hydrin-2 and mesotocin

From the pituitary neurointermediate lobe of the African toad Bufo regularis, vasotocin, hydrin 2 (vasotocinyl-Gly) and a mesotocin-like peptide have been isolated by HPLC and characterized by mass spectrometry, amino acid sequence and chromatographic coelution with synthetic peptides. The mesotocin-like peptide has been identified as [Ser5,Ile8]-oxytocin in place of mesotocin ([Ile8]-oxytocin) found in all other amphibians investigated to date. The name seritocin is suggested. The molecule is virtually devoid of oxytocic activity on rat uterus in contrast to mesotocin. On the other hand, the molar ratio of hydrin 2 to vasotocin in the pituitary reaches 2, whereas it is about 1 in toads and frogs from temperate regions. B. regularis is an anuran species able to withstand a hot and dry season by burrowing. The possible relationship between occurrence of seritocin and adaptation to arid environment remains to be demonstrated.

Topics: Amino Acid Sequence; Animals; Bufonidae; Chromatography, High Pressure Liquid; Environment; Molecular Sequence Data; Oxytocin; Sequence Analysis; Sequence Homology, Amino Acid; Vasotocin

Topics: Animals; Corticotropin-Releasing Hormone; Dopamine; Immunoblotting; In Vitro Techniques; Oxytocin; Peptide Fragments; Pituitary Gland; Pro-Opiomelanocortin; Rana catesbeiana; Rana esculenta; Thyrotropin-Releasing Hormone; Vasotocin

Hydrin 2 (vasotocinyl-Gly), a hydroosmotic peptide resulting from differential processing of provasotocin and recently identified in frog neurohypophysis, has been looked for in the pituitary gland of an exotic toad (Bufo marinus) and of a reptile (Vipera aspis). Hydrin 2 has been found in the amphibian but not in the reptile. This result confirms the evolutionary specificity of hydrin 2 that has been identified only in frogs and toads but not in birds and reptiles. Occurrence of hydrin 2 is explained by its regulatory function on the water permeability of the skin of anurans.

Topics: Animals; Biological Evolution; Bufo marinus; Chromatography, High Pressure Liquid; Neuropeptides; Oxytocin; Pituitary Gland; Snakes; Species Specificity; Vasotocin

From neurointermediate pituitary glands of Xenopus laevis and Rana esculenta, previously unreported peptides termed hydrins, active on water permeability of frog urinary bladder and frog skin (Brunn or "water-balance" effect), have been isolated and sequenced. These peptides seem to be derived from the pro-vasotocin-neurophysin precursor. Hydrin 1, found in Xenopus, has been identified as vasotocin C-terminally extended with the Gly-Lys-Arg sequence; hydrin 2, found in Rana, has been identified as vasotocin C-terminally extended with glycine. Hydrin 2 has been detected in several Ranidae (R. esculenta, Rana temporaria, Rana pipiens) and Bufonidae (Bufo bufo, Bufo ictericus) and appears to have a large distribution in terrestrial or semiaquatic anurans. Hydrins, in contrast to vasotocin, are not active on rat uterus or rat blood pressure. They are absent from other vasotocin-bearers such as birds and could be involved specifically in water-electrolyte regulation of amphibians.

Topics: Acclimatization; Amino Acid Sequence; Animals; Molecular Sequence Data; Oxytocin; Pituitary Gland, Posterior; Protein Processing, Post-Translational; Rana esculenta; Species Specificity; Vasotocin; Water-Electrolyte Balance; Xenopus laevis