histidine has been researched along with sepharose in 63 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 7 (11.11) | 18.7374 |
| 1990's | 18 (28.57) | 18.2507 |
| 2000's | 20 (31.75) | 29.6817 |
| 2010's | 13 (20.63) | 24.3611 |
| 2020's | 5 (7.94) | 2.80 |
| Authors | Studies |
|---|---|
| Schmer, G; Thaler, E | 1 |
| Feeney, RE; Ryan, DS | 1 |
| Hiwada, K; Kokubu, T; Nishimura, K; Ueda, E | 1 |
| Schweri, MM; Wacker, WB; Yankeelov, JA | 1 |
| el-Kak, A; Vijayalakshmi, MA | 1 |
| Barnett, T; Drake, L | 1 |
| Abdul Nour, J; Birkenmeir, G; Goubran-Botros, H; Nanak, E; Vijayalakshmi, MA | 1 |
| Aulak, KS; Harrison, RS | 1 |
| De Wolf, M; Dierick, W; Hilderson, H; Lagrou, A; Van Dessel, G; Verhaeghe, M | 1 |
| Amourache, L; Kanoun, S; Krishnan, S; Vijayalakshmi, MA | 1 |
| Ishii, S; Kasai, K; Nishikata, M | 1 |
| Hunt, T; Poon, RY | 1 |
| Mandjiny, S; Vijayalakshmi, MA | 1 |
| Balasubramanian, AS; Bhanumathy, CD | 1 |
| Müller, HN; Schmidt, AM; Skerra, A | 1 |
| Abrams, WR; Czerwinski, M; Siegel, DL; Spitalnik, SL; Stwora-Wojczyk, MM; Wojczyk, BS; Wunner, WH | 1 |
| Anspach, FB; Deckwer, WD; Petsch, D | 1 |
| Deutscher, SL; Komissarov, AA; Marchbank, MT | 1 |
| Dunnill, P; O'Brien, SM; Sloane, RP; Thomas, OR | 1 |
| Hooper, NM; Keynan, S; Turner, AJ | 1 |
| Arndt, KM; Bauer, K; Müller, KM; Plückthun, A | 1 |
| Baron, T; Betemps, D; Cheynet, V; Mallet, F | 1 |
| Lee, SY; Xu, Z | 1 |
| Bochkariov, DE; Chaga, G; Hopp, J; Jokhadze, GG; Nelson, P | 1 |
| Daabrowski, S; Kur, J; Maciuńska, J; Sobiewska, G; Synowiecki, J | 1 |
| Cullinane, AM; Goad, JL; Horne, MK; Merryman, PK | 1 |
| Jeltsch, A; Lanio, T; Pingoud, A | 1 |
| Frank, S; Hrzenjak, A; Kostner, GM; Maderegger, B; Sterk, H | 1 |
| Mori, S; Nishibori, M; Okamoto, M; Yamaoka, K | 1 |
| Davies, N; Grant, A; O'Connor, CD; Owens, RM | 1 |
| Blum, LJ; Marquette, CA; Pizzolato, F; Tsafack, VC | 1 |
| Miron, T; Wilchek, M | 1 |
| Abedi, K; Alfaro, J; Badali, O; Barajas, M; Gipson, S; Harieg, CB; Khatibi, D; Khurrum, MR; Kirszenbaum, A; Kirszenbaum, L; Medina, J; Navarro, VM; Ngo, L; Oppenheimer, SB; Razi, A; Riman, R; Sakhakorn, T; Soriano, ES; Weerasinghe, GR; Zem, GC | 1 |
| Unrau, PJ; Wang, QS | 1 |
| Kashlev, M; Kireeva, ML; Komissarova, N; Lubkowska, L | 1 |
| Blackbourn, DJ; Blom, AM; Lee, WH; Mark, L; Proctor, D; Spiller, OB; Villoutreix, BO | 1 |
| Banerjee, AL; Mallik, S; Srivastava, DK; Swanson, M | 1 |
| Bartlett, RK; Bass, MA; Carmody, LC; Colbran, RJ; Jiao, Y; MacMillan, LB; Robison, AJ | 1 |
