interleukin-8 and icariin

Topics: Apoptosis; Caspase 3; Caspases, Initiator; Catalase; Cell Proliferation; Cell Survival; Endoplasmic Reticulum Chaperone BiP; Flavonoids; Glutathione Peroxidase; Goblet Cells; Heat-Shock Proteins; Humans; Hydrogen Peroxide; Inflammation; Interleukin-1beta; Interleukin-6; Interleukin-8; L-Lactate Dehydrogenase; Lipopolysaccharides; Malondialdehyde; Mucin-2; Phosphorylation; Reactive Oxygen Species; Superoxide Dismutase-1; Transcription Factor CHOP; Tumor Necrosis Factor-alpha; X-Box Binding Protein 1

To investigate the effects of icariin, a major constituent of flavonoids isolated from the herb Epimedium, on cigarette smoke (CS) induced inflammatory responses in vivo and in vitro.. In vivo, BALB/c mice were exposed to smoke of 15 cigarettes for 1 h/day, 6 days/week for 3 months and dosed with icariin (25, 50 and 100 mg/kg) or dexamethasone (1 mg/kg). In vitro, A549 cells were incubated with icariin (10, 50 and 100 µM) followed by treatments with CSE (2.5%).. We found that icariin significantly protected pulmonary function and attenuated CS-induced inflammatory response by decreasing inflammatory cells and production of TNF-α, IL-8 and MMP-9 in both the serum and BALF of CS-exposed mice and decreasing production of TNF-α and IL-8 in the supernatant of CSE-exposed A549 cells. Icariin also showed properties in inhibiting the phosphorylation of NF-κB p65 protein and blocking the degradation of IΚB-α protein. Further studies revealed that icariin administration markedly restore CS-reduced GR protein and mRNA expression, which might subsequently contribute to the attenuation of CS-induced respiratory inflammatory response.. Together these results suggest that icariin has anti-inflammatory effects in cigarette smoke induced inflammatory models in vivo and in vitro, possibly achieved by suppressing NF-κB activation and modulating GR protein expression.

Topics: Animals; Cell Line, Tumor; Flavonoids; Gene Expression Regulation; Humans; Inflammation; Interleukin-8; Lung; Male; Matrix Metalloproteinase 9; Mice, Inbred BALB C; NF-kappa B; Receptors, Glucocorticoid; Respiratory Function Tests; RNA, Messenger; Smoking; Tumor Necrosis Factor-alpha