histidine has been researched along with nitrilotriacetic acid in 183 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 2 (1.09) | 18.7374 |
| 1990's | 24 (13.11) | 18.2507 |
| 2000's | 77 (42.08) | 29.6817 |
| 2010's | 69 (37.70) | 24.3611 |
| 2020's | 11 (6.01) | 2.80 |
| Authors | Studies |
|---|---|
| Chevion, M; Katzhendler, J; Kohen, R; Korbashi, P | 1 |
| Döbeli, H; Hochuli, E; Schacher, A | 1 |
| Botting, CH; Randall, RE | 1 |
| Jørgensen, M; Koch, T; Larsson, C; Nielsen, PE; Orum, H; Stanley, C | 1 |
| Dietrich, C; Schmitt, L; Tampé, R | 1 |
| Gershon, PD; Khilko, S | 1 |
| Gennis, RB; Mitchell, DM | 1 |
| Crowe, J; Döbeli, H; Gentz, R; Henco, K; Hochuli, E; Stüber, D | 1 |
| Island, MD; Maneval, D; Mobley, HL; Sriwanthana, B | 1 |
| Funk, CD; Marnett, LJ; Reddy, RG; Yamamoto, S; Yoshimoto, T | 1 |
| Hess, H; Schmitt, J; Stunnenberg, HG | 1 |
| Boscheinen, O; Dietrich, C; Scharf, KD; Schmitt, L; Tampé, R | 1 |
| Burgess, RR; McMahan, SA | 1 |
| Crowe, J; Masone, BS; Ribbe, J | 2 |
| Dougherty, JP; Dunn, KE; Gibbs, CS; Paborsky, LR | 1 |
| Ji, Z; Miller, LJ; Pinon, DI | 1 |
| Brown, KG; Grant, KM; McCready, BP; Mottram, JC | 1 |
| Hidaka, Y; Inouye, M; Park, H | 1 |
| Deutscher, SL; Komissarov, AA; Marchbank, MT | 1 |
| Dienes, Z; Keller, TA; Schmid, EL; Vogel, H | 1 |
| Edebratt, F; Hämäläinen, M; Hansson, A; Karlsson, AF; Lidholm, J; Magnusson, K; Nieba, L; Nieba-Axmann, SE; Persson, A; Plückthun, A | 1 |
| Arimura, Y; Fujii, A; Hashimoto, Y; Ishida-Okawara, A; Ito, M; Mine, E; Mizuno, S; Nagasawa, T; Ohashi, YY; Suzuki, K; Tanokura, M; Tomizawa, K; Yamagoe, S; Yamamoto, T | 1 |
| deCastro, MJ; Ho, CH; Stewart, RJ | 1 |
| Freimuth, P; Hainfeld, JF; Halsey, CM; Liu, W; Powell, RD | 1 |
| Beresten, SF; Ellis, NA; Naureckiene, S; Stan, R; van Brabant, AJ; Ye, T | 1 |
| Altin, JG; Parish, CR; van Broekhoven, CL; Vassiliou, G | 1 |
| Chasteen, ND; Dixon, BK; He, QY; MacGillivray, RT; Mason, AB; Nguyen, V; Pakdaman, R; Tam, BM; Woodworth, RC | 1 |
| Bennett, TA; Lynam, E; Neldon, D; Prossnitz, E; Sklar, LA; Vilven, J | 1 |
| Gaub, HE; Ludwig, M; Schmitt, L; Tampé, R | 1 |
| Callis, J; Colón, E; Dahmus, ME; Ling, R | 1 |
| Baumann, CG; Ferguson, AL; Hoggett, JG; Hughes, AD; Scott, DJ; Tufail, U | 1 |
| Bornhorst, JA; Falke, JJ | 1 |
| Ichihashi, K; Osawa, T; Toyokuni, S; Uchida, K | 1 |
| Curiel, DT; Pereboev, A; Pereboeva, L | 1 |
| Ebright, RH; Ebright, YW; Kapanidis, AN | 1 |
| Lauer, SA; Nolan, JP | 1 |
| Unrau, PJ; Wang, QS | 1 |
| Ngamkitidechakul, C; Twining, SS | 1 |
| Chasteen, ND; He, QY; Woodworth, RC | 1 |
| Alloza, I; Hawthorne, S; Martens, E; Vandenbroeck, K | 1 |
| Gu, H; Guo, Z; Xu, B; Xu, C; Xu, K; Zhang, X; Zheng, R; Zhong, X | 1 |
| Guignet, EG; Hovius, R; Vogel, H | 1 |
| Javitch, JA | 1 |
| Gizeli, E; Glad, J | 1 |
| Gu, H; Guo, Z; Liu, H; Xu, B; Xu, C; Xu, K; Zhang, X; Zheng, R | 1 |
| Ataka, K; Giess, F; Haber-Pohlmeier, S; Heberle, J; Knoll, W; Naumann, R; Richter, B | 1 |
| Allen, TM; Das, D; Suresh, MR | 1 |
| Johnson, DL; Martin, LL | 1 |
| Bernaudat, F; Büllow, L | 1 |
| Blankespoor, R; Limoges, B; Schöllhorn, B; Syssa-Magalé, JL; Yazidi, D | 1 |
| Cosnier, S; Gondran, C; Haddour, N | 1 |
| Gandolfi, AJ; Kirkpatrick, DS; Liebler, DC; Tsaprailis, G; Weldon, SF | 1 |
| Lata, S; Piehler, J | 1 |
| Akatsuka, S; Lee, WH; Miyagawa-Hayashino, A; Ozeki, M; Shirase, T; Toyokuni, S; Uchida, K | 1 |
| Brock, R; Lata, S; Piehler, J; Reichel, A; Tampé, R | 1 |
| Dunsmore, C; Malone, K; Patterson, A; Turner, NJ; Walkinshaw, MD; Wear, MA | 1 |
| Ahmed, T; Dorwin, S; Harlan, J; Holzman, T; Johnson, R; Severin, J; Shen, J; Smith, R; Vogt, A; Wang, J | 1 |
| Altin, JG; van Broekhoven, CL | 1 |
| Athavankar, S; Boonyarattanakalin, S; Peterson, BR; Sun, Q | 1 |
| Goldsmith, CR; Jaworski, J; Lippard, SJ; Sheng, M | 1 |
| Bauersachs, S; Blum, H; Donzeau, M; Nagel, W; Reichelt, P; Röhnisch, T | 1 |
| Gavutis, M; Lata, S; Piehler, J; Tampé, R | 1 |
| Coorssen, JR; Howell, JM; Turner, RJ; Winstone, TL | 1 |