| Hjérten, S; Johansson, KE; Wróblewski, H | 1 |
| He, M; Khan, F; Taussig, MJ | 1 |
| Chen, C; Hua, ZC; Huang, QL; Jiang, SH; Pan, X | 1 |
| Prazeres, DM; Queiroz, JA; Sousa, F | 1 |
| Darby, RA; Forde, GM; Hine, AV; Slater, NK | 1 |
| Chen, C; Cui, YL; Hu, DD; Li, SJ; Sun, YL | 1 |
| Batista-Viera, F; Giacomini, C; Irazoqui, G; Puhl, AC; Terenzi, H; Villarino, A | 1 |
| Asher, WB; Bren, KL | 1 |
| Feng, J; Fu, LX; Li, JH; Qiao, YT; Sun, PC; Wang, J; Wang, W; Yuan, Z | 1 |
| Cabrita, EJ; Cruz, C; Queiroz, JA | 1 |
| Berenguer, J; Bolivar, JM; Guisán, JM; Hidalgo, A; López-Gallego, F; Sánchez-Ruiloba, L | 1 |
| Cabrita, EJ; Cruz, C; Queiroz, JA; Santos, SD | 1 |
| Boeuf, G; Bouallagui, H; Di Martino, P; Dridi, C; Dufour, F; El Marjou, A; Elm'selmi, A; Miladi, B | 1 |
| Arikawa, S; Yamada, S | 1 |
| Dong, XY; Du, WJ; Liu, H; Sun, Y | 1 |
| Assem, E; Edmond, C; Hiam, KM; Zeinab, M | 1 |
| de Tombe, PP; Khairallah, RJ; Kumar, M; Martin, JL; Zhang, M | 1 |
| Dong, X; Liu, H; Sun, Y | 1 |
| Cao, H; Han, L; Huang, C; Jia, L; Wang, R; Xu, L; Xu, T | 1 |
| Benítez-Mateos, AI; López-Gallego, F; Mehravar, E; Salassa, L; Velasco-Lozano, S | 1 |
| Crommen, J; Han, H; Huang, H; Huang, S; Jiang, Z; Lei, Y; Liu, X; Lu, L; Sun, L; Wang, Q; Xu, R | 1 |
| Kitagawa, T; Nagai, M; Nagatomo, S | 1 |
| Pavase, TR; Wang, C; Xue, C; Zheng, H | 1 |
| Tian, J; Wang, C; Wei, F; Xue, C; Zheng, H | 1 |
| Chen, MY; Dong, LY; Shi, YJ; Wang, XH; Wang, XY; Wang, Y; Yu, SS; Zhao, JM; Zhao, YQ; Zhao, ZY | 1 |
63 other study(ies) available for histidine and sepharose
| Article | Year |
|---|---|
A simple two-step isolation procedure for human and bovine antithrombin II/III (heparin cofactor): a comparison of two methods.
Topics: Alpha-Globulins; Animals; Antithrombin III; Cattle; Chromatography, Affinity; Chromatography, Gel; Drug Stability; Ethylene Glycols; Gels; Heparin; Histidine; Humans; Sepharose | 1975 |
The interaction of inhibitors of proteolytic enzymes with 3-methylhistidine-57-chymotrypsin.
Topics: Animals; Benzenesulfonates; Benzyl Compounds; Binding Sites; Binding, Competitive; Cattle; Chromatography, Affinity; Chymotrypsin; Ducks; Egg Proteins; Electrophoresis, Starch Gel; Histidine; Kinetics; Mathematics; Mesylates; Methylation; Poultry; Protein Binding; Sepharose; Time Factors; Turkeys | 1975 |
Affinity chromatography of angiotensin I-converting enzyme from rabbit lung using hippurylhistidylleucyl-OH.
