with other proteins. In order to increase the specific radioactivity of [125I]iodonaphthylazide ([125I]INA), an improved method for the synthesis of the reagent was developed. In addition, the optimal conditions for labelling B lymphocytes with [125I]INA and the commercially available reagent 3-(trifluoromethyl)-3-(3'-[125I]iodophenyl)diazirine ([125I]TID) were elaborated. Under these optimized conditions, Ig molecules labelled with [125I]INA and [125I]TID were isolated and analysed in detail by SDS-PAGE. The usefulness of the two reagents for the investigation of lipid-embedded domains of membrane proteins is discussed. Topics: Animals; Azides; Azirines; B-Lymphocytes; Iodine Radioisotopes; Membrane Lipids; Membrane Proteins; Mice; Molecular Weight; Receptors, Antigen, B-Cell; Solubility | 1989 |