interleukin-8 and 6-carboxyfluorescein-diacetate

Pru p 3 is the major peach allergen and the most frequent cause of food allergy in adults in the Mediterranean area. Although its allergenicity is well characterized, its ability to generate a T-cell response is not completely known.. To investigate the influence of Pru p 3 allergen on dendritic cell (DC) maturation and specific T-cell response (T(H)1/T(H)2) in peach allergic patients.. Peach allergic patients (n = 11) and tolerant controls (n = 14) were included in the study. Monocyte-derived DC maturation after incubation with Pru p 3 was evaluated by the increase of maturational markers (CD80, CD86, and CD83) by flow cytometry. Lymphocyte proliferation was evaluated by coculturing monocyte-derived DCs and 5,6-carboxyfluorescein diacetate N-succinimidyl ester-stained lymphocytes with different concentrations of Pru p 3 (25, 10, and 1 μg/mL) by flow cytometry and cytokine production.. Pru p 3 induced a significant increase in the CD80, CD86, and CD83 expression on stimulated DCs from patients compared with controls. The lymphocyte proliferative response after Pru p 3 stimulation was also significantly higher along with an increase in interleukin 8 in patients compared with tolerant controls.. Pru p 3 allergen induces changes in DC maturational status mainly in peach allergic patients. An increase in lymphocyte proliferative response accompanied with a different cytokine pattern was also observed compared with healthy controls.

Topics: Adolescent; Adult; Allergens; Antigens, CD; Antigens, Plant; B7-1 Antigen; B7-2 Antigen; Case-Control Studies; CD83 Antigen; Cell Proliferation; Dendritic Cells; Female; Flow Cytometry; Fluoresceins; Food Hypersensitivity; Humans; Immunoglobulins; Interleukin-8; Lymphocyte Activation; Male; Membrane Glycoproteins; Middle Aged; Monocytes; Plant Proteins; Prunus; Th1 Cells; Th1-Th2 Balance; Th2 Cells