Topics: Angiotensin II; Animals; Chromatography, Affinity; Hippurates; Histidine; Leucine; Lung; Oligopeptides; Peptidyl-Dipeptidase A; Rabbits; Sepharose | 1976 |
Radial diffusion assay of tissue collagenase and its application in evaluation of collagenase inhibitors.
Topics: Animals; Anura; Cysteine; Edetic Acid; Fishes; Histidine; Kinetics; Lathyrism; Methods; Microbial Collagenase; Rats; Sepharose; Skin | 1977 |
Separation and purification of IgG1 and IgG2 from IgG-Cohn-II fraction of human plasma by pseudobioaffinity chromatography on histidyl-AH-sepharose.
Topics: Chromatography, Affinity; Electrophoresis, Agar Gel; Histidine; Humans; Immunodiffusion; Immunoelectrophoresis; Immunoglobulin G; Isoelectric Point; Sensitivity and Specificity; Sepharose | 1992 |
A useful modification of cDNA that enhances purification of recombinant protein.
Topics: Base Sequence; Chromatography, Affinity; Codon; DNA, Recombinant; Histidine; Imino Acids; Molecular Sequence Data; Polymerase Chain Reaction; Recombinant Proteins; Sepharose | 1992 |
Immobilized metal ion affinity electrophoresis. A study with several model proteins containing histidine.
Topics: Electrophoresis, Agar Gel; Histidine; Ligands; Metals; Models, Chemical; Proteins; Sepharose | 1992 |
Rapid and sensitive techniques for identification and analysis of 'reactive-centre' mutants of C1-inhibitor proteins contained in type II hereditary angio-oedema plasmas.
Topics: Angioedema; Codon; Complement C1 Inactivator Proteins; Cysteine; Histidine; Humans; Mutation; Peptide Fragments; Sepharose; Trypsin | 1990 |
Identification of essential amino acids in the active center of thyroidal NAD+ glycohydrolase.
Topics: Amino Acids; Animals; Binding Sites; Cattle; Chemical Phenomena; Chemistry; Chromatography, Affinity; Coloring Agents; Histidine; Indicators and Reagents; N-Glycosyl Hydrolases; NAD+ Nucleosidase; Sepharose; Thyroid Gland | 1990 |
New support for the large-scale purification of proteins.
Topics: Animals; Carboxypeptidases; Cathepsin A; Cattle; Chick Embryo; Chymosin; Endopeptidases; Female; Histidine; Humans; Immunoelectrophoresis; Immunoglobulin G; Peptides; Placenta; Pregnancy; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sepharose; Serine Endopeptidases; Spectrophotometry, Ultraviolet | 1986 |
A sepharose derivative coupled with a leupeptin-like peptide aldehyde, glycylglycyl-L-argininal, and its use as an affinity adsorbent for trypsin.
Topics: Adsorption; Chromatography, Affinity; Histidine; Hydrogen-Ion Concentration; Leupeptins; Oligopeptides; Sepharose; Serine; Streptomyces griseus; Trypsin | 1981 |
Reversible immunoprecipitation using histidine- or glutathione S-transferase-tagged staphylococcal protein A.
Topics: Amino Acid Sequence; Bacteriophage T7; Base Sequence; Cloning, Molecular; Codon; Genetic Code; Glutathione Transferase; Histidine; Molecular Sequence Data; Phosphorylation; Precipitin Tests; Promoter Regions, Genetic; Protein Binding; Protein Structure, Tertiary; Recombinant Proteins; Sepharose; Staphylococcal Protein A | 1994 |
Quantitation of adsorption capacity of immunoglobulin G on histidine-aminohexyl sepharose and determination of affinity constant.
Topics: Adsorption; Chemical Phenomena; Chemistry, Physical; Chromatography, Affinity; Histidine; Humans; Immunoglobulin G; Ligands; Sepharose | 1993 |
Evidence for a Zn(2+)-binding site in human serum butyrylcholinesterase.
Topics: Binding Sites; Butyrylcholinesterase; Carboxypeptidases; Chelating Agents; Chromatography, Affinity; Edetic Acid; Histidine; Humans; Sepharose; Zinc | 1996 |
A Zn(II)-binding site engineered into retinol-binding protein exhibits metal-ion specificity and allows highly efficient affinity purification with a newly designed metal ligand.