| Chung, BH; Lee, K; Lee, KY; Lim, YT | 1 |
| He, M; Khan, F; Taussig, MJ | 1 |
| Mulder, A; Schulze, K; Tampé, R; Tinazli, A | 1 |
| Klenkar, G; Liedberg, B; Piehler, J; Tampé, R; Tinazli, A; Valiokas, R | 1 |
| Bumba, L; Komenda, J; Nebesarova, J; Promnares, K; Tichy, M; Vacha, F | 1 |
| Cheng, Y; Li, Q; Liao, Y | 1 |
| Asanuma, M; Hirota, H; Saikawa, W; Saito, K; Yokoyama, S; Yoshitani, N | 1 |
| Gierasch, LM; Krishnan, B; Szymanska, A | 1 |
| Franses, EI; Holland, DP; Kang, E; Kim, JM; Lee, GU; McClellan, SJ; Park, JW; Park, K; Thompson, DH | 1 |
| Escalante, M; Huskens, J; Maury, P; Péter, M; Reinhoudt, DN; Subramaniam, V | 1 |
| Walkinshaw, MD; Wear, MA | 1 |
| Chen, CT; Chen, WY; Chen, YC; Li, YC; Lin, YS; Tsai, PJ | 1 |
| Baker, GL; Bruening, ML; Dai, J; Jain, P; Sun, L | 1 |
| Jeyakumar, M; Katzenellenbogen, JA; Kim, SH | 1 |
| Al Furoukh, N; Cohen, M; Piehler, J; Reichel, A; Schaible, D; Schreiber, G | 1 |
| Briñas, RP; Hainfeld, JF; Hu, M; Lymar, ES; Qian, L | 1 |
| Huskens, J; Ludden, MJ; Mulder, A; Schulze, K; Subramaniam, V; Tampé, R | 1 |
| Ayres, L; Bergia, A; Nolte, RJ; Rowan, AE; Samorì, B; Valle, F; Zuccheri, G | 1 |
| Petty, KJ | 2 |
| Dukovski, D; Kelly, DF; Walz, T | 1 |
| Grailhe, R; Kim, J; Kwon, DY; Park, HY; Ryu, J; Song, R | 1 |
| Ackland, ML; Alvarez, A; Grana, A; Hamer, P; Ho, YH; Jamil, R; Kim, S; Linder, MC; Mercer, JF; Michalczyk, A; Moriya, M; Nguyen, L; Ramos, D | 1 |
| Goto, M; Hosogi, T; Kamiya, N; Maruyama, T; Shimada, J; Tominaga, J | 1 |
| Campopiano, DJ; Caniard, A; Gupta, M; Mareque-Rivas, JC; Touceda-Varela, A | 1 |
| Baksh, MM; Brown, S; Finn, MG; Udit, AK | 1 |
| De, M; Rana, S; Rotello, VM | 1 |
| Broyer, RM; Chen, Y; Christman, KL; Li, RC; Maynard, HD; Schopf, E | 1 |
| Clarke, S; Dahan, M; Gouzer, GG; Marchi-Artzner, V; Piehler, J; Pinaud, F; Roullier, V; Schaible, D; You, C | 1 |
| Neff, D; Norton, ML; Shen, W; Zhong, H | 1 |
| Balland, V; Lecomte, S; Limoges, B | 1 |
| Erben, CM; Goodman, RP; Ho, WM; Kapanidis, AN; Malo, J; McKee, ML; Turberfield, AJ | 1 |
| André, T; Brock, R; Piehler, J; Reichel, A; Tampé, R; Wiesmüller, KH | 1 |
| Cao, L; Huang, Z; Hwang, P; Szoka, FC; Watson, DS | 1 |
| Gilbreth, RN; Koide, A; Koide, S; Piehler, J; Reichel, A; Wojcik, J | 1 |
| Fujishima, SH; Hamachi, I; Nonaka, H; Ojida, A; Tsukiji, S; Uchinomiya, SH | 1 |
| Baati, R; Barlaam, B; Brellier, M; Mioskowski, C | 1 |
| Bujoli, B; Cinier, M; Fabre, RM; Pecorari, F; Petit, M; Talham, DR; Tellier, C; Williams, MN | 1 |
| Nakayama, T; Namura, M; Noji, H; Tabata, KV; Yokokawa, R | 1 |
| Choi, Y; Grailhe, R; Hong, S; Kim, H; Kim, K; Kwon, D; Park, HY; Ryu, J; Song, R | 1 |
| Bowman, WS; Fried, MG; Hellman, LM; Whiteheart, SW; Zhan, X; Zhao, C | 1 |
| Baker, GL; Bruening, ML; Geiger, JH; Jain, P; Vyas, MK | 1 |
| Blaas, D; Grunwald, C; Piehler, J; Reichel, A; Schulze, K; Tampé, R; Weiss, VU; Wiesmüller, KH | 1 |
| Ijiro, K; Kinjo, M; Matsuo, Y; Mikuni, S; Nagakawa, K; Niikura, K; Ohtake, N; Sawa, H; Suzuki, T | 1 |
| Gautrot, JE; Huck, WT; Ramstedt, M; Welch, M | 1 |
| Cao, L; Huang, Z; Platt, V; Riviere, K; Szoka, FC; Tiffany, M | 1 |
| Bhagawati, M; Lata, S; Piehler, J; Tampé, R | 1 |
| Mack, NH; Nuzzo, RG; Thompson, LB | 1 |
| Iversen, L; Liu, YC; Martinez, KL; Nygård, J; Park, J; Rieben, N; Sørensen, BS | 1 |
| Kimura, S; Makino, A; Nakamura, I; Ohmae, M | 1 |
| Cui, XS; Dowdy, SF; Eguchi, A; Gogoi, K; June, RK | 1 |
| Chevalier, S; Cuestas-Ayllon, C; de la Fuente, JM; Feracci, H; Grazu, V; Luna, M | 1 |
| Hanson, PI; Merrill, SA | 1 |
| Jiang, S; Pun, SH; Wang, CH | 1 |
| Ackerson, CJ; Hainfeld, JF; Powell, RD | 1 |
| Choi, Y; Hong, S; Kim, H; Kim, J; Kwon, H; Song, R | 1 |
| Arai, S; Murata, A; Ozaki, M; Takabayashi, M; Takeoka, S; Yoon, SI | 1 |
| Anderson, T; DeRocco, V; Erie, DA; Piehler, J; Weninger, K | 1 |
| Cao, L; Platt, VM; Szoka, FC; Venditto, VJ; Watson, DS | 1 |
| Chen, Y; Cheng, W; Ding, L; Ding, S; Ju, H; Yan, F; Yin, Y | 1 |
