indigo-carmine and naphthol-yellow

The use of salts as competing agents in staining solutions allows to evaluate to what extent ionic interactions take place between microscopical substrates and cationic or anionic dyes. We have employed this method to study the staining reaction of horse eosinophil leucocyte granules by the anionic dyes nuclear fast red, naphthol yellow S, eosin Y, indigocarmin, acid fuchsin, alizarin red S, orange G, and Evans blue in the presence of NaCl (from 0.015 to 2 mol/l). Different values of "minimal electrolyte concentration" (the least amount of salt which reduces the staining intensity) were found for these dyes. Comparative observations using ammonium sulphate as competing salt showed that it is less effective than NaCl. Staining of eosinophil granules by anionic dyes could be not suppressed at 2 mol/l NaCl, which indicates that in addition to electrostatic forces, other non-ionic interactions are also responsible for some staining reaction of these acidophilic structures.

Topics: Ammonium Sulfate; Animals; Anthraquinones; Azo Compounds; Cytoplasmic Granules; Eosine Yellowish-(YS); Eosinophils; Evans Blue; Horses; Indigo Carmine; Naphthalenesulfonates; Rosaniline Dyes; Sodium Chloride; Staining and Labeling