hyperoside and kaempferol

We investigated the chemical constituents of the leaves of Psidum littorale, which include 16 flavonoids, including seven flavonols, six flavonoid glycosides and three flavonones. The compounds were isolated by silica gel column chromatography. Their structures were elucidated on the basis of spectral analysis and by comparison with published data. Seven flavonols were kaempferol (1), isorhamnetin (2), myricetin- 3,7,3’-trimethyl ether(3), laricitrin (4), quercetin (5), myricetin (6) and quercein-3,4’-dimethyl ether (7), six flavonoid glycosides were guaijaverin (8), hyperoside (9), 5,4’-dyhydroxy-3,7,5’-methoxyflavone-3’-O-β-D- glucoside (10), laricitrin-3-O-xyloside (11), myricetin-3-O-α-L-rhamnopyranoside (12) and myricetin-3-O-β-D- xyloside (13). Three flavonones were 4’-O-methyldihydroquercetin (14), dihydroapigenin (15) and ampelopsin 4’-O-β-D-glucopyranoside (16). Compound 10 is a new chemical, compounds 2-4, 7, 10-16 were first isolated from this plant. (1)H NMR and (13)C NMR data of compound 11 were not reported in literature.

Topics: Flavonoids; Flavonols; Glycosides; Kaempferols; Plant Leaves; Psidium; Quercetin

To study the chemical consituents of Hypericum ascyron.. The constituents were isolated and purified by chromatography on silica gel; the structure of the compound was determined by MS and NMR spectral analysis.. On the basis of spectroscopic analysis and comparison with the reported data, they were identified as hyperoside( 1),hypercalin B( 2),hypercalin C( 3),1,7-dihydroxyxanthone( 4),2,3-dimethoxyxanthone( 5),1-hydroxy-7-methoxyxanthone( 6),rutin( 7),kaempferol( 8),toxyloxanthone B( 9),quercetin( 10),quercitrin( 11),β-daucosterol( 12) and β-sitosterol( 13).. Compounds 2,3,6 and 9 are obtained from this plant for the first time.

Topics: Acetates; Hypericum; Kaempferols; Quercetin; Rutin; Sitosterols

To establish an UHPLC method for simultaneous determination of six flavonoid components of three closelyrelated plants Agrimonia pilosa,Potentilla chinensis and Potentilla discolor including rutin,hyperoside,cynaroside,quercetin,apigenin and kaempferol. Meanwhile three fresh and dry plants were evaluated to compare the contents of six flavonoid components.. The samples were pretreated with ultrasonic extraction with 70% ethanol for 0. 5 h. The analysis was performed on an Acquity HSS T3( 100 mm × 3. 0 mm,1. 8 μm) column with the mobile phase consisting of acetonitrile and 0. 3% glacial acetic acid aqueous at a flow rate of0. 4 m L / min. The detection wavelength was 360 nm,and the column temperature was 35 ℃.. The contents of rutin and hyperoside were high generally,but the content of kaempferol was extremely low in three closely-related plants. The content of apigenin was0. 028 mg / g in Potentilla chinensis,but not detected in Agrimonia pilosa and Potentilla discolor. The content of cymaroside in Agrimonia pilosa was significantly higher than that in Potentilla chinensis and Potentilla discolor. The fresh plants of Potentilla chinensis and Potentilla discolor contained more flavonoids than oven drying plants.. The similar trend of content change from fresh to dry plant has showed a chemotaxonomic relationship of Potentilla chinensis and Potentilla discolor. The established determination method is simple,rapid and efficient,and is applicable for analysis of the contents of flavonoids in three closely-related plants,which provides the scientific basis for rationalization of using these drugs in clinic.

Topics: Acetonitriles; Agrimonia; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Flavonoids; Glucosides; Kaempferols; Luteolin; Potentilla; Quercetin; Rutin

This study was performed to evaluate the phenolic profile, antioxidant and diuretic effects of black cumin and lady-in-a-mist seeds. In the phenolic profile, differences between the two species are significant. Qualitative and quantitative analyses of the phenolic compounds were performed using a HPLC-UV/MS method. Hyperoside was the only identified flavonoid glycoside (1.08 ± 0.01 μg∙g-1 dw plant material), in the N. damascena extract. Regarding the flavonol profile, kaempferol was identified before the hydrolysis, only in the N. sativa extract (6.06 ± 0.02 μg∙g-1 dw plant material) and quercetin only in N. damascena seeds (14.35 ± 0.02 μg∙g-1 dw plant material). The antioxidant potential of the two species was tested through several electron transfer assays, which indicated, excepting for the FRAP assay, N. damascena as exhibiting a higher free radical scavenging activity. The diuretic activity of the two extracts was tested using a rat-experimental model on acute diuresis. Administration of the ethanolic extract of N. sativa (100 mg∙kg-1) resulted in a significant increase in urine volume, although less than found with the reference drug; in addition N. damascena extract did not present a diuretic effect. In reference to the elimination of Na+, K+ and uric acid, the black cumin extract exhibited a higher natriuretic than kaluretic effect and a similar uricosuric effect with control and N. damascena. For N. damascena, the Na+/K+ ratio was sub unitary, but not due to an increasing of the kaluretic effect, but mostly to a decrease of Na+ excretion.

