interleukin-8 and fibroblast-growth-factor-(1-10)

To investigate the effects of chitosan on the biological activities of the fibroblasts derived from different tissues.. The biological activities of the fibroblasts derived from different tissues were evaluated with a MTT method for fibroblast proliferation, photic and electronic microscope for morphologic and subcellular structure, 3H-proline uptake method for collagen secretion and ELISA box for the secretion of TGF-beta 1, FGF-AB, and IL-8.. This study showed that the chitosan inhabited the proliferation of the fibroblasts and the secretion of the TGF-beta 1, FGF-AB and collagen of the fibroblasts with a dose-depended manner in the normal skin, hypertrophic scar and keloid groups, but it stimulated the IL-8. However, there were no significant differences among the three groups (P > 0.05).. The chitosan could inhibit the growth, proliferation, biosynthesis and secretion of the fibroblasts, and it may be used to treat different scars.

Topics: Adolescent; Adult; Cell Division; Chitin; Chitosan; Dose-Response Relationship, Drug; Female; Fibroblast Growth Factors; Fibroblasts; Hemostatics; Humans; Interleukin-8; Male; Microscopy, Electron; Peptide Fragments; Transforming Growth Factor beta