hydroxylamine has been researched along with diethyl pyrocarbonate in 94 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 35 (37.23) | 18.7374 |
| 1990's | 51 (54.26) | 18.2507 |
| 2000's | 8 (8.51) | 29.6817 |
| 2010's | 0 (0.00) | 24.3611 |
| 2020's | 0 (0.00) | 2.80 |
| Authors | Studies |
|---|---|
| Deyashiki, Y; Hara, A; Nakayama, T; Sawada, H; Shinoda, M | 1 |
| Johnston, BH | 1 |
| Cheon, HG; Hanna, PE | 1 |
| Ferl, RJ; Paul, AL | 1 |
| Darnay, BG; Rodwell, VW; Wang, Y | 1 |
| Jacobson, KA; Ji, XD; Stiles, GL | 1 |
| Nimmo, HG; Robertson, AG; Rua, J | 1 |
| Cescatti, L; Menestrina, G; Pederzolli, C | 1 |
| Bang, SK; Kim, YI; Kim, YS | 1 |
| Billi de Catabbi, S; Ríos de Molina, MC; San Martín de Viale, LC | 1 |
| Stoeva, S | 1 |
| Beaumier, B; Béliveau, R | 1 |
| Belguise-Valladier, P; Fuchs, RP | 1 |
| Ellison, MJ; Johnston, BH; Quigley, GJ; Rich, A | 1 |
| Bauer, PI; Buki, KG; Kun, E | 1 |
| Lauf, PK | 1 |
| Blanke, SR; Hager, LP | 1 |
| Hamasaki, N; Izuhara, K; Kanazawa, Y; Kleps, RA; Okubo, K; Omachi, A | 1 |
| Morjana, NA; Scarborough, GA | 1 |
| Cheng, KC; Nowak, T | 1 |
| Bhattacharyya, A; Lilley, DM | 1 |
| Hori, R; Inui, K; Kato, M; Katsura, T; Maegawa, H | 1 |
| Hori, R; Inui, K; Kato, M; Maegawa, H; Okano, T | 1 |
| Rao, NA; Vijayalakshmi, D | 1 |
| Balasubramanian, AS; Boopathy, R | 1 |
| Cook, PF; Harris, BG; Hofer, HW; Rao, GS; Wariso, BA | 1 |
| Aronson, PS; Grillo, FG | 1 |
| Hellenga, K; Mehta, J; Spilberg, I | 1 |
| Berrocal, F; Carreras, J | 1 |
| Borgna, JL; Scali, J | 1 |
| Nattie, EE | 2 |
| Branks, MJ; Masouredis, SP; Victoria, EJ | 1 |
| DeMichele, A; Kinnunen, PM; Lange, LG | 1 |
| Leng, M; Malfoy, B; Rousseau, N; Vogt, N | 1 |
| Huynh, QK | 1 |
| Holohan, PD; Ross, CR; Sokol, PP | 1 |
| Girbig, F; Kramer, W; Leipe, I; Petzoldt, E | 1 |
| Abdulwajid, AW; Wu, FY | 1 |
| Awasthi, YC; Bhatnagar, A; Singh, SV | 1 |
| Poirée, JC; Starita-Géribaldi, M; Sudaka, P | 1 |
| Hatefi, Y; Hederstedt, L | 1 |
| Bui, QT; Garrard, LJ; Nygaard, R; Raushel, FM | 1 |
| Cook, PF; Harris, BG; Rao, JG | 1 |
| Johnston, BH; Rich, A | 1 |
| Hatefi, Y; Vik, SB; Yagi, T | 1 |
| Hatefi, Y; Vik, SB | 1 |
| Zanetti, G | 1 |
| Broquet, P; Louisot, P; Serres-Guillaumond, M | 1 |
| Bindslev, N; Wright, EM | 1 |
| Baker, ME; Terry, LS | 1 |
| Diener, TO; McKinley, MP; Prusiner, SB | 1 |
| Dickerman, HW; Gross, SC; Kumar, SA | 1 |
| de Windt, FE; van der Drift, C; Xu, Z | 1 |
| Donnelly, JL; Pallotta, BS | 1 |
| Balkovetz, DF; Wang, D; Warnock, DG | 1 |
| Bernardi, P; Nicolli, A; Petronilli, V | 1 |
| Baldwin, JE; John, P; Schofield, CJ; Thomas, P; Zhang, Z | 1 |
| Lei, Y; Liu, HW; Ploux, O | 1 |
| Davis-Bruno, K; Halushka, PV; Schrör, K | 1 |
| Barrett, J; Crowley, P; Walker, J | 1 |
| Hughes, MA; Keresztessy, Z; Kiss, L | 1 |
| Albert, AC; Bouche, G; Leng, M; Rahmouni, AR; Roman, AM | 1 |
| Ali, R; Shah, MA | 1 |
| Aguilar, J; Cabiscol, E; Ros, J | 1 |
| Battaglia, E; Fournel-Gigleux, S; Magdalou, J; Ouzzine, M; Pritchard, M; Radominska, A; Siest, G | 1 |
| Bush, DR | 1 |
| Chang, GG; Chiou, SH; Lee, HJ | 1 |
| Jakobs, KH; Kaldenberg-Stasch, S; Nürnberg, B; Schultz, G; Ulibarri, I; Wieland, T | 1 |
| Chang, LH; Tam, MF | 1 |
| Mohammadkhani, R; Said, HM | 1 |
| Antón, LC; Barrio, E; Gavilanes, F; Marqués, G; Ruiz, S; Sánchez, A; Vivanco, F | 1 |
| Lim, J; Turner, AJ | 1 |
| Borders, CL; Chen, LH; Kenyon, GL; Vásquez, JR | 1 |
| Fothergill-Gilmore, LA; Nairn, J; Price, NC; Price, RM | 1 |
| Katiyar, SS; Mukherjee, S | 1 |
| Bhattacharyya, DK; Kwon, O; Meganathan, R | 1 |
| Kim, H; Yang, CH; Yoon, BY | 1 |
| Erlichman, JS; Leiter, JC; Lu, DC | 1 |
| Makarewicz, W; Newby, AC; Skladanowski, AC | 1 |
| Burton, MD; Kazemi, H; Krause, WL | 1 |
| Rai, SS; Wolff, J | 1 |
| Pathre, UV; Sane, PV; Sinha, AK | 1 |
| Howe, DL; Salleh, HM; Sheflyan, GY; Son, JK; Tayeh, MA; Woodard, RW | 1 |
| Busto, F; De Arriaga, D; Olano, J; Soler, J | 1 |
| Castilho, RF; Kowaltowski, AJ; Teixeira, BM; Vercesi, AE | 1 |
| Hsiao, YY; Hung, HH; Pan, RL; Van, RC | 1 |
| Burgner, JW; Gwynn, MN; Hedl, M; Lane, P; Lehnbeuter, KR; Mazzulla, M; McDevitt, D; Rodwell, VW; Stauffacher, CV; Sutherlin, A; Wilding, EI | 1 |
| Carvajal, N; Fuentes, M; López, V; Orellana, MS; Salas, M; Uribe, E | 1 |
| Bandyopadhyay, D; Bandyopadhyay, U; Banerjee, RK; Mazumdar, A | 1 |
| Asai, H; Fang, J; Zhang, B | 1 |
| Chang, CH; Chen, YJ; Huang, CY; Ko, CY; Liao, HK; Wu, TK | 1 |
| Chen, GJ; Goodall, JJ; Page, MG | 1 |
| Ghosh, S; Ray, M; Ray, S | 1 |
94 other study(ies) available for hydroxylamine and diethyl pyrocarbonate
| Article | Year |
|---|---|
Modification of pig liver dimeric dihydrodiol dehydrogenase with diethylpyrocarbonate and by rose bengal-sensitized photooxidation: evidence for an active-site histidine residue.