Topics: Binding Sites; Carbonic Anhydrases; Chromatography, Affinity; Escherichia coli; Histidine; Humans; Imino Acids; In Vitro Techniques; Ligands; Models, Molecular; Molecular Structure; Protein Conformation; Protein Engineering; Recombinant Fusion Proteins; Retinol-Binding Proteins; Sepharose; Zinc | 1996 |
Purification of a secreted form of recombinant rabies virus glycoprotein: comparison of two affinity tags.
Topics: Affinity Labels; Amino Acid Sequence; Animals; Antigens, Viral; Bacterial Proteins; Base Sequence; Biotin; Blotting, Western; CHO Cells; Chromatography, Affinity; Cricetinae; Electrophoresis, Polyacrylamide Gel; Epitopes; Gene Expression Regulation; Glycoproteins; Histidine; Molecular Sequence Data; Recombinant Fusion Proteins; Sepharose; Sequence Analysis; Streptavidin; Transfection; Viral Envelope Proteins | 1996 |
Purification of murine IgG1 on group specific affinity sorbents.
Topics: Animals; Biotechnology; Cell Line; Chelating Agents; Chromatography, Affinity; Drug Contamination; Evaluation Studies as Topic; Histidine; Immunoglobulin G; Kinetics; Ligands; Mice; Polymers; Pyrogens; Sepharose; Thermodynamics | 1996 |
The use of Ni-nitrilotriacetic acid agarose for estimation of affinities of hexahistidine-tagged Fab to single-stranded DNA.
Topics: Affinity Labels; Animals; Chelating Agents; DNA, Single-Stranded; Histidine; Immunoglobulin Fab Fragments; Immunoglobulin Variable Region; In Vitro Techniques; Kinetics; Mice; Mutation; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Sepharose | 1997 |
Characterisation of non-porous magnetic chelator supports and their use to recover polyhistidine-tailed T4 lysozyme from a crude E. coli extract.
Topics: Bacteriophage T4; Bioreactors; Chelating Agents; Chromatography, Affinity; Copper; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Histidine; Magnetics; Muramidase; Peptides; Protein Binding; Recombinant Fusion Proteins; Sepharose | 1997 |
Identification by site-directed mutagenesis of three essential histidine residues in membrane dipeptidase, a novel mammalian zinc peptidase.
Topics: Amino Acid Sequence; Animals; Cell Membrane; Chlorocebus aethiops; Chromatography, Affinity; Cilastatin; COS Cells; Dipeptidases; Histidine; Humans; Kidney Cortex; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Sepharose; Swine | 1997 |
Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins--evaluation of two anti-His-tag monoclonal antibodies.
Topics: Amino Acid Sequence; Animals; Antibodies, Monoclonal; Chromatography, Affinity; Edetic Acid; Histidine; Immunoglobulin G; Immunoglobulin Isotypes; Indicators and Reagents; Kinetics; Metals; Mice; Molecular Sequence Data; Molecular Weight; Nickel; Recombinant Proteins; Sepharose; Staphylococcal Protein A | 1998 |
Overexpression and purification of an immunologically reactive His-BIV capsid fusion protein.
Topics: Animals; Antibodies, Monoclonal; Capsid; Cattle; Chromatography, Affinity; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gene Products, gag; Histidine; Immunodeficiency Virus, Bovine; Isopropyl Thiogalactoside; Open Reading Frames; Polymerase Chain Reaction; Recombinant Fusion Proteins; Sepharose | 1999 |
Display of polyhistidine peptides on the Escherichia coli cell surface by using outer membrane protein C as an anchoring motif.
Topics: Adsorption; Amino Acid Sequence; Bacterial Adhesion; Base Sequence; Binding Sites; Cadmium; Cell Membrane; Escherichia coli; Histidine; Models, Molecular; Molecular Sequence Data; Peptide Library; Peptides; Porins; Protein Structure, Secondary; Recombinant Proteins; Restriction Mapping; Sepharose | 1999 |
Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agarose.