| Bouazaoui, M; Boukherroub, R; Burkhard, P; Chazalviel, JN; Gouget-Laemmel, AC; Maalouli, N; Ozanam, F; Pinchemel, B; Szunerits, S; Yang, Y | 1 |
| Chaiken, I; Ishino, T; Mero, A; Pasut, G; Veronese, FM | 1 |
| Bamdad, C; Calhoun, AK; Hatziioannou, VM; Smagghe, BJ; Stevens, MD; Thompson, AB; Wotkowicz, MT | 1 |
| Irusta, S; Kohane, DS; Langer, R; McKenna, M; Mizrahi, B; Myint, M; Stefanescu, C; Yedidsion, L | 1 |
| Gonchar, MV; Horak, D; Potocka, N; Rzhepetskyy, YA; Stasyk, OV; Zakalska, OM; Zakalskiy, AE | 1 |
| Baumgaertner, P; Guillaume, P; Irving, M; Luescher, IF; Schmidt, J; Speiser, D | 1 |
| Gulka, CP; Haselton, FR; Swartz, JD; Wright, DW | 1 |
| Chen, CT; Liang, MR; Lin, YC | 1 |
| Guo, M; Liu, X; Nie, Z; Qing, M; Xu, X; Yao, S; Zhou, J | 1 |
| Gerwert, K; Güldenhaupt, J; Kötting, C; Pinkerneil, P | 1 |
| Campbell, WH; Henig, J; Plumeré, N | 1 |
| Chen, G; Cui, F; Lan, W; Liu, R; Tan, F; Yushupujiang, M | 1 |
| Fang, TY; Su, LY; Tseng, WC | 1 |
| Spatz, JP; Wegner, SV | 1 |
| Bartlett, PN; Boer, H; Sosna, M | 1 |
| Biswas, A; Chakraborty, I; Ghosh, S; Jana, B; Kurkute, P; Mondal, G; Saha, A | 1 |
| Hayat, A; Marty, JL; Sassolas, A | 1 |
| Guo, M; Huang, Y; Lei, C; Nie, Z; Wang, M; Yao, S | 1 |
| Kirk, WR | 1 |
| Beutel, O; Birkholz, O; Herrmann, A; Nikolaus, J; Piehler, J; Schmidt, T; You, C | 1 |
| Oberoi-Khanuja, TK; Rajalingam, K | 1 |
| Anthony, KC; Piehler, J; Pomeranz Krummel, DA; You, C | 1 |
| Alsteens, D; Hilbert, M; Müller, DJ; Pfreundschuh, M; Steinmetz, MO | 1 |
| Piehler, J; You, C | 1 |
| Lai, RY; Zaitouna, AJ | 1 |
| Cabanas-Danés, J; Huskens, J; Jonkheijm, P; Karperien, M; Landman, E; Rodrigues, ED; van Blitterswijk, C; van Weerd, J; Verrips, T | 1 |
| Cardoso, MC; Kollmannsperger, A; Labòria, N; Natale, F; Rajan, M; Tampé, R; Wieneke, R | 1 |
| Hellmert, M; Korth, C; Müller-Schiffmann, A; Peters, MS; Schrader, T | 1 |
| Ahn, DR; Park, YJ; Thai, HB; Yu, JK | 1 |
| Dogterom, M; Lamers, D; Lof, A; Roth, S; Taberner, N; Zeijlemaker, H | 1 |
| Blazevic, V; Hytönen, VP; Ihalainen, TO; Koho, T; Kulomaa, MS; Marjomäki, V; Rahikainen, R; Stark, M; Tampé, R; Uusi-Kerttula, H; Wieneke, R | 1 |
| Cherkouk, C; Haase-Kohn, C; Keller, A; Laube, M; Lenk, J; Neuber, C; Ou, X; Pietzsch, J; Rebohle, L; Skorupa, W | 1 |
| Dmitriev, RI; O'Donnell, N; Papkovsky, DB | 1 |
| Bajaj, R; Benjamin, CJ; Guo, F; Hyun, SH; Jiang, W; Krynski, K; Stauffacher, CV; Thompson, DH; Wright, KJ; Yu, G | 1 |
| Schenk, FC; Spatz, JP; Wegner, SV | 1 |
| Bryant, DA; Cartron, M; Hunter, CN; Leggett, GJ; Morby, J; Xia, S | 1 |
| Kamiya, N; Kimizuka, N; Matsuura, K; Minamihata, K; Nakamura, T; Noguchi, T; Watanabe, K | 1 |
| Demizu, Y; Hattori, T; Kurihara, M; Misawa, T; Naito, M; Okitsu, K; Shoda, T | 1 |
| Beloqui, A; Delaittre, G; Ferrer, M; Keller, D; Martínez-Martínez, M | 1 |
| Demizu, Y; Hattori, T; Kurihara, M; Misawa, T; Naito, M; Ohoka, N; Okitsu, K; Shibata, N; Yamazaki, N | 1 |
| Blum, C; Cabanas-Danés, J; Cazade, PA; Huskens, J; Jonkheijm, P; O'Mahony, S; Prangsma, J; Subramaniam, V; Thompson, D; Tromp, E; Wasserberg, D | 1 |
| Cabanas-Danés, J; Huskens, J; Jonkheijm, P; Subramaniam, V; Wasserberg, D | 1 |
| Gatterdam, K; Gatterdam, V; Joest, EF; Tampé, R | 1 |
| An, SSA; Hwang, A; Jose, L; Lee, C; Paik, HJ; Park, JH; Shim, K; Song, JK | 1 |
| Cao, H; Han, L; Huang, C; Jia, L; Wang, R; Xu, L; Xu, T | 1 |
| Liu, L; Liu, Q; Tan, X; Wang, X; Zhou, F | 1 |
| Li, AC; Li, Y; Xu, Q | 1 |
| Chang, YY; Jiang, N; Lai, YT; Li, H; Sun, H; Yang, X; Yang, Y | 1 |
| Fu, T; Han, Y; Hao, P; Hu, S; Kang, J; Liu, G; Pan, L; Zhang, X; Zheng, M | 1 |
| Brüchert, S; Gatterdam, K; Joest, EF; Tampé, R | 1 |
| Bogetti, X; Rule, GS; Saxena, S; Singewald, K; Sinha, K | 1 |
| Hamachi, I; Heroven, C; Hoon Oh, J; Radu Aricescu, A; Tamura, T; Thimaradka, V; Yuzaki, M | 1 |
| Ackermann, K; Bode, BE; Norman, DG; Wort, JL | 1 |
| Ackermann, K; Arya, S; Bode, BE; Stewart, AJ; Wort, JL | 1 |
| Han, C; Jiang, M; Kang, Q; Liu, L; Wang, P; Wang, X; Zhang, T; Zhou, F | 1 |