Topics: Alcohol Oxidoreductases; Animals; Binding Sites; Diethyl Pyrocarbonate; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Liver; Macromolecular Substances; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Photochemistry; Rose Bengal; Spectrometry, Fluorescence; Swine; Tyrosine | 1992 |
Hydroxylamine and methoxylamine as probes of DNA structure.
Topics: Animals; Autoradiography; Base Sequence; Cytosine; Diethyl Pyrocarbonate; DNA; Electrophoresis; Humans; Hydroxylamine; Hydroxylamines; Indicators and Reagents; Molecular Sequence Data; Nucleic Acid Conformation; Plasmids | 1992 |
Effect of group-selective modification reagents on arylamine N-acetyltransferase activities.
Topics: Animals; Arginine; Arylamine N-Acetyltransferase; Binding Sites; Cricetinae; Cysteine; Diethyl Pyrocarbonate; Enzyme Activation; Ethylmaleimide; Histidine; Hydroxylamine; Hydroxylamines; Liver; Male; Mesocricetus; Phenylglyoxal | 1992 |
Chemical detection of Z-DNA within the maize Adh1 promoter.
Topics: Alcohol Dehydrogenase; Base Sequence; Diethyl Pyrocarbonate; DNA, Superhelical; Hydroxylamine; Hydroxylamines; Molecular Sequence Data; Nucleic Acid Conformation; Osmium Tetroxide; Promoter Regions, Genetic; Zea mays | 1992 |
Identification of the catalytically important histidine of 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
Topics: Amino Acid Sequence; Base Sequence; Catalysis; Chromatography, DEAE-Cellulose; Diethyl Pyrocarbonate; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Hydroxymethylglutaryl CoA Reductases; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligonucleotides; Pseudomonas; Substrate Specificity | 1992 |
Chemical modification and irreversible inhibition of striatal A2a adenosine receptors.
Topics: Adenosine; Adenosine-5'-(N-ethylcarboxamide); Affinity Labels; Amino Acid Sequence; Animals; Corpus Striatum; Diethyl Pyrocarbonate; Drug Antagonism; Hydroxylamine; Hydroxylamines; Molecular Sequence Data; Phenethylamines; Purinergic Antagonists; Rabbits; Radioligand Assay; Receptors, Purinergic; Theophylline; Xanthines | 1992 |
Identification of the histidine residue in Escherichia coli isocitrate lyase that reacts with diethylpyrocarbonate.
Topics: Amino Acid Sequence; Binding Sites; Diethyl Pyrocarbonate; Enzyme Reactivators; Escherichia coli; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Isocitrate Lyase; Isocitrates; Kinetics; Molecular Sequence Data; Peptide Fragments; Sulfhydryl Compounds; Trypsin | 1992 |
Modification of lysine residues of Staphylococcus aureus alpha-toxin: effects on its channel-forming properties.
Topics: Bacterial Toxins; Diethyl Pyrocarbonate; Electrochemistry; Electrophoresis, Polyacrylamide Gel; Hemolysin Proteins; Histidine; Hydroxylamine; Hydroxylamines; Ion Channels; Isoelectric Focusing; Lipid Bilayers; Lysine; Staphylococcus aureus; Trinitrobenzenesulfonic Acid | 1991 |
Chemical modification of Pseudomonas fluorescens malonyl-CoA synthetase by diethylpyrocarbonate: kinetic evidence for an essential histidyl residue on alpha subunit.
Topics: Adenosine Triphosphate; Bacterial Proteins; Binding Sites; Coenzyme A; Coenzyme A Ligases; Diethyl Pyrocarbonate; Enzyme Reactivators; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Macromolecular Substances; Malonates; Pseudomonas fluorescens | 1991 |
Studies on the active centre of rat liver porphyrinogen carboxylase in vivo effect of hexachlorobenzene.
Topics: Animals; Arginine; Binding Sites; Carboxy-Lyases; Diethyl Pyrocarbonate; Epoxy Compounds; Female; Hexachlorobenzene; Histidine; Hydroxylamine; Hydroxylamines; Liver; Methylene Blue; Photochemistry; Porphyrias; Rats; Rats, Inbred Strains; Spectrophotometry; Uroporphyrinogens | 1991 |
Modification of a zinc proteinase from Bacillus mesentericus strain 76 by diethylpyrocarbonate.