Topics: Affinity Labels; Amino Acid Sequence; Aspartic Acid; Base Sequence; Chloramphenicol O-Acetyltransferase; Chromatography, Affinity; Cloning, Molecular; Cross-Linking Reagents; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Genetic Vectors; Green Fluorescent Proteins; Histidine; Luminescent Proteins; Molecular Sequence Data; Peptides; Protein Denaturation; Recombinant Proteins; Sepharose; Tetrahydrofolate Dehydrogenase | 1999 |
Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme.
Topics: Amino Acid Sequence; Base Sequence; beta-Galactosidase; Chromatography, Agarose; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Escherichia coli; Heating; Histidine; Molecular Sequence Data; Nickel; Pyrococcus; Recombinant Fusion Proteins; Sepharose | 2000 |
Comparison of the effect of histidine-rich glycoprotein and 6-aminohexanoic acid on plasmin production and fibrinolysis in vitro.
Topics: Aminocaproic Acid; Antifibrinolytic Agents; Fibrinolysin; Fibrinolysis; Glycoproteins; Histidine; Humans; Kinetics; Plasminogen; Protein Binding; Protein Structure, Tertiary; Sepharose | 2000 |
Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis.
Topics: Automation; Deoxyribonucleases, Type II Site-Specific; DNA, Bacterial; Enzymes; Escherichia coli; Gene Library; Genes, Bacterial; Histidine; Immunomagnetic Separation; Microspheres; Mutagenesis; Plasmids; Protein Engineering; Recombinant Fusion Proteins; Selection, Genetic; Sepharose; Time Factors | 2000 |
Apo(a)-kringle IV-type 6: expression in Escherichia coli, purification and in vitro refolding.
Topics: Apolipoproteins A; Base Sequence; Cloning, Molecular; Escherichia coli; Histidine; Iodine Radioisotopes; Kringles; Lysine; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Polymerase Chain Reaction; Protein Engineering; Protein Folding; Recombinant Proteins; Sepharose | 2000 |
One-step purification of rabbit histidine rich glycoprotein by dye-ligand affinity chromatography with metal ion requirement.
Topics: Animals; Benzenesulfonates; Blotting, Western; Carbohydrate Metabolism; Chromatography, Affinity; Cobalt; Coloring Agents; Electrophoresis, Polyacrylamide Gel; Glycosylation; Histidine; Hydrogen-Ion Concentration; Imidazoles; Ions; Isoelectric Focusing; Ligands; Nickel; Protein Binding; Proteins; Rabbits; Sepharose; Trityl Compounds; Zinc | 2000 |
Copurification of the Lac repressor with polyhistidine-tagged proteins in immobilized metal affinity chromatography.
Topics: Aspartic Acid; Bacterial Proteins; Biotinylation; Chromatography, Affinity; Cobalt; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; GTP Phosphohydrolases; Histidine; Lac Operon; Lac Repressors; Mass Spectrometry; Models, Molecular; Molecular Weight; Operator Regions, Genetic; Peptide Mapping; Peptides; Phosphoproteins; Protein Structure, Quaternary; Recombinant Fusion Proteins; Repressor Proteins; Sepharose | 2001 |
Chemiluminescent choline biosensor using histidine-modified peroxidase immobilised on metal-chelate substituted beads and choline oxidase immobilised on anion-exchanger beads co-entrapped in a photocrosslinkable polymer.
Topics: Alcohol Oxidoreductases; Biosensing Techniques; Choline; Enzymes, Immobilized; Histidine; Horseradish Peroxidase; Hydrogen Peroxide; Luminescent Measurements; Sepharose | 2000 |
Modification of histidine (B10) is the causative agent for a superactive form of insulin.
Topics: Amino Acid Substitution; Amino Acids; Aspartic Acid; Bicarbonates; Histidine; Insulin; Sepharose; Structure-Activity Relationship | 2002 |
Analysis of surface properties of human cancer cells using derivatized beads.