Topics: Animals; Antioxidants; Biphenyl Compounds; Chromatography, High Pressure Liquid; Diuretics; Flavonoids; Kaempferols; Male; Nigella damascena; Nigella sativa; Picrates; Plant Extracts; Polyphenols; Potassium; Quercetin; Rats; Rats, Wistar; Seeds; Sodium; Species Specificity; Uric Acid

Phenolic compounds in buds and leaves of three varieties of black currant in Finland were identified by HPLC-DAD-ESI-MS/MS. Forty-three phenolic compounds of flavonol glycosides, proanthocyanidins and phenolic acids were found in variety "Mikael" whereas only thirty-five in "Mortti" and "Jaloste n:o 15". Glycosides of quercetin and kaempferol were the major phenolics. Rutin, hyperoside, isoquercitrin, kaempferol-3-O-rutinoise, kaempferol-3-O-glucoside, quercetin-3-O-(6″-malonyl)-glucoside and a kaempferol-malonylhexoside were the most abundant flavonol glycosides. The contents of flavonol glycosides ranged from 1 to 7 mg/g fresh weight in leaves showing typically an increasing trend from July to August, reaching the highest values in early October in "Mikael" and the end of August in "Mortti" and "Jaloste n:o 15". This is the first systematic report of the composition and content of phenolic compounds in buds and leaves of black currant.

Topics: Chromatography, High Pressure Liquid; Finland; Flavonols; Flowers; Glycosides; Kaempferols; Phenols; Plant Leaves; Proanthocyanidins; Quercetin; Ribes; Rutin; Seasons; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry

To study the constituents of ethyl acetate fraction form Hypericum hengshanense.. The constituents were isolated and purified by chromatography on silica gel and their structures were elucidated by MS and NMR spectral analysis.. Ten compounds were isolated and identified as: hyperoside (1), hypericin (2), quercetin (3), quercitrin (4), sesamin (5), betulonic acid (6), rutin (7), kaempferol (8), beta-daucosterol (9), beta-sitosterol (10).. All compounds are obtained from this plant for the first time.

Topics: Acetates; Anthracenes; Hypericum; Kaempferols; Magnetic Resonance Spectroscopy; Perylene; Plant Components, Aerial; Plants, Medicinal; Quercetin; Rutin

The present study was conducted out to determine hyperforin, hypericin, pseudohypericin, chlorogenic acid, rutin, hyperoside, quercitrin, quercetin, kaempferol, apigenin-7-O-glucoside and amentoflavone contents of Hypericum adenotrichum, an endemic plant species to Turkey. The aerial parts representing a total of 30 individuals were collected at full flowering, dried at room temperature and assayed for secondary metabolite concentrations by HPLC. All of the chemicals were detected at various levels except for hyperforin. This is the first report on polar chemistry of this endemic species.

Topics: Anthracenes; Bridged Bicyclo Compounds; Chromatography, High Pressure Liquid; Hypericum; Kaempferols; Perylene; Phloroglucinol; Quercetin; Terpenes; Turkey

A simple and efficient method based on micellar electrokinetic capillary electrophoresis (MECC) with electrochemical detection for the determination of catechin, rutin, hyperin, and kaempferol in Plumula Nelumbini is described. Under optimum conditions, all analytes were well separated within 20 min at a separation voltage of 16 kV in a 60-mmol/L borate running buffer (pH 8.0) containing 20 mmol/L sodium dodecyl sulfate (SDS). The current response was linear over two orders of magnitude with detection limits (S/N = 3) ranging from 6.90 x 10(-8) g/mL to 1.30 x 10(-7) g/mL for all analytes. This work provides a useful method for the analysis of Plumula Nelumbini.

Topics: Buffers; Catechin; Chromatography, Micellar Electrokinetic Capillary; Drugs, Chinese Herbal; Electrodes; Electrophoresis, Capillary; Flavonoids; Hydrogen-Ion Concentration; Kaempferols; Nelumbo; Quercetin; Reproducibility of Results; Rutin; Seeds; Sensitivity and Specificity; Static Electricity

A method of high performance capillary electrophoresis with electrochemical detection (CE-ECD) has been developed for the determination of five bioactive components in Semencustae, namely rutin, hyperoside, kaempferol, p-coumaric acid and quercetin. The effects of several factors such as the acidity and concentration of the running buffer, the separation voltage, the applied potential and the injection time on CE-ECD were investigated. Under the optimized conditions, these five components can be separated in a 50.0 mmol/L borax running buffer (pH 9.0) within 19 minutes. A 300 microm diameter carbon disk electrode was used as the working electrode positioned carefully opposite to the outlet of the capillary in a wall-jet configuration at potential of + 950 mV (vs. saturated calomel electrode as reference electrode, SCE). Good linear relationships were established between the peak current and concentration of analytes over two orders of magnitude. The detection limits (S/N = 3) were 1.93 x 10(-5), 3.55 x 10(-4), 3.65 x 10(-5), 1. 73 x 10(-5) and 1.46 x 10(-4) g/L for rutin, hyperoside, kaempferol, p-coumaric acid and quercetin, respectively. The method has been successfully applied to the determination of these analytes in Semencustae samples after a relatively simple extraction procedure, and the assay results were satisfactory.

Topics: Coumaric Acids; Cuscuta; Electrochemistry; Electrophoresis, Capillary; Kaempferols; Propionates; Quercetin; Reproducibility of Results; Rutin

Six flavonoids were isolated from the seed of Cuscuta australis and four of them were identified as kaempferol, quercetin, astragalin and hyperoside. Hyperoside was obtained from this plant for the first time. In comparison with the flavonoids in C. chinensis, it is found that quercetin and its glycoside are the main flavonoids in C. australis. This result suggests that the flavonoids can be used to distinguish these two medicinal materials.

Topics: Drugs, Chinese Herbal; Flavonoids; Kaempferols; Magnoliopsida; Quercetin; Seeds

Topics: Antitussive Agents; Asthma; Bronchitis; Expectorants; Flavonoids; Galactosides; Humans; Kaempferols; Plant Extracts; Plants, Medicinal; Quercetin; Rutin