hyperoside and peoniflorin

As one of the new drugs of traditional Chinese medicine, Sanye Tablet is employed as a hypolipidemic in the traditional medicine, but the biopharmaceutical properties of the drug is still unclear.. Through the study of biopharmaceutical properties, the classical biopharmaceutics classification system (BCS) can be used to classify and predict the in vivo absorption properties. On this basis, the biopharmaceutical properties closely related to traditional Chinese medicine preparations are added and a modified BCS model is established to predict and judge the absorption degree of traditional Chinese medicine compound.. Representative components of Sanye Tablet were selected and subjected to different in vitro tests. The experimental results were compared with the results of the BCS to evaluate the accuracy and applicability to Sanye Tablet. We take parameters of dissolution and stability based on product characteristics into account. A "modified-BCS" was developed and the results of the improved method and the classic method were compared. Also the ability of each classification system to predict and determine the extent of absorption of the Chinese herbal compound was investigated based on the absolute bioavailability of representative components.. For classic BCS, the five representative components (except for nuciferine) are all class III, nuciferine is class I/II obtained by Caco-2 cell assay and class III/IV obtained by everted gut sac assay. For modified BCS, paeoniflorin is class III, rutin, hyperoside and salvianolic acid B are class III/IV, and nuciferine is class I/II based on Caco-2 cell assay, class III/IV based on everted gut sac assay. Nuciferine is the best of the five components, with absolute bioavailability reaching 61.91% based on in vivo bioavailability test.. The five representative components (except for nuciferine) are all class III/IV, which correlates well with the absolute bioavailability results and demonstrates that they are poorly absorbed substances. The correlation between the classification results obtained using the "modified-BCS" and absorption in the body is better than the correlation obtained using the classic method, suggesting that the improved BCS is more suitable for the characterization of Sanye Tablet. These results indicate that the oral formulation of Sanye Tablet is a BCS III/IV drug.

Topics: Animals; Aporphines; Biopharmaceutics; Caco-2 Cells; Drugs, Chinese Herbal; Glucosides; Humans; Hypoglycemic Agents; Intestinal Absorption; Male; Medicine, Chinese Traditional; Models, Biological; Monoterpenes; Quercetin; Rats, Sprague-Dawley; Rutin

To study the chemical constituents of Shandanshaoyao Decoction.. The sample was prepared by boiling water, then precipitated by 75% of ethanol. The ethanol soluble fraction was extracted by chloroform, ethyl acetate and n-butanol, respectively. The extracts were isolated using macroreticular resin, silica gel and polyamide column chromatography, and then the chemical constituents were identified by chemical and spectroscopic methods.. Nine compounds were isolated from the n-butanol extract and water soluble fraction, and elucidated as vitexin, quercetin, hyperoside, rutin, paeoniflorin, protocatechaldehyde, (+)-catechin, danshensu and citric acid respectively.. The chemical constituents of traditional Chinese medicinal preparations Shandanshaoyao Decoction were reported for the first time.

Topics: Apigenin; Benzoates; Bridged-Ring Compounds; Crataegus; Drug Combinations; Flavonoids; Glucosides; Monoterpenes; Paeonia; Plants, Medicinal; Quercetin; Salvia miltiorrhiza