Topics: Amino Acid Sequence; Bacillus; Binding, Competitive; Chymotrypsin; Diethyl Pyrocarbonate; Histidine; Hydroxylamine; Hydroxylamines; Kinetics; Metalloendopeptidases; Molecular Sequence Data; Phenylalanine | 1991 |
Kidney brush-border membrane transporters: differential sensitivity to diethyl pyrocarbonate.
Topics: Amino Acids; Animals; Biological Transport; Carrier Proteins; Diethyl Pyrocarbonate; Glucose; Hydroxylamine; Hydroxylamines; In Vitro Techniques; Kidney; Microvilli; Phosphates; Rats; Sodium | 1991 |
Strong sequence-dependent polymorphism in adduct-induced DNA structure: analysis of single N-2-acetylaminofluorene residues bound within the NarI mutation hot spot.
Topics: 2-Acetylaminofluorene; Acetaldehyde; Autoradiography; Bacterial Proteins; Base Sequence; Cross-Linking Reagents; Diethyl Pyrocarbonate; DNA, Bacterial; Hydroxylamine; Hydroxylamines; Molecular Probes; Molecular Sequence Data; Mutagenesis; Nucleic Acid Conformation; Nucleosides; Osmium Tetroxide; Phenanthrolines; Polymorphism, Genetic | 1991 |
The Z-Z junction: the boundary between two out-of-phase Z-DNA regions.
Topics: Base Sequence; Computer Graphics; Diethyl Pyrocarbonate; DNA; DNA, Recombinant; Electrophoresis, Gel, Two-Dimensional; Hydroxylamine; Hydroxylamines; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Thermodynamics | 1991 |
Evidence for the participation of histidine residues located in the 56 kDa C-terminal polypeptide domain of ADP-ribosyl transferase in its catalytic activity.
Topics: Binding Sites; Cellulose; Diethyl Pyrocarbonate; DNA; Histidine; Hydroxylamine; Hydroxylamines; Kinetics; Light; Poly(ADP-ribose) Polymerase Inhibitors; Poly(ADP-ribose) Polymerases; Protein Binding; Rose Bengal | 1990 |
Thiol-dependent passive K: Cl transport in sheep red blood cells: X. A hydroxylamine-oxidation induced K: Cl flux blocked by diethylpyrocarbonate.
Topics: Animals; Biological Transport; Carrier Proteins; Chlorides; Diethyl Pyrocarbonate; Erythrocytes; Hydroxylamine; Hydroxylamines; K Cl- Cotransporters; Kinetics; Oxidation-Reduction; Potassium; Sheep; Sulfhydryl Compounds; Symporters | 1990 |
Chemical modification of chloroperoxidase with diethylpyrocarbonate. Evidence for the presence of an essential histidine residue.
Topics: Amino Acid Sequence; Binding Sites; Chemical Phenomena; Chemistry; Chloride Peroxidase; Diethyl Pyrocarbonate; Formates; Fungi; Histidine; Hydroxylamine; Hydroxylamines; Kinetics; Models, Molecular; Molecular Sequence Data; Peroxidases; Protein Conformation; Spectrophotometry, Ultraviolet; Structure-Activity Relationship | 1990 |
Inhibition of chloride binding to the anion transport site by diethylpyrocarbonate modification of Band 3.
Topics: Anion Exchange Protein 1, Erythrocyte; Binding Sites; Biological Transport; Chlorides; Diethyl Pyrocarbonate; Erythrocyte Membrane; Formates; Humans; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Magnetic Resonance Spectroscopy | 1990 |
Evidence for an essential histidine residue in the Neurospora crassa plasma membrane H+-ATPase.
Topics: Binding Sites; Catalysis; Cell Membrane; Diethyl Pyrocarbonate; Formates; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Neurospora; Neurospora crassa; Proton-Translocating ATPases; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds | 1989 |
A histidine residue at the active site of avian liver phosphoenolpyruvate carboxykinase.
Topics: Animals; Chickens; Circular Dichroism; Diethyl Pyrocarbonate; Formates; Histidine; Hydroxylamine; Hydroxylamines; Kinetics; Manganese; Mitochondria, Liver; Phosphoenolpyruvate Carboxykinase (GTP); Protein Binding; Protein Conformation | 1989 |
Single base mismatches in DNA. Long- and short-range structure probed by analysis of axis trajectory and local chemical reactivity.
Topics: Base Composition; Coliphages; Cytosine; Diethyl Pyrocarbonate; DNA; Electrophoresis, Polyacrylamide Gel; Guanine; Hydroxylamine; Hydroxylamines; Nucleic Acid Hybridization; Osmium Tetroxide; Plasmids; Thymine | 1989 |
Inhibitory effect of diethyl pyrocarbonate on the H+/organic cation antiport system in rat renal brush-border membranes.
Topics: Animals; Biological Transport; Cations; Diethyl Pyrocarbonate; Dithiothreitol; Formates; Hydrogen; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Imidazoles; Kidney Cortex; Kinetics; Male; Microvilli; p-Aminohippuric Acid; Rats; Rats, Inbred Strains; Sulfhydryl Reagents; Tetraethylammonium; Tetraethylammonium Compounds | 1989 |
Effect of various chemical modifiers on H+ coupled transport of cephradine via dipeptide carriers in rabbit intestinal brush-border membranes: role of histidine residues.
Topics: Animals; Biological Transport; Cephalosporins; Cephradine; Diethyl Pyrocarbonate; Dipeptides; Dithiothreitol; Dose-Response Relationship, Drug; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Intestinal Mucosa; Male; Microvilli; Rabbits | 1989 |
Identification of amino acid residues at the active site of human liver serine hydroxymethyltransferase.
Topics: Amino Acids; Arginine; Binding Sites; Bromosuccinimide; Diethyl Pyrocarbonate; Glycine Hydroxymethyltransferase; Histidine; Humans; Hydroxylamine; Hydroxylamines; Liver; Phenylglyoxal; Transferases; Tryptophan | 1989 |
Chemical modification of the bifunctional human serum pseudocholinesterase. Effect on the pseudocholinesterase and aryl acylamidase activities.
