hyperoside and astragalin

Topics: Anti-Bacterial Agents; Antioxidants; Bacteria; China; Chlorogenic Acid; Chromatography, High Pressure Liquid; Eucommiaceae; Flavonoids; Fungi; Gutta-Percha; Iridoid Glucosides; Iridoids; Kaempferols; Lignans; Metabolome; Phenols; Plant Bark; Plant Extracts; Plant Leaves; Plants, Medicinal; Quercetin; Rutin

A method based on ultra performance liquid chromatography with a diode array detector (UPLC-DAD) was developed for quantitative analysis of five active compounds and chemical fingerprint analysis of

Topics: China; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Ellagic Acid; Gallic Acid; Humans; Kaempferols; Quality Control; Quercetin; Reproducibility of Results; Rosa; Sensitivity and Specificity

A simple, inexpensive and efficient method based on the mixed cloud point extraction (MCPE) combined with high performance liquid chromatography was developed for the simultaneous separation and determination of six flavonoids (rutin, hyperoside, quercetin-3-O-sophoroside, isoquercitrin, astragalin and quercetin) in Apocynum venetum leaf samples. The non-ionic surfactant Genapol X-080 and cetyl-trimethyl ammonium bromide (CTAB) was chosen as the mixed extracting solvent. Parameters that affect the MCPE processes, such as the content of Genapol X-080 and CTAB, pH, salt content, extraction temperature and time were investigated and optimized. Under the optimized conditions, the calibration curve for six flavonoids were all linear with the correlation coefficients greater than 0.9994. The intra-day and inter-day precision (RSD) were below 8.1% and the limits of detection (LOD) for the six flavonoids were 1.2-5.0 ng mL(-1) (S/N=3). The proposed method was successfully used to separate and determine the six flavonoids in A. venetum leaf samples.

Topics: Apocynum; Cetrimonium; Cetrimonium Compounds; Chromatography, High Pressure Liquid; Flavonoids; Hydrogen-Ion Concentration; Kaempferols; Plant Extracts; Plant Leaves; Polyethylene Glycols; Quercetin; Rutin; Surface-Active Agents

To establish HPLC fingerprint of Bidens biternata from different habitats and determine the contents of hyperoside, isoquercetin, astragalin and bipinnatapolyacetylpside.. Analysis was carried on Hypersil ODS C18 column (4.6 mm x 250 mm, 5.0 microm) with acetonitrile and 3% acetic acid as the mobile phase in a gradient elution. The contents of 4 components were determined simultaneously.. The fingerprint of 10 populations were established and the data were analyzed by the similarity evaluation software. There were almost no differences between the similarities of 10 population, but the contents of 4 main compoerls were different among them.. This method is stable and reliable which could be applied in quality assessment.

Topics: Bidens; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Ecosystem; Flavones; Kaempferols; Plant Components, Aerial; Plants, Medicinal; Quality Control; Quercetin; Reproducibility of Results; Solvents

Six phenolics were obtained from the leaves of Prunus padus by activity-guided isolation: isorhamnetin 3-O-β-xylopyranosyl-(1 → 2)-β-galactopyranoside (1), astragalin (2), hyperoside (3), quercetin 3-O-β-xylopyranosyl-(1 → 2)-β-galactopyranoside (4), quercetin 3-O-β-xylopyranosyl-(1 → 2)-β-glucopyranoside (5) and chlorogenic acid (6). The antioxidant potential of 70% methanolic extracts from the flowers and leaves collected over the growing season was evaluated using the 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging and 2,2′-azobis-(2-amidinopropane) dihydrochloride (AAPH)-induced linoleic acid (LA) peroxidation tests in relation to the contents of the isolates 1-6, total phenolics, total proanthocyanidins and total quercetin. The IC₅₀ values were expressed in gram dry weight per gram of DPPH or LA, respectively, and were in the range of 1.42-2.42 for the DPPH test and 1.78-4.92 for the LA peroxidation, with superior activity found for the flowers and the autumn leaves. Significant linear correlation of these values to the sum of proanthocyanidins and compounds 1-6 (R² > 0.87) showed that the listed phenolics are synergists of the tested activity.

Topics: Antioxidants; Biphenyl Compounds; Chlorogenic Acid; Chromatography, High Pressure Liquid; Flowers; Free Radical Scavengers; Glycosides; Inhibitory Concentration 50; Kaempferols; Magnetic Resonance Spectroscopy; Mass Spectrometry; Phenols; Picrates; Plant Extracts; Plant Leaves; Prunus; Quercetin; Ultraviolet Rays

To study the chemical constituents from flowers of Koelreuteria paniculata.. Column chromatography and spectral analysis were used to isolate and identify the constituents.. The EtOAc fraction from flowers of Koelreuteria paniculata was separated and purified. Nine compounds were obtained and identified as:sitosterol glucoside (I), gallic acid (II), kaempferol (III), luteolin (IV), kaempferol-3-O-(6"-acetyl)-beta-D-glucopyranoside (V), hyperoside-2"-O-acetyl (VI), hyperoside-2"-O-galloyl (VII), hyperoside (VIII), kaempferol-3-O-D-glucopyranoside (IX).. Nine compounds are isolated for the first time from flowers of Koelreuteria paniculata. Compounds IV, V, VI and IX are isolated from this genus for the first time.

Topics: Flavonoids; Flowers; Gallic Acid; Kaempferols; Luteolin; Magnetic Resonance Spectroscopy; Molecular Structure; Plants, Medicinal; Quercetin; Sapindaceae; Sitosterols

A new flavonol glycoside 7-O-methyl-kaempferol 4'-beta-D-galactopyranoside (rhamnocitrin 4'-beta-D-galactopyranoside) (1) was isolated from the aerial parts of Astragalus hamosus. The known flavonols hyperoside (2), isoquercitrin (3) and astragalin (4) were also identified. Structures of the compounds were elucidated by chemical and spectral methods.

Topics: Astragalus Plant; Chromatography, High Pressure Liquid; Flavonoids; Glucosides; Kaempferols; Magnetic Resonance Spectroscopy; Molecular Structure; Quercetin

To study the chemical constituents from leaves of Sapium sebiferum.. The compounds were isolated and purified by silic gel column chromatography and preparative HPLC. The structures were identified by various spectral evidence.. Nine compounds were obtained and they were shikimic acid (1), kaempferol (2), quercetin (3), isoquercein (4), hyperin (5), kaempferol-3-O-beta-D-glucopyranoside (6), kaempferol-3-O-beta-D-glueopyranoside (7), gallic acid (8), rutin (9).. Compounds 1, 5, 6 and 7 are isolated from this genus for the first time and compound 9 is isolated from this plant for the first time.

Topics: Chromatography, Gel; Chromatography, High Pressure Liquid; Kaempferols; Plant Leaves; Plants, Medicinal; Quercetin; Rutin; Sapium; Shikimic Acid