| Gao, X; Han, L; Ji, F; Jia, L; Meng, X; Ren, J; Xiang, X | 1 |
| Dixit, CK | 1 |
| Chung, YH; Steinmetz, NF; Volckaert, BA | 1 |
| Hao, Y; Liu, P; Lv, Y; Ma, X; Xia, N | 1 |
2 review(s) available for histidine and nitrilotriacetic acid
| Article | Year |
|---|---|
6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification.
Topics: Chelating Agents; Chromatography, Affinity; Escherichia coli; Genetic Vectors; Histidine; Nickel; Nitrilotriacetic Acid; Operator Regions, Genetic; Promoter Regions, Genetic; Recombinant Fusion Proteins | 1994 |
Multivalent chelators for spatially and temporally controlled protein functionalization.
Topics: Animals; Biosensing Techniques; Chelating Agents; Histidine; Humans; Ions; Lipids; Nanotechnology; Nitrilotriacetic Acid; Oxygen; Protein Interaction Mapping; Proteins; Recombinant Proteins; Spectrophotometry; Surface Properties | 2014 |
181 other study(ies) available for histidine and nitrilotriacetic acid
| Article | Year |
|---|---|
Iron mediates paraquat toxicity in Escherichia coli.
Topics: Catalase; Deferoxamine; Drug Synergism; Enzyme Induction; Escherichia coli; Histidine; Hydroxides; Hydroxyl Radical; Iron; Mannitol; Mathematics; Nitrilotriacetic Acid; Paraquat; Superoxide Dismutase | 1986 |
New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.
Topics: Adsorption; Animals; Chelating Agents; Histidine; Ligands; Metals; Nickel; Nitrilotriacetic Acid; Peptides; Proteins; Spectrophotometry, Atomic | 1987 |
Reporter enzyme-nitrilotriacetic acid-nickel conjugates: reagents for detecting histidine-tagged proteins.
Topics: Affinity Labels; Alkaline Phosphatase; Antibodies, Monoclonal; Chromatography, Affinity; Escherichia coli; Histidine; Hydrogen-Ion Concentration; Indicators and Reagents; Nickel; Nitrilotriacetic Acid; Recombinant Proteins | 1995 |
Sequence-specific purification of nucleic acids by PNA-controlled hybrid selection.
Topics: Base Composition; Base Sequence; Binding Sites; Chromatography, Affinity; Histidine; Hot Temperature; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Nucleic Acid Hybridization; Nucleic Acids; Oligonucleotide Probes; Oligonucleotides; Peptides; Recombinant Fusion Proteins; RNA | 1995 |
Molecular organization of histidine-tagged biomolecules at self-assembled lipid interfaces using a novel class of chelator lipids.
Topics: Amines; Amino Acid Sequence; Chelating Agents; Dimyristoylphosphatidylcholine; Histidine; Indicators and Reagents; Models, Structural; Molecular Conformation; Molecular Sequence Data; Nitrilotriacetic Acid; Peptides; Phosphatidylethanolamines; Recombinant Fusion Proteins; Sequence Tagged Sites | 1995 |
Stable chelating linkage for reversible immobilization of oligohistidine tagged proteins in the BIAcore surface plasmon resonance detector.
Topics: Biosensing Techniques; Chelating Agents; Histidine; Membrane Proteins; Nitrilotriacetic Acid; Protein Binding; Spectrum Analysis; Vaccinia virus; Viral Proteins | 1995 |
Rapid purification of wildtype and mutant cytochrome c oxidase from Rhodobacter sphaeroides by Ni(2+)-NTA affinity chromatography.
Topics: Amino Acid Sequence; Base Sequence; Chromatography, Affinity; Electron Transport Complex IV; Histidine; Molecular Sequence Data; Mutation; Nitrilotriacetic Acid; Oligodeoxyribonucleotides; Organometallic Compounds; Rhodobacter sphaeroides | 1995 |
Single-step purification of Proteus mirabilis urease accessory protein UreE, a protein with a naturally occurring histidine tail, by nickel chelate affinity chromatography.
Topics: Amino Acid Sequence; Bacterial Proteins; Carrier Proteins; Chelating Agents; Chromatography, Affinity; Escherichia coli; Genetic Complementation Test; Histidine; Molecular Sequence Data; Molecular Weight; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Proteus mirabilis; Recombinant Proteins; Sequence Analysis; Urease | 1994 |
Expression of porcine leukocyte 12-lipoxygenase in a baculovirus/insect cell system and its characterization.
Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Animals; Arachidonate 12-Lipoxygenase; Arachidonic Acid; Baculoviridae; Cells, Cultured; Chromatography, Affinity; Cloning, Molecular; Histidine; Hydroxyeicosatetraenoic Acids; Kinetics; Leukocytes; Moths; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Precipitin Tests; Protein Engineering; Recombinant Fusion Proteins; Substrate Specificity; Swine | 1994 |
Affinity purification of histidine-tagged proteins.
Topics: Animals; Binding Sites; Chromatography, Affinity; Evaluation Studies as Topic; HeLa Cells; Histidine; Humans; Nickel; Nitrilotriacetic Acid; Protein Denaturation; Recombinant Proteins | 1993 |
Functional immobilization of a DNA-binding protein at a membrane interface via histidine tag and synthetic chelator lipids.
Topics: Adsorption; Amines; Base Sequence; Biophysical Phenomena; Biophysics; Chelating Agents; Heat-Shock Proteins; Histidine; Lipid Metabolism; Membranes, Artificial; Microscopy, Fluorescence; Molecular Sequence Data; Nitrilotriacetic Acid; Peptides; Plant Proteins; Protein Binding; Recombinant Fusion Proteins; Trans-Activators | 1996 |
Single-step synthesis and characterization of biotinylated nitrilotriacetic acid, a unique reagent for the detection of histidine-tagged proteins immobilized on nitrocellulose.
Topics: Biotin; Blotting, Western; Collodion; HeLa Cells; Histidine; Humans; Lysine; Nickel; Nitrilotriacetic Acid; Recombinant Proteins; Saccharomyces cerevisiae; Sigma Factor | 1996 |
One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.
Topics: Amino Acid Sequence; Bacterial Proteins; Chelating Agents; Chromatography, Affinity; Escherichia coli; Gene Expression; Genetic Vectors; Histidine; Molecular Sequence Data; Nitrilotriacetic Acid; Organometallic Compounds; Plasmids; Protein Denaturation; Recombinant Proteins; Recombination, Genetic | 1995 |
One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.
Topics: Bacterial Proteins; Chromatography, Affinity; Escherichia coli; Genetic Testing; Genetic Vectors; Histidine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Plasmids; Recombinant Proteins; Resins, Plant; Solubility | 1996 |
A nickel chelate microtiter plate assay for six histidine-containing proteins.
Topics: Antibodies, Monoclonal; Chelating Agents; Enzyme-Linked Immunosorbent Assay; Histidine; Indicators and Reagents; Interleukin-8; Ligands; Lysine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Proteins; Recombinant Proteins; Reproducibility of Results; Sensitivity and Specificity; Sequence Tagged Sites; Transferrin | 1996 |
Development of magnetic beads for rapid and efficient metal-chelate affinity purifications.
Topics: Chromatography, Affinity; Histidine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Polystyrenes; Resins, Synthetic; Tosyl Compounds | 1996 |
Gene disruptions indicate an essential function for the LmmCRK1 cdc2-related kinase of Leishmania mexicana.
Topics: Alleles; Animals; Blotting, Northern; Blotting, Southern; Blotting, Western; Caseins; Chromosome Mapping; DNA, Protozoan; Gene Expression Regulation; Genetic Complementation Test; Histidine; Leishmania mexicana; Molecular Sequence Data; Mutagenesis, Insertional; Nitrilotriacetic Acid; Organometallic Compounds; Plasmids; Ploidies; Protein Kinases; Protozoan Proteins; RNA, Protozoan; Sequence Deletion; Transfection; Trypanosoma brucei brucei | 1996 |
Demonstration of dimer formation of the cytoplasmic domain of a transmembrane osmosensor protein, EnvZ, of Escherichia coli using Ni-histidine tag affinity chromatography.
Topics: Bacterial Outer Membrane Proteins; Chromatography, Affinity; Cytoplasm; Escherichia coli; Escherichia coli Proteins; Gene Expression; Histidine; Multienzyme Complexes; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Recombinant Fusion Proteins; Resins, Plant | 1997 |
The use of Ni-nitrilotriacetic acid agarose for estimation of affinities of hexahistidine-tagged Fab to single-stranded DNA.
Topics: Affinity Labels; Animals; Chelating Agents; DNA, Single-Stranded; Histidine; Immunoglobulin Fab Fragments; Immunoglobulin Variable Region; In Vitro Techniques; Kinetics; Mice; Mutation; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Sepharose | 1997 |
Reversible oriented surface immobilization of functional proteins on oxide surfaces.
Topics: Chelating Agents; Green Fluorescent Proteins; Histidine; Kinetics; Luminescent Proteins; Metals; Nitrilotriacetic Acid; Protein Binding; Proteins; Radioligand Assay; Silanes; Spectrometry, Fluorescence; Sulfhydryl Compounds; Surface Properties | 1997 |
BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip.
Topics: Adenosine Triphosphate; Biosensing Techniques; Chaperonin 10; Chaperonin 60; Chelating Agents; Detergents; Edetic Acid; Histidine; Hydrogen-Ion Concentration; Kinetics; Ligands; Macromolecular Substances; Nickel; Nitrilotriacetic Acid; Polysorbates; Protein Binding; Protein Folding; Sodium Chloride; Surface Properties | 1997 |
A panel set for epitope analysis of myeloperoxidase (MPO)-specific antineutrophil cytoplasmic antibody MPO-ANCA using recombinant hexamer histidine-tagged MPO deletion mutants.
Topics: Animals; Antibodies; Antibodies, Antineutrophil Cytoplasmic; Antibody Specificity; Chromatography; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Epitopes; Escherichia coli; Histidine; Humans; Kidney Diseases; Mutation; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Fragments; Peroxidase; Rabbits; Recombinant Proteins | 1998 |
Motility of dimeric ncd on a metal-chelating surfactant: evidence that ncd is not processive.
Topics: Adenosine Triphosphate; Chelating Agents; Dimerization; Drosophila Proteins; Enzymes, Immobilized; Histidine; Kinesins; Kinetics; Microtubules; Motion; Nitrilotriacetic Acid; Protein Processing, Post-Translational; Recombinant Proteins; Surface-Active Agents | 1999 |
Ni-NTA-gold clusters target His-tagged proteins.