Topics: 2-Hydroxy-5-nitrobenzyl Bromide; Acetic Anhydrides; Amidohydrolases; Bromosuccinimide; Butyrylcholinesterase; Choline; Cholinesterase Inhibitors; Cholinesterases; Diethyl Pyrocarbonate; Electrophoresis, Polyacrylamide Gel; Humans; Hydroxylamine; Hydroxylamines; Imidazoles; Imipramine; Pentanones; Phenothiazines; Phenylglyoxal; Substrate Specificity; Tetranitromethane; Trinitrobenzenesulfonic Acid | 1985 |
Reaction of Ascaris suum phosphofructokinase with diethylpyrocarbonate. Inactivation and desensitization to allosteric modulation.
Topics: Allosteric Regulation; Allosteric Site; Animals; Ascaris; Diethyl Pyrocarbonate; Formates; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Phosphofructokinase-1 | 1987 |
Inactivation of the renal microvillus membrane Na+-H+ exchanger by histidine-specific reagents.
Topics: Animals; Carrier Proteins; Diethyl Pyrocarbonate; Dogs; Histidine; Hydroxylamine; Hydroxylamines; Kidney Cortex; Kinetics; Microvilli; Photochemistry; Rabbits; Rose Bengal; Sodium; Sodium-Hydrogen Exchangers; Time Factors | 1986 |
Chemical modification of human neutrophil membrane proteins: effect on fMet-Leu-Phe binding and function.
Topics: Diethyl Pyrocarbonate; Histidine; Humans; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Imidazoles; Membrane Proteins; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Protein Binding; Receptors, Formyl Peptide; Receptors, Immunologic; Superoxides | 1986 |
Metabolism of glycerate 2,3-P2--XI. Essential amino acids of pig phosphoglycerate mutase isozymes.
Topics: Animals; Diacetyl; Diethyl Pyrocarbonate; Hydroxylamine; Hydroxylamines; Isoenzymes; Kinetics; Phosphoglycerate Mutase; Phosphoric Monoester Hydrolases; Phosphotransferases; Pyruvaldehyde; Swine | 1987 |
Differential inhibition of estrogen and antiestrogen binding to the estrogen receptor by diethylpyrocarbonate.
Topics: Animals; Binding, Competitive; Cytosol; Diethyl Pyrocarbonate; Diethylstilbestrol; Estradiol; Estriol; Estrogen Antagonists; Female; Formates; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Molybdenum; Nitromifene; Receptors, Estrogen; Sheep; Tamoxifen; Uterus | 1988 |
Diethyl pyrocarbonate (an imidazole binding substance) inhibits rostral VLM CO2 sensitivity.
Topics: Animals; Carbon Dioxide; Cats; Chemoreceptor Cells; Diethyl Pyrocarbonate; Female; Formates; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Imidazoles; Male; Medulla Oblongata; Respiration | 1986 |
Rh antigen immunoreactivity after histidine modification.
Topics: Diethyl Pyrocarbonate; Erythrocyte Membrane; Histidine; Humans; Hydroxylamine; Hydroxylamines; Isoantigens; Oxidation-Reduction; Rh-Hr Blood-Group System; Rose Bengal | 1986 |
Chemical modification of acyl-CoA:cholesterol O-acyltransferase. 1. Identification of acyl-CoA:cholesterol O-acyltransferase subtypes by differential diethyl pyrocarbonate sensitivity.
Topics: Acetates; Acetic Anhydrides; Animals; Aorta; Binding Sites; Chemical Phenomena; Chemistry; Diethyl Pyrocarbonate; Formates; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Male; Microsomes; Microsomes, Liver; Rabbits; Sterol O-Acyltransferase; Tissue Distribution | 1988 |
A study of the B-Z transition of the AC-rich region of the repeat unit of a satellite DNA from Cebus by means of chemical probes.
Topics: Animals; Base Composition; Base Sequence; Cebus; Chemical Phenomena; Chemistry; Diethyl Pyrocarbonate; DNA, Satellite; Hydroxylamine; Hydroxylamines; Immunoassay; Molecular Sequence Data; Nucleic Acid Conformation; Osmium Tetroxide; Repetitive Sequences, Nucleic Acid; Sulfuric Acid Esters | 1988 |
Reaction of 5-enol-pyruvoylshikimate-3-phosphate synthase with diethyl pyrocarbonate: evidence for an essential histidine residue.
Topics: 3-Phosphoshikimate 1-Carboxyvinyltransferase; Alkyl and Aryl Transferases; Binding Sites; Catalysis; Chemical Phenomena; Chemistry; Diethyl Pyrocarbonate; Escherichia coli; Formates; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Spectrophotometry; Structure-Activity Relationship; Transferases | 1987 |
Arginyl and histidyl groups are essential for organic anion exchange in renal brush-border membrane vesicles.
Topics: Aldehydes; Animals; Arginine; Butanones; Diacetyl; Diethyl Pyrocarbonate; Dithiothreitol; Dogs; Formates; Histidine; Hydroxylamine; Hydroxylamines; Kidney Cortex; Kinetics; Microvilli; p-Aminohippuric Acid; Phenylglyoxal | 1988 |
Inactivation of the intestinal uptake system for beta-lactam antibiotics by diethylpyrocarbonate.
Topics: Animals; Anti-Bacterial Agents; Biological Transport; Cephalexin; Cephalosporins; Chemical Phenomena; Chemistry; Diethyl Pyrocarbonate; Formates; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Intestine, Small; Kinetics; Microvilli; Penicillins; Rabbits; Structure-Activity Relationship | 1988 |
Chemical modification of Escherichia coli RNA polymerase by diethyl pyrocarbonate: evidence of histidine requirement for enzyme activity and intrinsic zinc binding.
Topics: Diethyl Pyrocarbonate; DNA-Directed RNA Polymerases; Escherichia coli; Formates; Histidine; Hydroxylamine; Hydroxylamines; Kinetics; Macromolecular Substances; Protein Binding; Spectrophotometry; Zinc | 1986 |
Evidence for the involvement of histidine at the active site of glutathione S-transferase psi from human liver.
