hydroxymuconic-semialdehyde and aniline

hydroxymuconic-semialdehyde has been researched along with aniline* in 1 studies

Other Studies

1 other study(ies) available for hydroxymuconic-semialdehyde and aniline

Article	Year
A high level of accumulation of 2-hydroxymuconic 6-semialdehyde from aniline by the transpositional mutant Y-2 of Pseudomonas species AW-2. Microbiological research, 1997, Volume: 152, Issue:2 Four transpositional mutants of aniline-assimilating Pseudomonas sp. AW-2 produced 2-hydroxymuconic 6-semialdehyde (HMS) from aniline and accumulated it in a cultural medium. Among the four mutants, strain Y-2 produced the greatest amount of HMS (0.77 mg/ml) from 1 mg/ml of aniline hydrochloride in a 15-h growing culture. The conversion rate of aniline to HMS was 70% on a molar basis. Resting cells of strain Y-2 produced 0.65 mg/ml of HMS during 4h of incubation in a reaction mixture containing 1 mg/ml of aniline hydrochloride (conversion rate, 60%). Transposon Tn5-Mob was found to be inserted into the gene of HMS dehydrogenase in strain Y-2 by sequence analysis. Topics: Alcohol Oxidoreductases; Aldehyde Oxidoreductases; Amino Acid Sequence; Aniline Compounds; Base Sequence; Cloning, Molecular; Fatty Acids, Unsaturated; Molecular Sequence Data; Mutagenesis, Insertional; Mutation; Pseudomonas; Sequence Analysis, DNA	1997

Article

Year

A high level of accumulation of 2-hydroxymuconic 6-semialdehyde from aniline by the transpositional mutant Y-2 of Pseudomonas species AW-2.

Microbiological research, 1997, Volume: 152, Issue:2

Four transpositional mutants of aniline-assimilating Pseudomonas sp. AW-2 produced 2-hydroxymuconic 6-semialdehyde (HMS) from aniline and accumulated it in a cultural medium. Among the four mutants, strain Y-2 produced the greatest amount of HMS (0.77 mg/ml) from 1 mg/ml of aniline hydrochloride in a 15-h growing culture. The conversion rate of aniline to HMS was 70% on a molar basis. Resting cells of strain Y-2 produced 0.65 mg/ml of HMS during 4h of incubation in a reaction mixture containing 1 mg/ml of aniline hydrochloride (conversion rate, 60%). Transposon Tn5-Mob was found to be inserted into the gene of HMS dehydrogenase in strain Y-2 by sequence analysis.

Topics: Alcohol Oxidoreductases; Aldehyde Oxidoreductases; Amino Acid Sequence; Aniline Compounds; Base Sequence; Cloning, Molecular; Fatty Acids, Unsaturated; Molecular Sequence Data; Mutagenesis, Insertional; Mutation; Pseudomonas; Sequence Analysis, DNA

1997