Topics: Adenoviridae; Blotting, Western; Chromatography, Affinity; Forecasting; Gold; Histidine; Microscopy, Electron, Scanning Transmission; Molecular Probes; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Receptors, Virus; Recombinant Proteins; Viral Structural Proteins | 1999 |
Purification of overexpressed hexahistidine-tagged BLM N431 as oligomeric complexes.
Topics: Adenosine Triphosphatases; Amino Acid Motifs; Animals; Bloom Syndrome; Chelating Agents; Chromatography, Affinity; Chromatography, Gel; DNA Helicases; Edetic Acid; Escherichia coli; Gene Expression; Histidine; Humans; Molecular Probes; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; RecQ Helicases; Solubility | 1999 |
Engrafting costimulator molecules onto tumor cell surfaces with chelator lipids: a potentially convenient approach in cancer vaccine development.
Topics: Amines; Animals; B7-1 Antigen; Cancer Vaccines; CD40 Antigens; Cell Division; Cell Membrane; Chelating Agents; Cytotoxicity, Immunologic; Female; Histidine; Lymphocyte Activation; Male; Mast-Cell Sarcoma; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Microscopy, Confocal; Nitrilotriacetic Acid; Protein Binding; T-Lymphocytes; Tumor Cells, Cultured | 2000 |
Mutations at the histidine 249 ligand profoundly alter the spectral and iron-binding properties of human serum transferrin N-lobe.
Topics: Alanine; Animals; Antiporters; Cell Line; Cricetinae; Electron Spin Resonance Spectroscopy; Histidine; Humans; Iron; Kinetics; Ligands; Mutagenesis, Site-Directed; Nitrilotriacetic Acid; Peptide Fragments; Protein Binding; Spectrophotometry, Ultraviolet; Transferrin | 2000 |
Solubilization and display of G protein-coupled receptors on beads for real-time fluorescence and flow cytometric analysis.
Topics: Chemotactic Factors; Computer Systems; Flow Cytometry; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Gene Expression; GTP-Binding Proteins; Histidine; Humans; Microspheres; N-Formylmethionine Leucyl-Phenylalanine; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Receptors, Cell Surface; Receptors, Formyl Peptide; Receptors, Immunologic; Receptors, Peptide; Signal Transduction; Silicon Dioxide; Solubility; Spectrometry, Fluorescence; Transfection; U937 Cells | 2000 |
A metal-chelating microscopy tip as a new toolbox for single-molecule experiments by atomic force microscopy.
Topics: Chelating Agents; Edetic Acid; Histidine; Metals; Microscopy, Atomic Force; Nitrilotriacetic Acid; Peptides; Recombinant Fusion Proteins | 2000 |
Histidine-tagged ubiquitin substitutes for wild-type ubiquitin in Saccharomyces cerevisiae and facilitates isolation and identification of in vivo substrates of the ubiquitin pathway.
Topics: Arabidopsis; Canavanine; Chromatography, Ion Exchange; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Histidine; Immunoblotting; Mutagenesis, Site-Directed; Nitrilotriacetic Acid; Nitrogen; Organometallic Compounds; Plants, Genetically Modified; Plasmids; Protein Binding; RNA Polymerase II; Saccharomyces cerevisiae; Transfection; Ubiquitins; Yeasts | 2000 |
Interaction of sigma 70 with Escherichia coli RNA polymerase core enzyme studied by surface plasmon resonance.
Topics: Bacterial Proteins; Binding, Competitive; DNA-Directed RNA Polymerases; Enzyme Stability; Escherichia coli; Half-Life; Histidine; Holoenzymes; Kinetics; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Sigma Factor; Surface Plasmon Resonance | 2000 |
Purification of proteins using polyhistidine affinity tags.
Topics: Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Histidine; Indicators and Reagents; Membrane Proteins; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Recombinant Proteins | 2000 |
Endogenous formation of protein adducts with carcinogenic aldehydes: implications for oxidative stress.
Topics: Aldehydes; Animals; Antibodies, Monoclonal; Carcinogens; Ferric Compounds; Histidine; Kidney Neoplasms; Kidney Tubules, Proximal; Lipid Peroxidation; Lysine; Male; Models, Chemical; Nitrilotriacetic Acid; Oxidative Stress; Rats; Rats, Wistar; Serum Albumin, Bovine | 2001 |
Phage display of adenovirus type 5 fiber knob as a tool for specific ligand selection and validation.
Topics: Adenoviruses, Human; Capsid; Capsid Proteins; Cloning, Molecular; Histidine; Humans; Ligands; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Library | 2001 |
Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni(2+):nitrilotriacetic Acid (n)-fluorochrome conjugates.
Topics: Fluorescent Dyes; Histidine; Nickel; Nitrilotriacetic Acid; Proteins | 2001 |
Development and characterization of Ni-NTA-bearing microspheres.
Topics: Binding Sites; Chelating Agents; Flow Cytometry; Histidine; Indicators and Reagents; Microspheres; Nitrilotriacetic Acid; Organometallic Compounds; Surface Properties | 2002 |
Purification of histidine-tagged T4 RNA ligase from E. coli.
Topics: Bacteriophage T4; Base Sequence; Chromatography, Affinity; Escherichia coli; Histidine; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Recombinant Fusion Proteins; RNA Ligase (ATP); Sepharose; Viral Proteins | 2002 |
Buffered non-fermenter system for lab-scale production of secreted recombinant His-tagged proteins in Saccharomyces cerevisiae.
Topics: Buffers; Centrifugation; Chromatography, Affinity; Cloning, Molecular; Culture Media; Genes, Tumor Suppressor; Histidine; Humans; Hydrogen-Ion Concentration; Nickel; Nitrilotriacetic Acid; Oligopeptides; Peptides; Protein Biosynthesis; Proteins; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Serpins; Ultrafiltration | 2002 |
Anion exchange in human serum transferrin N-lobe: a model study with variant His249Ala.
Topics: Anions; Binding Sites; Carbonates; Histidine; Humans; Hydrogen-Ion Concentration; Ion Transport; Iron; Kinetics; Nitrilotriacetic Acid; Recombinant Proteins; Spectrophotometry; Transferrin | 2003 |
Cross-linking approach to affinity capture of protein complexes from chaotrope-solubilized cell lysates.