Topics: Binding Sites; Diethyl Pyrocarbonate; Glutathione; Glutathione Transferase; Histidine; Humans; Hydroxylamine; Hydroxylamines; In Vitro Techniques; Liver | 1987 |
Diethylpyrocarbonate inhibition of sodium-glucose cotransport in kidney brush-border membrane vesicles.
Topics: Animals; Diethyl Pyrocarbonate; Formates; Hydroxylamine; Hydroxylamines; Kidney Cortex; Kinetics; Microvilli; Monosaccharide Transport Proteins; Phlorhizin; Swine | 1987 |
Intracisternal diethylpyrocarbonate inhibits central chemosensitivity in conscious rabbits.
Topics: Animals; Chemoreceptor Cells; Cisterna Magna; Consciousness; Diethyl Pyrocarbonate; Formates; Hydroxylamine; Hydroxylamines; Hypoxia; Injections; Rabbits | 1986 |
Modification of bovine heart succinate dehydrogenase with ethoxyformic anhydride and rose bengal: evidence for essential histidyl residues protectable by substrates.
Topics: Animals; Cattle; Diethyl Pyrocarbonate; Formates; Histidine; Hydroxylamine; Hydroxylamines; Malonates; Mitochondria, Heart; Oxidation-Reduction; Photochemistry; Rose Bengal; Succinate Dehydrogenase | 1986 |
Acid-base catalysis in the argininosuccinate lyase reaction.
Topics: Animals; Argininosuccinate Lyase; Cattle; Diethyl Pyrocarbonate; Ethyldimethylaminopropyl Carbodiimide; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Liver; Lyases; Mathematics; Temperature | 1985 |
Diethylpyrocarbonate inactivation of NAD-malic enzyme from Ascaris suum.
Topics: Animals; Ascaris; Binding Sites; Chemical Phenomena; Chemistry; Diethyl Pyrocarbonate; Formates; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Malate Dehydrogenase; Malates; NAD; Spectrometry, Fluorescence | 1985 |
Chemical probes of DNA conformation: detection of Z-DNA at nucleotide resolution.
Topics: Base Sequence; Diethyl Pyrocarbonate; DNA; DNA, Superhelical; Hydroxylamine; Hydroxylamines; Nucleic Acid Conformation; Osmium Tetroxide; Plasmids; Purines; Pyrimidines | 1985 |
Reversible inhibition of the mitochondrial ubiquinol-cytochrome c oxidoreductase complex (complex III) by ethoxyformic anhydride.
Topics: Anaerobiosis; Antimycin A; Binding, Competitive; Cytochromes; Diethyl Pyrocarbonate; Electron Transport Complex III; Formates; Hydroxylamine; Hydroxylamines; Kinetics; Mitochondria; Multienzyme Complexes; NADH, NADPH Oxidoreductases; Quinone Reductases; Submitochondrial Particles | 1982 |
Inhibition of mitochondrial NADH:ubiquinone oxidoreductase by ethoxyformic anhydride.
Topics: Animals; Cattle; Diethyl Pyrocarbonate; Electron Transport; Formates; Histidine; Hydroxylamine; Hydroxylamines; Kinetics; Meperidine; Mitochondria, Heart; NAD; NAD(P)H Dehydrogenase (Quinone); NADH, NADPH Oxidoreductases; Quinone Reductases; Tyrosine | 1984 |
Chemical modification of histidine residues in rabbit liver glutathione reductase.
Topics: Animals; Dansyl Compounds; Diethyl Pyrocarbonate; Glutathione Reductase; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Liver; Rabbits; Spectrometry, Fluorescence; Structure-Activity Relationship | 1984 |
Involvement of some amino acid residues in the enzymatic activity of solubilized cerebral fucosyltransferase.
Topics: Animals; Arginine; Binding Sites; Brain; Butanones; Diacetyl; Diethyl Pyrocarbonate; Formates; Fucosyltransferases; Hexosyltransferases; Histidine; Hydroxylamine; Hydroxylamines; Kinetics; Methane; Sheep; Tetranitromethane; Tyrosine | 1984 |
Histidyl residues at the active site of the Na/succinate co-transporter in rabbit renal brush borders.
Topics: Animals; Binding Sites; Biological Transport; Carrier Proteins; Diethyl Pyrocarbonate; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kidney Cortex; Lactates; Lactic Acid; Lithium; Malates; Microvilli; Monosaccharide Transport Proteins; Rabbits; Sodium; Succinates; Succinic Acid | 1984 |
Diethylpyrocarbonate, a histidine selective reagent, inhibits progestin binding to chick oviduct cytosol.
Topics: Animals; Chemical Phenomena; Chemistry; Chickens; Cytosol; Diethyl Pyrocarbonate; Female; Formates; Histidine; Hydroxylamine; Hydroxylamines; In Vitro Techniques; Oviducts; Progestins; Promegestone; Receptors, Progesterone | 1983 |
Viroids and prions.
Topics: Diethyl Pyrocarbonate; Hydroxylamine; Hydroxylamines; Plant Viruses; Prions; RNA, Viral; Species Specificity; Viroids; Virus Replication | 1982 |
Diethylpyrocarbonate inhibition of estradiol receptor binding to oligonucleotides.
Topics: Amino Acids; Animals; Cattle; Diethyl Pyrocarbonate; Estradiol; Female; Formates; Histidine; Hydroxylamine; Hydroxylamines; Imidazoles; Oligonucleotides; Pyridoxal Phosphate; Receptors, Estradiol; Receptors, Estrogen; Uterus | 1981 |
Purification and characterization of allantoate amidohydrolase from Bacillus fastidiosus.
Topics: Allantoin; Bacillus; Diethyl Pyrocarbonate; Enzyme Activation; Guanidine; Guanidines; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Isoelectric Point; Kinetics; Protein Conformation; Protein Denaturation; Urea; Ureohydrolases | 1995 |
Single-channel currents from diethylpyrocarbonate-modified NMDA receptors in cultured rat brain cortical neurons.
Topics: Animals; Brain; Cells, Cultured; Diethyl Pyrocarbonate; Histidine; Hydroxylamine; Hydroxylamines; Ion Channel Gating; Ion Channels; Kinetics; Magnesium; Neurons; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; Receptors, N-Methyl-D-Aspartate; Zinc | 1995 |
Mutational analysis of transmembrane histidines in the amiloride-sensitive Na+/H+ exchanger.