Topics: Animals; Arginine; Cell Membrane; Chemokine CCL20; Chemokines, CC; Concanavalin A; Histidine; Histocytochemistry; Humans; Macrophage Inflammatory Proteins; Mice; Mice, Nude; Microspheres; Receptors, CCR6; Receptors, Chemokine; Sepharose; Tumor Cells, Cultured | 2002 |
Purification of histidine-tagged T4 RNA ligase from E. coli.
Topics: Bacteriophage T4; Base Sequence; Chromatography, Affinity; Escherichia coli; Histidine; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Recombinant Fusion Proteins; RNA Ligase (ATP); Sepharose; Viral Proteins | 2002 |
Assays and affinity purification of biotinylated and nonbiotinylated forms of double-tagged core RNA polymerase II from Saccharomyces cerevisiae.
Topics: Biochemistry; Biotinylation; Carbon-Nitrogen Ligases; DNA; Escherichia coli; Escherichia coli Proteins; Glutathione Transferase; Histidine; Nickel; Protein Binding; Repressor Proteins; RNA Polymerase II; Saccharomyces cerevisiae; Sepharose; Time Factors; Transcription Factors | 2003 |
The Kaposi's sarcoma-associated herpesvirus complement control protein mimics human molecular mechanisms for inhibition of the complement system.
Topics: Amino Acid Motifs; Animals; CHO Cells; Chromatography; Circular Dichroism; Complement C3b; Complement C4b; Complement System Proteins; Cricetinae; DNA Primers; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Herpesvirus 8, Human; Histidine; Humans; Kinetics; Leucine; Lysine; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Phenylalanine; Protein Structure, Tertiary; Recombinant Proteins; Sepharose; Surface Plasmon Resonance; Viral Proteins | 2004 |
Purification of recombinant human carbonic anhydrase-II by metal affinity chromatography without incorporating histidine tags.
Topics: Binding Sites; Carbonic Anhydrase II; Chromatography; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Histidine; Humans; Imino Acids; Plasmids; Protein Binding; Recombinant Proteins; Sepharose | 2004 |
Multivalent interactions of calcium/calmodulin-dependent protein kinase II with the postsynaptic density proteins NR2B, densin-180, and alpha-actinin-2.
Topics: Actinin; Amino Acid Sequence; Animals; Baculoviridae; Blotting, Western; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinases; Glutathione; Glutathione Transferase; Histidine; Immunoprecipitation; Insecta; Macromolecular Substances; Male; Mice; Molecular Sequence Data; Multiprotein Complexes; Mutation; Neurons; Phosphorylation; Protein Binding; Protein Isoforms; Protein Structure, Tertiary; Rats; Rats, Sprague-Dawley; Receptors, N-Methyl-D-Aspartate; Sepharose; Sialoglycoproteins; Signal Transduction; Swine; Synapses; Threonine; Xenopus | 2005 |
Purification and characterization of spiralin, the main protein of the Spiroplasma citri membrane.
Topics: Alanine; Bacterial Outer Membrane Proteins; Biochemistry; Buffers; Cell Membrane; Detergents; Electrophoresis; Electrophoresis, Agar Gel; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Glycine; Histidine; Immunoelectrophoresis; Intracellular Membranes; Leucine; Methionine; Molecular Weight; Phenylalanine; Sepharose; Spiroplasma citri; Subcellular Fractions; Threonine; Tryptophan; Tyrosine; Valine | 1977 |
Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces.
Topics: Binding Sites; Chromatography, Affinity; Enzyme-Linked Immunosorbent Assay; Green Fluorescent Proteins; Histidine; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Protein Array Analysis; Sensitivity and Specificity; Sepharose; Surface Properties; Time Factors | 2006 |
Immobilized protein ZZ, an affinity tool for immunoglobulin isolation and immunological experimentation.
