inosine-triphosphate and alpha-beta-methyleneadenosine-5--triphosphate

inosine-triphosphate has been researched along with alpha-beta-methyleneadenosine-5--triphosphate* in 2 studies

Other Studies

2 other study(ies) available for inosine-triphosphate and alpha-beta-methyleneadenosine-5--triphosphate

ArticleYear
Effects of purine and pyrimidine nucleotides on intracellular Ca2+ in human eosinophils: activation of purinergic P2Y receptors.
    The Journal of allergy and clinical immunology, 2001, Volume: 107, Issue:5

    Extracellular adenosine 5'-triphosphate (ATP) increases human eosinophil intracellular Ca(2+) concentration; the mechanism of action is not fully known. ATP, a physiologic regulator, acts through 2 purinergic receptor types: cation channels (P2X) and G protein-coupled receptors (P2Y).. This study is aimed at identifying the functional purinergic receptors in human eosinophils.. The relative potency of ATP, uridine (UTP), cytidine (CTP), and inosine (ITP) 5'-triphosphates (P2Y agonists); 2-methylthio-ATP (P2Y(1) agonist); and 2 P2X agonists, alpha,beta-methylene-ATP and beta,gamma-methylene-ATP on intracellular Ca(2+) concentration was examined in Ca(2+)-sensitive Fura-2-labeled human eosinophils. For comparison, ATP effects were similarly studied in human neutrophils. P2X/P2Y mRNA expression in cells was examined by reverse transcription and PCR.. The nucleotide potency order was UTP > or = ATP > ITP >>> 2-methylthio-ATP > alpha,beta-methylene-ATP = beta,gamma-methylene-ATP = CTP = 0 in eosinophils. Pertussis toxin (500 ng/mL) pretreatment abolished the effect of lower (10(-6) mol/L) but not higher (10(-5) mol/L) concentrations of ATP in eosinophils, whereas it attenuated the effects of 10(-4) mol/L ATP in neutrophils. The phospholipase C inhibitor U73122 (2 micromol/L) partially inhibited the effect of ATP in eosinophils but totally blocked it in neutrophils. Both cells constitutively express mRNA for P2X(1), P2X(4), P2X(5), P2Y(1), and P2Y(2), but not P2X(7), with much weaker expressions of P2X(4) and P2X(5) in neutrophils. Eosinophils cultured with the T(H)1 cytokine, IFN-gamma, expressed mRNA for P2X(7), a receptor linked to apoptosis.. These results suggest that the P2 purinergic receptor signal transduction pathways in eosinophils and neutrophils are different and are mediated by more than 1 subtype of functional P2Y receptors.

    Topics: Adenosine Triphosphate; Asthma; Calcium; Calcium Signaling; Cytidine Triphosphate; Dexamethasone; Eosinophils; Gene Expression Regulation; GTP-Binding Proteins; Humans; Hypersensitivity; Inosine Triphosphate; Interferon-gamma; Ion Transport; Neutrophils; Pertussis Toxin; Receptors, Purinergic P2; Recombinant Proteins; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Thionucleotides; Uridine Triphosphate; Virulence Factors, Bordetella

2001
Extracellular ATP and UTP exert similar effects on rat isolated hepatocytes.
    British journal of pharmacology, 1992, Volume: 105, Issue:2

    1. Extracellular UTP and ATP show obvious similarities in their control of several metabolic functions of rat isolated hepatocytes. 2. They have a similar time-course and concentration-dependency for the activation of glycogen phosphorylase, the generation of inositol trisphosphate (IP3), the inhibition of glycogen synthase and the lowering of adenosine 3':5'-cyclic monophosphate (cyclic AMP) levels. 3. There is a similar synergism of the nucleotides with glucagon in activating phosphorylase. 4. They undergo a similar inhibition by phorbol myristic acid of their glycogenolytic effect. 5. The ATP and UTP effect on IP3 levels are not additive. 6. It is tentatively concluded that UTP and ATP use a common receptor.

    Topics: Adenosine Triphosphate; Animals; Biotransformation; Glycogen; In Vitro Techniques; Inosine Triphosphate; Kinetics; Liver; Male; Phosphorylases; Purinergic Antagonists; Rats; Rats, Inbred Strains; Tetradecanoylphorbol Acetate; Uridine Triphosphate

1992