Topics: Amiloride; Amino Acids; Animals; Base Sequence; Cell Line; Cimetidine; Diethyl Pyrocarbonate; Dose-Response Relationship, Drug; Histidine; Hydroxylamine; Hydroxylamines; Kinetics; Membranes; Mice; Molecular Probes; Molecular Sequence Data; Mutagenesis, Site-Directed; Sodium-Hydrogen Exchangers | 1995 |
Modulation of the mitochondrial cyclosporin A-sensitive permeability transition pore by matrix pH. Evidence that the pore open-closed probability is regulated by reversible histidine protonation.
Topics: Animals; Calcium; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; Cyclosporine; Diethyl Pyrocarbonate; Fluoresceins; Fluorescent Dyes; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Ion Channel Gating; Ion Channels; Kinetics; Mitochondria, Liver; Rats | 1993 |
Expression, purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from tomato in Escherichia coli.
Topics: Amino Acid Oxidoreductases; Base Sequence; Carbon Dioxide; Catalase; Cloning, Molecular; Diethyl Pyrocarbonate; Dithiothreitol; DNA, Complementary; Enzyme Induction; Escherichia coli; Histidine; Hydroxylamine; Hydroxylamines; Iron; Isopropyl Thiogalactoside; Molecular Sequence Data; Plant Proteins; Polymerase Chain Reaction; Recombinant Fusion Proteins; Serum Albumin, Bovine; Solanum lycopersicum; Stereoisomerism; Substrate Specificity; Transfection | 1995 |
Mechanistic studies on CDP-6-deoxy-L-threo-D-glycero-4-hexulose 3-dehydrase identification of His-220 as the active-site base by chemical modification and site-directed mutagenesis.
Topics: Amino Acid Sequence; Base Sequence; Binding Sites; Catalysis; Diethyl Pyrocarbonate; DNA Primers; Histidine; Hydro-Lyases; Hydroxylamine; Hydroxylamines; Molecular Sequence Data; Mutagenesis, Site-Directed; Sequence Alignment; Yersinia pseudotuberculosis | 1995 |
Inhibition of ligand binding to thromboxane A2/prostaglandin H2 receptors by diethylpyrocarbonate. Protection by receptor ligands and reversal by hydroxylamine.
Topics: Blood Platelets; Bridged Bicyclo Compounds; Bridged Bicyclo Compounds, Heterocyclic; Diethyl Pyrocarbonate; Fatty Acids, Monounsaturated; Fatty Acids, Unsaturated; Humans; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Radioligand Assay; Receptors, Prostaglandin; Receptors, Thromboxane; Receptors, Thromboxane A2, Prostaglandin H2 | 1995 |
Chemical modification of a cloned glutathione S-transferase from Schistosoma japonicum: evidence for an essential histidine residue.
Topics: Animals; Diethyl Pyrocarbonate; Glutathione; Glutathione Transferase; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Mutagenesis, Site-Directed; Recombinant Proteins; Schistosoma japonicum | 1995 |
Investigation of the active site of the cyanogenic beta-D-glucosidase (linamarase) from Manihot esculenta Crantz (cassava). I. Evidence for an essential carboxylate and a reactive histidine residue in a single catalytic center.
Topics: beta-Glucosidase; Binding Sites; Carbodiimides; Catalysis; Diethyl Pyrocarbonate; Histidine; Hydrogen-Ion Concentration; Hydrolysis; Hydroxylamine; Hydroxylamines; Isoxazoles; Kinetics; Manihot; Nitriles; Spectrophotometry; Substrate Specificity | 1994 |
Gradual and oriented B-Z transition in 5'-untranscribed region of mouse ribosomal DNA.
Topics: Acetaldehyde; Animals; Base Composition; Base Sequence; Diethyl Pyrocarbonate; DNA, Bacterial; DNA, Recombinant; DNA, Ribosomal; Electrophoresis, Gel, Two-Dimensional; Escherichia coli; Hydroxylamine; Hydroxylamines; Mice; Models, Molecular; Molecular Probes; Molecular Sequence Data; Nucleic Acid Conformation; Osmium Tetroxide; Repetitive Sequences, Nucleic Acid | 1994 |
Modification of pig kidney diamine oxidase with ethoxyformic anhydride and rose bengal: evidence for essential histidyl residue at the active site.
Topics: Amine Oxidase (Copper-Containing); Animals; Binding Sites; Diethyl Pyrocarbonate; Histidine; Hydroxylamine; Hydroxylamines; Kidney; Kinetics; Oxidants, Photochemical; Putrescine; Rose Bengal; Spectrometry, Fluorescence; Substrate Specificity; Swine | 1994 |
Metal-catalyzed oxidation of Fe2+ dehydrogenases. Consensus target sequence between propanediol oxidoreductase of Escherichia coli and alcohol dehydrogenase II of Zymomonas mobilis.
Topics: Alcohol Dehydrogenase; Amino Acid Sequence; Chromatography, Gel; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Consensus Sequence; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Escherichia coli; Hydroxylamine; Hydroxylamines; Iron; Isoenzymes; Molecular Sequence Data; Peptide Fragments; Sequence Homology, Amino Acid; Sugar Alcohol Dehydrogenases; Trinitrobenzenesulfonic Acid; Zymomonas | 1994 |
Chemical modification of human UDP-glucuronosyltransferase UGT1*6 by diethyl pyrocarbonate: possible involvement of a histidine residue in the catalytic process.
Topics: Affinity Labels; Animals; Catalysis; Cell Line; Cricetinae; Cricetulus; Diethyl Pyrocarbonate; Enzyme Activation; Glucuronates; Glucuronosyltransferase; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Hymecromone; Kinetics; Lung; Recombinant Proteins; Uridine Diphosphate Glucuronic Acid | 1994 |
Inhibitors of the proton-sucrose symport.