Topics: Butylene Glycols; Chromatography, Affinity; Cloning, Molecular; Cross-Linking Reagents; Histidine; Immunoglobulin G; Immunoprecipitation; Protein Structure, Tertiary; Recombinant Proteins; Sepharose; Staphylococcal Protein A | 2006 |
Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography.
Topics: Chromatography, Liquid; DNA; Histidine; Plasmids; Sepharose; Temperature | 2007 |
Affinity purification of plasmid DNA directly from crude bacterial cell lysates.
Topics: Aspartic Acid; Bacteria; Bacterial Proteins; Chromatography, Affinity; DNA, Superhelical; Escherichia coli; Escherichia coli Proteins; Freeze Drying; Green Fluorescent Proteins; Histidine; Isopropyl Thiogalactoside; Lac Repressors; Plasmids; Recombinant Fusion Proteins; Repressor Proteins; Reproducibility of Results; Ribonuclease, Pancreatic; Sepharose | 2007 |
[Preparation of metal chelate affinity chromatographic medium and its application in the purification of 6 x histidine-tagged protein].
Topics: Aspartic Acid; Chelating Agents; Chromatography, Affinity; Epoxy Compounds; Histidine; Polymers; Recombinant Fusion Proteins; Sepharose | 2007 |
Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteins.
Topics: Endopeptidases; Enzyme Stability; Enzymes, Immobilized; Glutaral; Histidine; Kinetics; Oligopeptides; Potyvirus; Recombinant Fusion Proteins; Sepharose; Viral Proteins | 2009 |
A heme fusion tag for protein affinity purification and quantification.
Topics: Amino Acid Sequence; Azurin; Binding, Competitive; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Heme; Histidine; Maltose-Binding Proteins; Molecular Sequence Data; Molecular Structure; Protein Binding; Recombinant Fusion Proteins; Resins, Synthetic; Sepharose; Spectrophotometry | 2010 |
A comparison study between polymeric ligand and monomeric ligand for oligopeptide adsorption.
Topics: Adsorption; Aspartic Acid; Calorimetry, Differential Scanning; Histidine; Leucine; Ligands; Models, Molecular; Oligopeptides; Polymers; Sepharose | 2011 |
Analysis of nucleotides binding to chromatography supports provided by nuclear magnetic resonance spectroscopy.
Topics: Chromatography, Agarose; Histidine; Models, Chemical; Nuclear Magnetic Resonance, Biomolecular; Nucleotides; Sepharose | 2011 |
Modulation of the distribution of small proteins within porous matrixes by smart-control of the immobilization rate.
Topics: Escherichia coli; Green Fluorescent Proteins; Histidine; Imidazoles; Immobilized Proteins; Kinetics; Microspheres; Models, Theoretical; Porosity; Sepharose | 2011 |
Binding analysis between L-histidine immobilized and oligonucleotides by SPR and NMR.
Topics: Histidine; Kinetics; Magnetic Resonance Spectroscopy; Oligonucleotides; Sepharose; Surface Plasmon Resonance | 2013 |
An improved strategy for easy process monitoring and advanced purification of recombinant proteins.
Topics: Animals; Bacterial Proteins; Carrier Proteins; DNA-Directed DNA Polymerase; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Genetic Vectors; Heme-Binding Proteins; Hemeproteins; Histidine; Rats; Recombinant Fusion Proteins; Sepharose; Vesicular Transport Proteins | 2013 |
An ectotherm homologue of human predicted gene NAT16 encodes histidine N-acetyltransferase responsible for Nα-acetylhistidine synthesis.
Topics: Acetyl Coenzyme A; Acetylation; Acetyltransferases; Alternative Splicing; Amino Acid Sequence; Animals; Brain; Chromatography, Gel; Cloning, Molecular; Fishes; Histidine; Humans; Molecular Sequence Data; Sepharose; Sequence Homology, Amino Acid | 2014 |
Integrative refolding and purification of histidine-tagged protein by like-charge facilitated refolding and metal-chelate affinity adsorption.