Topics: Affinity Labels; Calreticulin; Cell Line; Cross-Linking Reagents; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Histidine; Humans; Interleukin-12; Nickel; Nitrilotriacetic Acid; Peptides; Plasmids; Protein Denaturation; Proteins; Succinimides; Sulfhydryl Compounds; Urea | 2004 |
Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins.
Topics: Alloys; Histidine; Iron; Magnetics; Nanotechnology; Nitrilotriacetic Acid; Particle Size; Platinum; Protein Binding; Proteins; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared | 2004 |
Reversible site-selective labeling of membrane proteins in live cells.
Topics: Animals; Biotechnology; Cell Line; Cell Membrane; Dose-Response Relationship, Drug; Fluorescence Resonance Energy Transfer; Genetic Techniques; Green Fluorescent Proteins; Histidine; Humans; Ions; Kinetics; Luminescent Proteins; Mice; Microscopy, Fluorescence; Nitrilotriacetic Acid; Protein Binding; Time Factors | 2004 |
Finding needles in haystacks.
Topics: Binding Sites; Fluorescence Resonance Energy Transfer; Green Fluorescent Proteins; Histidine; Ligands; Luminescent Proteins; Models, Biological; Mutation; Nickel; Nitrilotriacetic Acid; Oligonucleotides; Protein Binding; Protein Structure, Tertiary; Proteins | 2004 |
Single-step formation of a biorecognition layer for assaying histidine-tagged proteins.
Topics: Choline; Histidine; Indicators and Reagents; Lipid Bilayers; Nitrilotriacetic Acid; Recombinant Fusion Proteins; Silicon Dioxide | 2004 |
Dopamine as a robust anchor to immobilize functional molecules on the iron oxide shell of magnetic nanoparticles.
Topics: Dopamine; Ferric Compounds; Green Fluorescent Proteins; Histidine; Imidazoles; Luminescent Proteins; Magnetics; Nanotechnology; Nitrilotriacetic Acid; Particle Size; Proteins; Spectrophotometry, Ultraviolet | 2004 |
Oriented attachment and membrane reconstitution of His-tagged cytochrome c oxidase to a gold electrode: in situ monitoring by surface-enhanced infrared absorption spectroscopy.
Topics: Electrochemistry; Electron Transport Complex IV; Enzymes, Immobilized; Gold; Histidine; Lipid Bilayers; Models, Molecular; Nitrilotriacetic Acid; Recombinant Proteins; Spectroscopy, Fourier Transform Infrared; Surface Properties | 2004 |
Comparative evaluation of two purification methods of anti-CD19-c-myc-His6-Cys scFv.
Topics: Antibodies, Monoclonal; Antigens, CD19; Blotting, Western; Chelating Agents; Chromatography, Affinity; Chromatography, Agarose; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Flow Cytometry; Histidine; Hybridomas; Hydrogen-Ion Concentration; Immunoglobulin Variable Region; Ion Exchange Resins; Mass Spectrometry; Molecular Weight; Nickel; Nitrilotriacetic Acid; Plasmids; Proto-Oncogene Proteins c-myc; Solubility; Temperature; Thiogalactosides | 2005 |
Controlling protein orientation at interfaces using histidine tags: an alternative to Ni/NTA.
Topics: Chelating Agents; Electrochemistry; Electrodes; Gold; Histidine; Models, Molecular; Nickel; Nitrilotriacetic Acid; Plastocyanin; Steroid 17-alpha-Hydroxylase; Surface Plasmon Resonance; Thioredoxins | 2005 |
Rapid evaluation of nickel binding properties of His-tagged lactate dehydrogenases using surface plasmon resonance.
Topics: Affinity Labels; Binding Sites; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Histidine; L-Lactate Dehydrogenase; Models, Molecular; Nickel; Nitrilotriacetic Acid; Protein Conformation; Surface Plasmon Resonance | 2005 |
Dense monolayers of metal-chelating ligands covalently attached to carbon electrodes electrochemically and their useful application in affinity binding of histidine-tagged proteins.
Topics: Binding Sites; Biosensing Techniques; Cations, Divalent; Chelating Agents; Copper; Diazonium Compounds; Electrochemistry; Electrodes; Graphite; Histidine; Imino Acids; Kinetics; Ligands; Metals; Microscopy, Fluorescence; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Proteins; Spectrometry, X-Ray Emission; Surface Properties | 2005 |
Electrogeneration of a poly(pyrrole)-NTA chelator film for a reversible oriented immobilization of histidine-tagged proteins.
Topics: Avidin; Biotin; Chelating Agents; Copper; Electrochemistry; Glucose; Glucose Oxidase; Histidine; Hydrogen Peroxide; Hydrogen-Ion Concentration; Nitrilotriacetic Acid; Oxidation-Reduction; Platinum; Polymers; Pyrroles | 2005 |
Proteomic identification of ubiquitinated proteins from human cells expressing His-tagged ubiquitin.
Topics: Amino Acid Sequence; Blotting, Western; Cell Line; Chromatography, Affinity; Eukaryotic Cells; Green Fluorescent Proteins; Histidine; Humans; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Proliferating Cell Nuclear Antigen; Proteins; Proteomics; Substrate Specificity; Transfection; Transgenes; Ubiquitin | 2005 |
Stable and functional immobilization of histidine-tagged proteins via multivalent chelator headgroups on a molecular poly(ethylene glycol) brush.
Topics: Chelating Agents; Histidine; Nitrilotriacetic Acid; Organometallic Compounds; Polyethylene Glycols; Proteins; Sensitivity and Specificity | 2005 |
Susceptibility of actin to modification by 4-hydroxy-2-nonenal.
Topics: Actins; Albumins; Aldehydes; Animals; Antibodies, Monoclonal; Blotting, Western; Cysteine; Ferric Compounds; Glyceraldehyde-3-Phosphate Dehydrogenases; Hemocyanins; Histidine; Kidney; Kidney Neoplasms; Lysine; Male; Metallothionein; Nitrilotriacetic Acid; Rats; Rats, Wistar; Specific Pathogen-Free Organisms; Superoxide Dismutase | 2005 |
High-affinity adaptors for switchable recognition of histidine-tagged proteins.