Topics: 4-Chloromercuribenzenesulfonate; Adenosine Triphosphate; Biological Transport; Carrier Proteins; Cell Membrane; Diethyl Pyrocarbonate; Energy Metabolism; Enzyme Reactivators; Freezing; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Membrane Transport Proteins; Mercury; Plant Proteins; Polyethylene Glycols; Protons; Sucrose; Vanadates; Vegetables | 1993 |
Inactivation of the endogenous argininosuccinate lyase activity of duck delta-crystallin by modification of an essential histidine residue with diethyl pyrocarbonate.
Topics: Animals; Arginine; Argininosuccinate Lyase; Crystallins; Diethyl Pyrocarbonate; Ducks; Fumarates; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Spectrometry, Fluorescence | 1993 |
Guanine nucleotide-specific phosphate transfer by guanine nucleotide-binding regulatory protein beta-subunits. Characterization of the phosphorylated amino acid.
Topics: Biological Transport; Cell Membrane; Diethyl Pyrocarbonate; Electrophoresis, Polyacrylamide Gel; GTP-Binding Proteins; Guanine Nucleotides; Guanosine 5'-O-(3-Thiotriphosphate); Guanosine Triphosphate; Histidine; Humans; Hydroxylamine; Hydroxylamines; Kinetics; Leukemia, Promyelocytic, Acute; Macromolecular Substances; Magnesium; Manganese; Membrane Proteins; Molecular Weight; Phosphorus Radioisotopes; Phosphorylation; Tumor Cells, Cultured | 1993 |
Site-directed mutagenesis and chemical modification of histidine residues on an alpha-class chick liver glutathione S-transferase CL 3-3. Histidines are not needed for the activity of the enzyme and diethylpyrocarbonate modifies both histidine and lysine
Topics: Amino Acid Sequence; Animals; Baculoviridae; Base Sequence; Chickens; Diethyl Pyrocarbonate; Glutathione Transferase; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Liver; Lysine; Molecular Sequence Data; Moths; Mutagenesis; Mutagenesis, Site-Directed; Peptide Mapping; Spectrophotometry; Trypsin | 1993 |
Folate transport in intestinal brush border membrane: involvement of essential histidine residue(s).
Topics: Animals; Binding Sites; Biological Transport; Carrier Proteins; Diethyl Pyrocarbonate; Folate Receptors, GPI-Anchored; Folic Acid; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Intestinal Mucosa; Intestines; Kinetics; Microvilli; Rabbits; Receptors, Cell Surface | 1993 |
Arginine residues of the globular regions of human C1q involved in the interaction with immunoglobulin G.
Topics: Amino Acid Sequence; Animals; Antigen-Antibody Complex; Arginine; Binding Sites, Antibody; Chromatography, High Pressure Liquid; Complement C1q; Cyclohexanones; Diethyl Pyrocarbonate; Histidine; Humans; Hydroxylamine; Hydroxylamines; Immunoglobulin G; Kinetics; Macromolecular Substances; Molecular Sequence Data; Peptide Fragments; Phenylglyoxal; Protein Conformation; Rabbits | 1993 |
Chemical modification of porcine kidney aminopeptidase P indicates the involvement of two critical histidine residues.
Topics: Aminopeptidases; Animals; Binding Sites; Bradykinin; Cell Membrane; Diethyl Pyrocarbonate; Enzyme Inhibitors; Histidine; Hydroxylamine; Hydroxylamines; Kidney; Kinetics; Oligopeptides; Peptides; Spectrophotometry, Ultraviolet; Swine | 1996 |
Rabbit muscle creatine kinase: consequences of the mutagenesis of conserved histidine residues.
Topics: Amino Acid Sequence; Animals; Cloning, Molecular; Conserved Sequence; Creatine Kinase; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Escherichia coli; Histidine; Hot Temperature; Hydroxylamine; Hydroxylamines; Kinetics; Molecular Sequence Data; Muscle, Skeletal; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Rabbits; Recombinant Proteins; Sequence Homology, Amino Acid; Thermodynamics | 1996 |
The reaction of phosphoglycerate mutase from Schizosaccharomyces pombe with diethylpyrocarbonate.
Topics: Binding Sites; Circular Dichroism; Diethyl Pyrocarbonate; Glutamine; Histidine; Hydroxylamine; Hydroxylamines; Kinetics; Mutagenesis, Site-Directed; Phosphoglycerate Mutase; Protein Conformation; Recombinant Proteins; Schizosaccharomyces; Spectrometry, Fluorescence | 1996 |
Evidence for the essential histidine at the NADPH binding site of enoyl-CoA reductase domain of pigeon liver fatty acid synthetase.
Topics: Animals; Binding Sites; Columbidae; Diethyl Pyrocarbonate; Enoyl-(Acyl-Carrier-Protein) Reductase (NADH); Enzyme Activation; Fatty Acid Synthases; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Liver; NADP; Oxidoreductases; Protein Structure, Tertiary; Substrate Specificity; Time Factors | 1997 |
Vitamin K2 (menaquinone) biosynthesis in Escherichia coli: evidence for the presence of an essential histidine residue in o-succinylbenzoyl coenzyme A synthetase.
Topics: Adenosine Triphosphate; Binding Sites; Diethyl Pyrocarbonate; Escherichia coli; Histidine; Hydroxylamine; Hydroxylamines; Kinetics; Magnesium; Mutagenesis, Site-Directed; Phenylbutyrates; Spectrophotometry, Ultraviolet; Succinate-CoA Ligases; Vitamin K | 1997 |
Evidence for the essential histidine residues in geranylgeranyl transferase type I from bovine testis.
Topics: Alkyl and Aryl Transferases; Animals; Binding Sites; Cattle; Chromatography; Diethyl Pyrocarbonate; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Iodoacetates; Iodoacetic Acid; Kinetics; Male; Testis | 1997 |
Diethyl pyrocarbonate (DEPC) inhibits CO2 chemosensitivity in Helix aspersa.
Topics: Ammonium Chloride; Animals; Carbon Dioxide; Chemoreceptor Cells; Diethyl Pyrocarbonate; Helix, Snails; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hypercapnia; Hypoxia; In Vitro Techniques; Nitroprusside; Respiration | 1998 |
Purification and some molecular properties of pigeon heart AMP-selective 5'-nucleotidase.