Topics: Adsorption; Chelating Agents; Chromatography, Affinity; Coordination Complexes; Copper; Gels; Green Fluorescent Proteins; Histidine; Imino Acids; Metals; Nickel; Oligopeptides; Protein Refolding; Proteins; Recombinant Proteins; Resins, Synthetic; Sepharose | 2014 |
Selective adsorption of antiphospholipid and anti-dsDNA autoantibodies on histidine based pseudobioaffinity adsorbent from sera of patients with systemic lupus erythematosus (SLE).
Topics: Adsorption; Adult; Aged; Autoantibodies; Chromatography, Liquid; DNA; Epoxy Compounds; Female; Histidine; Humans; Immunoglobulin G; Immunoglobulin M; Lupus Erythematosus, Systemic; Male; Middle Aged; Sepharose; Young Adult | 2015 |
Rapid large-scale purification of myofilament proteins using a cleavable His6-tag.
Topics: Automation; Cardiac Myosins; Chromatography, Affinity; Dextrans; Endopeptidases; Escherichia coli; Histidine; Humans; Myofibrils; Myosin Light Chains; Nickel; Oligopeptides; Recombinant Proteins; Sepharose; Troponin C; Troponin I; Troponin T | 2015 |
Grafting iminodiacetic acid on silica nanoparticles for facilitated refolding of like-charged protein and its metal-chelate affinity purification.
Topics: Chelating Agents; Chemistry Techniques, Analytical; Chromatography, Affinity; Green Fluorescent Proteins; Histidine; Imino Acids; Indicators and Reagents; Metals; Methacrylates; Nanoparticles; Oligopeptides; Protein Folding; Sepharose; Silicon Dioxide | 2016 |
A facile method to oriented immobilization of His-tagged BirA on Co
Topics: Biotinylation; Carbon-Nitrogen Ligases; Catalysis; Cobalt; Coordination Complexes; Enzymes, Immobilized; Escherichia coli; Escherichia coli Proteins; Histidine; Hydrogen Peroxide; Microspheres; Nitrilotriacetic Acid; Repressor Proteins; Sepharose | 2019 |
Selective Immobilization of Fluorescent Proteins for the Fabrication of Photoactive Materials.
Topics: Alginates; Biosensing Techniques; Gene Expression; Graphite; Green Fluorescent Proteins; Histidine; Hydrogels; Hydrogen-Ion Concentration; Immobilized Proteins; Light; Luminescent Proteins; Methacrylates; Nanoparticles; Oligopeptides; Photochemical Processes; Recombinant Fusion Proteins; Red Fluorescent Protein; Sepharose | 2019 |
Development of histidine-tagged cyclic peptide functionalized monolithic material for the affinity purification of antibodies in biological matrices.
Topics: Adsorption; Antibodies, Monoclonal; Chelating Agents; Chromatography, Affinity; Histidine; Humans; Immunoglobulin G; Ligands; Peptides; Peptides, Cyclic; Sepharose | 2021 |
Roles of Fe-Histidine bonds in stability of hemoglobin: Recognition of protein flexibility by Q Sepharose.
Topics: Heme; Hemoglobins; Histidine; Humans; Sepharose; Spectrum Analysis, Raman | 2021 |
Fabrication of copolymer brushes grafted superporous agarose gels: Towards the ultimate ideal particles for efficient affinity chromatography.
Topics: Chromatography, Affinity; Gels; Histidine; Polymers; Proteins; Sepharose | 2022 |
Preparation of nickel-chelated iminodiacetate-functionalized macroporous agarose monolith using modular and clickable building blocks for affinity separation of histidine-tagged recombinant proteins.
Topics: Alkynes; Azides; Chromatography, Affinity; Histidine; Imidazoles; Indicators and Reagents; Ions; Nickel; Polymers; Recombinant Proteins; Sepharose | 2022 |
Synthesis of micron-sized magnetic agarose beads chelated with nickel ions towards the affinity-based separation of histidine-tagged/rich proteins.
Topics: Amines; Emulsions; Histidine; Ions; Magnetic Phenomena; Nickel; Sepharose; Serum Albumin, Bovine | 2023 |