humulene and terpinolene

In 2019, an air emissions field sampling study was conducted by the Colorado Department of Public Health and Environment's Air Pollution Control Division (APCD) at four commercial cannabis cultivation facilities. Measurements of ambient biogenic volatile organic compounds (VOC) concentrations were collected from various growing stages of cannabis (vegetative and flowering) and during post-harvest activities (drying and trimming). These data were then used to determine room-specific biogenic VOC emission rates for three of the facilities from the vegetative stage of the life cycle through post-harvest activities. This study shows that the magnitude of biogenic VOC emissions within a cannabis cultivation facility varies widely with the highest emission rates of up to 7.18E-1 kg/hr found during mechanical trimming and up to 2.33E-1 kg/hr in the drying rooms. These were up to an order of magnitude higher than emission rates found in the cultivation rooms. For example, Facility A vegetative room had an emissions rate of 1.46E-2 kg/hr. Normalized by the amount of biomass present, the drying rooms had the highest VOC emissions rates, with a maximum rate of 1.6E-3 kg/hr/kg biomass. The flowering room rates were found to be up to 3.25E-4 kg/hr/kg biomass and drying rooms up to 1.16E-3 kg/hr/kg biomass. When normalized by plant count, emission rates in the flower rooms ranged from 8.11E-6 to 3.62E-4 kg/hr/plant. The dominant monoterpenes from sampling were β-myrcene, terpinolene, and D-limonene. These data suggest that the variability in emission rates across cannabis production will create a challenge in establishing a generalized emission factor for all facilities. Across the industry, cannabis cultivation conditions and strategies can vary widely impacting the amount and type of VOC emissions. Minimizing uncertainties for VOC emission from cannabis facilities requires site-specific information on air exchange rates, plant counts, cannabis strains, biomass, and if hand or mechanical processing is used.

Topics: Air Pollutants; Cannabis; Colorado; Environmental Monitoring; Limonene; Monoterpenes; Volatile Organic Compounds

The hydro-distilled essential oil from aerial parts of

Topics: Bicyclic Monoterpenes; Cyclohexane Monoterpenes; Gas Chromatography-Mass Spectrometry; Lamiaceae; Monocyclic Sesquiterpenes; Oils, Volatile; Orthosiphon; Polycyclic Sesquiterpenes; Sesquiterpenes

Dormant buds are recognized as valuable side product of the blackcurrant cultivation. Four blackcurrant varieties cultivated in Serbia, i.e., Ben Sarek, Ometa, Ben Lomond, and Ben Nevis, were evaluated for the content, chemical composition, and antimicrobial activity of their bud essential oils. The oil yields of buds harvested during two different growth periods ranged from 1.2-2.0%, and the variety Ometa had the highest yield among the tested varieties. GC-FID and GC/MS analysis of the oils allowed the identification of eight main components, i.e., α-pinene (1.6-5.4%), sabinene (1.9-38.4%), δ-car-3-ene (13.0-50.7%), β-phellandrene (2.9-18.0%), terpinolene (6.6-11.9%), terpinen-4-ol (0.9-6.6%), β-caryophyllene (3.8-10.4%), and α-humulene (0.2-4.1%). In addition, the similarity degree of the essential-oil compositions of buds harvested from the upper and lower parts of the shrubs was investigated by hierarchical clustering. All essential oils originating from the same genotype were grouped in the same cluster, indicating the reliability of essential oils as chemotaxonomic markers. For more detailed chemotaxonomic investigations, the three compounds with the greatest variance were chosen, i.e., sabinene, δ-car-3-ene, and β-phellandrene, which proved to be efficient for the variety distinction. Factor analysis showed that the essential-oil composition as chemotaxonomic marker in blackcurrants was more reliable for variety Ben Sarek than for variety Ben Nevis. Moreover, it was demonstrated that the essential oils had very strong inhibitory activity against all tested microorganisms. Fungi were more sensitive than bacteria; indeed their growth was completely inhibited at much lower concentrations. In comparison to commercial antibiotics, significantly lower concentrations of the oils were necessary for the complete inhibition of fungal growth.

Topics: Anti-Infective Agents; Bicyclic Monoterpenes; Biomarkers; Cyclohexane Monoterpenes; Cyclohexenes; Drug Resistance, Bacterial; Gas Chromatography-Mass Spectrometry; Microbial Sensitivity Tests; Molecular Typing; Monocyclic Sesquiterpenes; Monoterpenes; Oils, Volatile; Polycyclic Sesquiterpenes; Principal Component Analysis; Ribes; Serbia; Sesquiterpenes; Terpenes