interleukin-8 and grepafloxacin

We examined the effect of grepafloxacin (GPFX), a new fluoroquinolone antimicrobial agent, on interleukin-8 (IL-8) expression in tumor necrosis factor-alpha (TNF-alpha)-stimulated human airway epithelial cells (AEC). GPFX inhibited IL-8 protein production as well as mRNA expression in a concentration-dependent manner (2.5 - 25 micro g/ml), but the inhibition of IL-8 expression by corresponding concentrations of GPFX to serum and airway lining fluids was not complete. We discuss the modulatory effect of GPFX on IL-8 production in the context of its efficacy on controlling chronic airway inflammatory diseases.

Topics: Anti-Infective Agents; Cell Survival; Cells, Cultured; Culture Media, Conditioned; Dose-Response Relationship, Drug; Fluoroquinolones; Humans; Interleukin-8; Piperazines; Respiratory Mucosa; RNA, Messenger; Time Factors; Tumor Necrosis Factor-alpha; Up-Regulation