hyperoside and protocatechuic-acid

We have recently demonstrated that the hydroethanolic extracts of Impatiens glandulifera Royle (Balsaminaceae) have antianxiety effect in mice. The present study was aimed to investigate an antidepressant activity of hyperoside (HYP) and protocatechuic acid (PCA), two polyphenols isolated from the aerial parts of this plant, using the forced swimming test (FST) and tail suspension test (TST) in mice. The implication of the monoaminergic system in this effect was assessed and brain-derived neurotrophic factor (BDNF) expression was measured. At doses 1.875, 3.75 and 7.5 mg/kg, HYP and PCA significantly reduced immobility in the FST and TST, without affecting locomotor activity of mice. Pretreatment with p-chlorophenylalanine (PCPA 100 mg/kg, a serotonin synthesis inhibitor) or α-methyl-DL-tyrosine (AMPT 100 mg/kg, a catecholamine synthesis inhibitor) was able to prevent antidepressant-like effect of HYP and PCA (3.75 mg/kg). Sub-effective doses of fluoxetine (5 mg/kg) or reboxetine (2 mg/kg) were capable of potentiating the effect of a sub-effective dose of HYP (0.94 mg/kg) in the FST. Co-administration of sub-effective dose of PCA (0.94 mg/kg) and reboxetine (2 mg/kg) resulted in reducing immobility in the FST. The antidepressant-like effect of HYP and PCA was also prevented by the administration of sulpiride (50 mg/kg), a D2 antagonist. In addition, HYP (3.75 and 7.5 mg/kg) and PCA (7.5 mg/kg) improved the expression of hippocampal BDNF of mice subjected to TST. Altogether, our findings suggest that HYP and PCA exert antidepressant-like effects in mice, which was possibly mediated by monoaminergic system and the upregulation of BDNF level.

Topics: Animals; Antidepressive Agents; Biogenic Amines; Brain-Derived Neurotrophic Factor; Depression; Dose-Response Relationship, Drug; Hydroxybenzoates; Impatiens; Male; Mice; Plant Components, Aerial; Plant Extracts; Quercetin; Swimming

Inula graveolens (L.) Desf. is an annual aromatic herb which has various uses on alternative medicine in many region of the world. In this study, antioxidant activities of ethanol and water extracts of the plant leaves were determined by in vitro DPPH method and phenolic composition of the plant sample was determined by LC-MS/MS analysis. The results showed that chlorogenic acid, quinic acid, hyperoside, protocatechuic acid and quercetin were the major phenolic compounds among the 27 standard compounds. The significant antioxidant capacity of the plant might be related with the high abundance of phenolic compounds.

Topics: Antioxidants; Chlorogenic Acid; Chromatography, High Pressure Liquid; Chromatography, Liquid; Hydroxybenzoates; Inula; Phenols; Plant Extracts; Plant Leaves; Quercetin; Quinic Acid; Tandem Mass Spectrometry

Phenolics, particularly from apples, hold great interest because of their antioxidant properties. In the present study, the total antioxidant capacity of different apple extracts obtained by pressurized hot water extraction (PHWE) was determined by cyclic voltammetry (CV), which was compared with the conventional antioxidant assays. To measure the antioxidant capacity of individual antioxidants present in apple extracts, a novel method was developed based on high-performance liquid chromatography (HPLC) with photodiode array (DAD), electrochemical (ECD), and charged aerosol (CAD) detection. HPLC-DAD-ECD-CAD enabled rapid, qualitative, and quantitative determination of antioxidants in the apple extracts. The main advantage of using CAD was that this detector enabled quantification of a large number of phenolics using only a few standards. The results showed that phenolic acids and flavonols were mainly responsible for the total antioxidant capacity of apple extracts. In addition, protocatechuic acid, chlorogenic acid, hyperoside, an unidentified phenolic acid, and a quercetin derivative presented the highest antioxidant capacities.

Topics: Aerosols; Antioxidants; Chlorogenic Acid; Chromatography, High Pressure Liquid; Electrochemical Techniques; Fruit; Hydroxybenzoates; Malus; Phenols; Plant Extracts; Quercetin; Species Specificity

A simple and accurate liquid chromatographic method was developed for the simultaneous determination of six constituents in the fruit of Acanthopanax sessiliflorus. The conditions of sample extraction were optimised by using orthogonal design. The method provided good accuracy with recoveries in the range of 95.6-101.6% and good precision with RSD values less than 3.0%, which has been successfully applied to the quantitative determination of the six compounds in the fruit of A. sessiliflorus from two maturation periods.

Topics: Chromatography, High Pressure Liquid; Coumarins; Eleutherococcus; Fruit; Furans; Glucosides; Hydroxybenzoates; Lignans; Plant Extracts; Quercetin; Reference Standards