interleukin-8 and 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol

Acute gouty arthritis is an auto-inflammatory disease caused by the deposition of monosodium urate (MSU) crystals in joints or tissues. Excessive neutrophil recruitment into gouty lesions is a general clinical sign and induces a pain phenotype. Attenuation of successive periods of neutrophil infiltration might be a beneficial approach to achieve therapeutic efficacy. In this study, the activity of 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG) in attenuation of excess neutrophil infiltration was assessed in gout-induced lesions of BALB/c mice. Neutrophil infiltration in MSU-induced gouty lesions was analyzed using immunohistochemical staining. ELISA and RT-PCR were used to measure attenuation of expression of the major neutrophil chemoattractant, CXC motif chemokine ligand 8 (CXCL8), in a PLAG-treated animal model and in cells

Topics: Acute Disease; Animals; Arthritis, Gouty; Cell Movement; Diglycerides; Disease Models, Animal; Humans; Inflammation; Interleukin-8; Male; Mice; Mice, Inbred BALB C; Neutrophil Infiltration; Neutrophils; Receptors, Purinergic P2; Signal Transduction; THP-1 Cells; Uric Acid