indigo-carmine has been researched along with indole-2-carboxylic-acid* in 1 studies
1 other study(ies) available for indigo-carmine and indole-2-carboxylic-acid
| Article | Year |
|---|---|
Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids.
The p-cumate-degrading strain Pseudomonas putida F1 and the m- and p-toluate-degrading strain P. putida mt-2 transform indole-2-carboxylate and indole-3-carboxylate to colored products identified here as indigo, indirubin, and isatin. A mechanism by which these products could be formed spontaneously following dioxygenase-catalyzed dihydroxylation of the indolecarboxylates is proposed. Indolecarboxylates were employed as chromogenic substrates for identifying recombinant bacteria carrying genes encoding p-cumate dioxygenase and toluate dioxygenase. Dioxygenase gene-carrying bacteria could be readily distinguished as dark green-blue colonies among other colorless recombinant Escherichia coli colonies on selective agar plates containing either indole-2-carboxylate or indole-3-carboxylate. Topics: Amino Acids; Carboxylic Acids; Chromogenic Compounds; Cloning, Molecular; Coloring Agents; Indigo Carmine; Indoles; Isatin; Oxygenases; Pseudomonas putida | 1995 |