ht-2-toxin has been researched along with tri-o-cresyl-phosphate* in 1 studies
1 other study(ies) available for ht-2-toxin and tri-o-cresyl-phosphate
| Article | Year |
|---|---|
Metabolism of T-2 toxin by rat liver carboxylesterase.
The trichothecene T-2 toxin was rapidly hydrolyzed by rat liver microsomal fraction into HT-2 toxin which was the main metabolite. The metabolism was completely blocked by paraoxon, a serine esterase inhibitor, but not affected by EDTA or 4-hydroxy mercury benzoate, inhibitors of arylesterase and esterases containing SH-group in active site, respectively. Among the serine esterases carboxylesterase (EC 3.1.1.1), but not cholinesterase (EC 3.1.1.8) hydrolysed T-2 toxin to HT-2 toxin. Carboxylesterase activity from liver microsomes was separated into at least five different isoenzymes by isoelectric focusing, and only the isoenzyme of pI 5.4 was able to hydrolyse T-2 toxin to HT-2 toxin. The toxicity of T-2 toxin in mice was enhanced by pre-treatment with tri-o-cresyl phosphate (TOCP), a specific carboxylesterase inhibitor. This confirms the importance of carboxylesterase in detoxification of trichothecenes. Topics: Animals; Carboxylesterase; Carboxylic Ester Hydrolases; Isoelectric Focusing; Isoenzymes; Male; Microsomes, Liver; Rats; Rats, Inbred Strains; Sesquiterpenes; T-2 Toxin; Tritolyl Phosphates | 1986 |