Topics: 5'-Nucleotidase; Adenosine; Adenosine Monophosphate; Animals; Chromatography, Affinity; Columbidae; Cytosol; Diethyl Pyrocarbonate; Hydroxylamine; Isoelectric Focusing; Kinetics; Myocardium; Substrate Specificity | 1998 |
Potential role for imidazole in the rhythmic respiratory activity of the in vitro neonatal rat brainstem.
Topics: Animals; Animals, Newborn; Brain Stem; Diethyl Pyrocarbonate; Histidine; Hydroxylamine; Imidazoles; In Vitro Techniques; Medulla Oblongata; Microinjections; Periodicity; Rats; Rats, Sprague-Dawley; Respiration; Spinal Cord | 1998 |
Localization of critical histidyl residues required for vinblastine-induced tubulin polymerization and for microtubule assembly.
Topics: Animals; Diethyl Pyrocarbonate; Histidine; Hydroxylamine; Microtubules; Peptide Mapping; Polymers; Rats; Tubulin; Vinblastine | 1998 |
Essential histidyl residues at the active site(s) of sucrose-phosphate synthase from Prosopis juliflora.
Topics: Amino Acid Sequence; Binding Sites; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Enzyme Inhibitors; Fructosephosphates; Glucosyltransferases; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Kinetics; Molecular Sequence Data; Oxidation-Reduction; Plant Proteins; Plants; Pyridoxal Phosphate; Rose Bengal; Spectrophotometry; Uridine Diphosphate Glucose | 1998 |
Kinetic and mutagenic evidence for the role of histidine residues in the Lycopersicon esculentum 1-aminocyclopropane-1-carboxylic acid oxidase.
Topics: Amino Acids; Amino Acids, Cyclic; Circular Dichroism; Cloning, Molecular; Deoxycholic Acid; Diethyl Pyrocarbonate; Ethylenes; Guanidine; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Imidazoles; Inclusion Bodies; Kinetics; Mutagenesis, Site-Directed; Recombinant Proteins; Solanum lycopersicum; Spectrometry, Fluorescence; Time Factors | 1999 |
Chemical modification of NADP-isocitrate dehydrogenase from Cephalosporium acremonium evidence of essential histidine and lysine groups at the active site.
Topics: Acremonium; Binding Sites; Diethyl Pyrocarbonate; Enzyme Inhibitors; Enzyme Reactivators; Histidine; Hydroxylamine; Isocitrate Dehydrogenase; Kinetics; Lysine; Pyridoxal Phosphate | 1999 |
Inhibition of mitochondrial permeability transition by low pH is associated with less extensive membrane protein thiol oxidation.
Topics: Animals; Antimycin A; Arsenicals; Calcimycin; Calcium; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; Diethyl Pyrocarbonate; Hydrogen-Ion Concentration; Hydroxylamine; Intracellular Membranes; Ionophores; Membrane Proteins; Mitochondria, Liver; Oxidation-Reduction; Permeability; Rats; Rats, Wistar; Serum Albumin, Bovine; Sulfhydryl Compounds | 1999 |
Diethylpyrocarbonate inhibition of vacuolar H+-pyrophosphatase possibly involves a histidine residue.
Topics: Diethyl Pyrocarbonate; Enzyme Inhibitors; Fabaceae; Histidine; Hydroxylamine; Inorganic Pyrophosphatase; Ligands; Plant Proteins; Protons; Pyrophosphatases; Regression Analysis; Vacuoles | 2002 |
Enterococcus faecalis acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase, a dual-function protein of isopentenyl diphosphate biosynthesis.
Topics: Acyl Coenzyme A; Amino Acid Sequence; Diethyl Pyrocarbonate; Enterococcus faecalis; Hemiterpenes; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxymethylglutaryl CoA Reductases; Kinetics; Molecular Sequence Data; Organophosphorus Compounds; Substrate Specificity; Temperature | 2002 |
Insights into the interaction of human liver arginase with tightly and weakly bound manganese ions by chemical modification and site-directed mutagenesis studies.
Topics: Arginase; Arginine; Diethyl Pyrocarbonate; Enzyme Activation; Histidine; Humans; Hydroxylamine; Liver; Manganese; Mutagenesis, Site-Directed; Point Mutation | 2002 |
Probing the role of active site histidine residues in the catalytic activity of lacrimal gland peroxidase.
Topics: Animals; Binding Sites; Catalysis; Circular Dichroism; Diethyl Pyrocarbonate; Dose-Response Relationship, Drug; Heme; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Kinetics; Lacrimal Apparatus; Models, Chemical; Peroxidase; Protein Binding; Protein Structure, Secondary; Sheep; Substrate Specificity; Temperature; Time Factors; Ultraviolet Rays | 2002 |
Chemical modification of contractile 3-nm-diameter filaments in Vorticella spasmoneme by diethyl-pyrocarbonate and its reversible renaturation by hydroxylamine.
Topics: Animals; Ciliophora; Contractile Proteins; Diethyl Pyrocarbonate; Hydroxylamine; Protein Renaturation; Protozoan Proteins | 2003 |
Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver.
Topics: Amino Acid Sequence; Animals; Benzophenones; Cattle; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Enzyme Inhibitors; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Inhibitory Concentration 50; Intramolecular Transferases; Kinetics; Mass Spectrometry; Microsomes, Liver; Molecular Sequence Data; Peptide Fragments; Sequence Analysis, Protein; Sequence Homology, Amino Acid | 2004 |
Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase.
Topics: Asparagine; Binding Sites; Carboxylic Ester Hydrolases; Catalysis; Diethyl Pyrocarbonate; Escherichia coli Proteins; Fluorescent Dyes; Fluorometry; Histidine; Hydrogen-Ion Concentration; Hydrolysis; Hydroxylamine; Kinetics; Mutagenesis, Site-Directed; Phosphorylcholine; Point Mutation; Substrate Specificity | 2004 |
Evidence for the presence of a critical histidine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cells.
Topics: Animals; Carcinoma, Ehrlich Tumor; Catalytic Domain; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Gene Expression Regulation, Enzymologic; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Kinetics; Mice | 2004 |