inosinic-acid has been researched along with 2--deoxyguanosine-5--phosphate* in 2 studies
2 other study(ies) available for inosinic-acid and 2--deoxyguanosine-5--phosphate
| Article | Year |
|---|---|
Analysis of platinum adducts with DNA nucleotides and nucleosides by capillary electrophoresis coupled to ESI-MS: indications of guanosine 5'-monophosphate O6-N7 chelation.
DNA is the ultimate target of platinum-based anticancer therapy. Since the N7 of guanine is known to be the major binding site of cisplatin and its analogues, adduct formation with model nucleotides, especially 2'-deoxyguanosine 5'-monophosphate (dGMP), has been studied in detail. During the last few years a coupled capillary eletrophoresis/electrospray-ionization mass spectrometry (CE/ESI-MS) method has been advantageously used in order to separate and identify platinum adducts with nucleotides in submillimolar concentrations in aqueous solutions. Beside the bisadduct, [Pt(NH(3))(2)(dNMP)(2)](2-) (NMP=2'-deoxynucleoside 5'-monophosphate), and the well-known monochloro and monohydroxo adducts, [Pt(NH(3))(2)Cl(dNMP)](-) and [Pt(NH(3))(2)(dNMP)OH](-), respectively, a third kind of monoadduct species with a composition of [Pt(NH(3))(2)(dNMP)](-) can be separated by CE and detected through the m/z values measured with ESI-MS. Different experimental setups indicate the existence of an O(6)-N7 chelate, whereas the formation of N7-alphaPO(4) macrochelates or dinuclear species is unlikely. Additionally, offline MS experiments with 2'-deoxyguanosine (dG) and stabilization of the controversially discussed O(6)-N7 chelate by oxidation with hydrogen peroxide support the assumption of the existence of O(6)-N7 chelation. Topics: Cisplatin; Deoxyguanine Nucleotides; DNA Adducts; Electrophoresis, Capillary; Guanosine Monophosphate; Inosine Monophosphate; Molecular Structure; Nucleosides; Nucleotides; Organoplatinum Compounds; Spectrometry, Mass, Electrospray Ionization | 2004 |
Enzymatic synthesis of guanine nucleotides labeled with 15N at the 2-amino group of the purine ring.
GMP and dGMP labeled with 15N at the 2-amino group of the purine ring was obtained enzymatically from NH4Cl (> 99 at.% 15N) and from IMP or dIMP, respectively, by several reactions involving IMP-dehydrogenase, GMP-synthetase, adenylate kinase, and creatine kinase. The first three enzymes were obtained by overexpression in Escherichia coli of the corresponding genes. The isotope content of the primary amino group of guanine determined by mass spectrometry after acid hydrolysis of nucleotides was found higher than 98 at.% 15N. The proton NMR spectrum of [15N]GMP in solution in the absence of nitrogen decoupling showed a doublet with a coupling constant of 92 Hz. When nitrogen decoupling was used during the acquisition time, the doublet was replaced by a single peak at 6.47 ppm, indicating that the corresponding proton is bound to 15N. Topics: Adenylate Kinase; Carbon-Nitrogen Ligases; Cloning, Molecular; Creatine Kinase; Deoxyguanine Nucleotides; Escherichia coli; Guanine Nucleotides; Guanosine Monophosphate; IMP Dehydrogenase; Indicators and Reagents; Inosine Monophosphate; Isotope Labeling; Kinetics; Ligases; Mass Spectrometry; Nitrogen Isotopes; Organophosphorus Compounds; Recombinant Proteins | 1995 |