hydroxysafflor-yellow-a and puerarin

A simple, precise and reliable LC-MS/MS method was developed and validated for simultaneous quantification of vitexin, notoginsenoside R1, hydroxysafflor yellow A, ginsenoside Rd, puerarin, daidzein and senkyunolide I as components of Naodesheng (NDS) in rat serum. The Linearity ranges in rat serum were 0.045-4.5 μg/mL for vitexin, 0.0476-4.76 μg/mL for notoginsenoside R1, 0.0422-4.22 μg/mL for hydroxysafflor yellow A, 0.0426-4.26 μg/mL for ginsenoside Rd, 0.0436-4.36 μg/mL for puerarin, 0.026-2.6 μg/mL for daidzein, and 0.05-5 μg/mL for senkyunolide I, with the correlation coefficients greater than 0.99. The established method was validated in terms of intra- and inter-day precision and accuracy, recovery, matrix effect and stability. Furthermore, the method was successfully applied for pharmacokinetic study of these seven components in rat serum after oral administration of NDS.

Topics: Administration, Oral; Animals; Benzofurans; Calibration; Chalcone; Chromatography, Liquid; Drugs, Chinese Herbal; Ginsenosides; Isoflavones; Limit of Detection; Linear Models; Quinones; Rats; Rats, Wistar; Reproducibility of Results; Tandem Mass Spectrometry; Time Factors

A high-performance liquid chromatograph with mass spectrum detection (HPLC-MS/APCI) method has been established for simultaneous determination of ten major bioactive components of Naodesheng injection including safflor yellow A, puerarin, daidzein, ginsenosides (Rg1, Rg2, Rb1, Rd, Re, Rh1), and notoginsenoside R1. The separations were carried out with a Luna C18 column (5 microm, 150x4.6 mm, Phenomenex, U.S.A.) with a stepwise gradient elution of the mobile phase consisting of water (0.1% of formic acid, v/v)-methanol (0 min, 70:30; 8 min, 30:70; 20 min, 10:90) at a flow-rate of 0.8 ml/min. The proposed method was applied to analyze five various Naodesheng injections and produced data with acceptable linearity, repeatability, precision and accuracy having lower limits of quantitation (LLOQs) of 0.02-0.2 microg. The calibration curves were linear in respective range for all compounds, all of them with coefficients of determination above 0.9900. The intraday precessions were less than 5.0%. The proposed method is accurate, sensitive and simple, a useful alternative for routine analysis in the quality control of Traditional Chinese Medicine.

Topics: Calibration; Chalcone; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Ginsenosides; Isoflavones; Mass Spectrometry; Quinones; Reproducibility of Results; Sensitivity and Specificity