Topics: Calorimetry; Chelating Agents; Fluorescein; Histidine; Humans; Kinetics; Membrane Proteins; Nickel; Nitrilotriacetic Acid; Protein Binding; Receptor, Interferon alpha-beta; Receptors, Interferon; Thermodynamics | 2005 |
A surface plasmon resonance-based assay for small molecule inhibitors of human cyclophilin A.
Topics: Binding, Competitive; Biosensing Techniques; Cyclophilin A; Cyclosporine; Enzyme Inhibitors; Enzyme Stability; Enzymes, Immobilized; Fluorescence; Histidine; Humans; Kinetics; Ligands; Models, Molecular; Molecular Weight; Nitrilotriacetic Acid; Recombinant Proteins; Solutions; Static Electricity; Surface Plasmon Resonance; Time Factors; Titrimetry | 2005 |
Preparation and characterization of nitrilotriacetic-acid-terminated self-assembled monolayers on gold surfaces for matrix-assisted laser desorption ionization-time of flight-mass spectrometry analysis of proteins and peptides.
Topics: Gold; Histidine; Nitrilotriacetic Acid; Peptides; Proteins; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Surface Properties | 2005 |
The novel chelator lipid 3(nitrilotriacetic acid)-ditetradecylamine (NTA(3)-DTDA) promotes stable binding of His-tagged proteins to liposomal membranes: potent anti-tumor responses induced by simultaneously targeting antigen, cytokine and costimulatory si
Topics: Amines; Animals; Antigens; B7-1 Antigen; Biosensing Techniques; CD40 Antigens; Cell Membrane; Cell Proliferation; Chelating Agents; Cytokines; Dose-Response Relationship, Drug; Female; Histidine; Interleukin-12; Interleukin-2; Kinetics; Lipids; Liposomes; Lymphocyte Activation; Male; Mice; Mice, Inbred DBA; Models, Chemical; Neoplasm Transplantation; Nitrilotriacetic Acid; Protein Binding; Recombinant Proteins; T-Lymphocytes; Temperature; Time Factors; Transfection | 2005 |
Synthesis of an artificial cell surface receptor that enables oligohistidine affinity tags to function as metal-dependent cell-penetrating peptides.
Topics: Acetates; Cell Membrane; Cholesterol; Green Fluorescent Proteins; Histidine; Humans; Jurkat Cells; Kinetics; Metals; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Receptors, Cell Surface; T-Lymphocytes | 2006 |
Selective labeling of extracellular proteins containing polyhistidine sequences by a fluorescein-nitrilotriacetic acid conjugate.
Topics: Fluoresceins; Fluorescent Dyes; HeLa Cells; Histidine; Humans; Nickel; Nitrilotriacetic Acid; Photochemistry; Proteins; Transfection | 2006 |
Purification of His-tagged hybrid phage antibody.
Topics: Animals; Antibodies, Monoclonal; Bacteriophages; Chromatography, Affinity; Genes, bcl-1; Histidine; Immunoglobulin Variable Region; Mice; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Library | 2006 |
Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation.
Topics: Chelating Agents; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Histidine; Interferon-alpha; Kinetics; Membrane Proteins; Models, Molecular; Nitrilotriacetic Acid; Oligopeptides; Photochemistry; Receptor, Interferon alpha-beta; Receptors, Interferon; Spectrometry, Fluorescence; Substrate Specificity | 2006 |
An evaluation of in vitro protein-protein interaction techniques: assessing contaminating background proteins.
Topics: Blotting, Far-Western; Chromatography, Affinity; Drug Contamination; Histidine; Nitrilotriacetic Acid; Organometallic Compounds; Protein Array Analysis; Protein Interaction Mapping; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2006 |
Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle.
Topics: Coated Materials, Biocompatible; Histidine; Magnetics; Micelles; Motion; Nanostructures; Nitrilotriacetic Acid; Phospholipids; Recombinant Proteins | 2006 |
Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces.
Topics: Binding Sites; Chromatography, Affinity; Enzyme-Linked Immunosorbent Assay; Green Fluorescent Proteins; Histidine; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Protein Array Analysis; Sensitivity and Specificity; Sepharose; Surface Properties; Time Factors | 2006 |
Controlling the activity of the 20S proteasome complex by synthetic gatekeepers.
Topics: Archaeal Proteins; Caseins; Chromatography, Gel; Endopeptidases; Enzyme Stability; Enzymes, Immobilized; Fluorescein; Fluorescent Dyes; Histidine; Models, Molecular; Nitrilotriacetic Acid; Protein Structure, Secondary; Substrate Specificity; Thermoplasma | 2006 |
Differential protein assembly on micropatterned surfaces with tailored molecular and surface multivalency.
Topics: Chelating Agents; Histidine; Imidazoles; Interferon-alpha; Ligands; Membrane Proteins; Nickel; Nitrilotriacetic Acid; Polyethylene Glycols; Protein Array Analysis; Protein Binding; Proteins; Receptor, Interferon alpha-beta; Receptors, Interferon; Recombinant Fusion Proteins; Sulfhydryl Compounds; Surface Properties | 2006 |
Cyanobacterial small chlorophyll-binding protein ScpD (HliB) is located on the periphery of photosystem II in the vicinity of PsbH and CP47 subunits.
Topics: Bacterial Proteins; Crystallography, X-Ray; Cyanobacteria; Dimerization; Epitopes; Histidine; Immunohistochemistry; Light-Harvesting Protein Complexes; Models, Molecular; Mutation; Nickel; Nitrilotriacetic Acid; Photosystem II Protein Complex; Protein Subunits; Synechocystis; Thylakoids | 2006 |
Preparation of nitrilotriacetic acid/Co2+-linked, silica/boron-coated magnetite nanoparticles for purification of 6 x histidine-tagged proteins.
Topics: Cobalt; Electrophoresis, Polyacrylamide Gel; Ferrosoferric Oxide; Histidine; Metal Nanoparticles; Microscopy, Electron, Transmission; Nitrilotriacetic Acid; Proteins; Silicon Dioxide | 2007 |
NTA-mediated protein capturing strategy in screening experiments for small organic molecules by surface plasmon resonance.
Topics: Binding Sites; Biosensing Techniques; Histidine; Humans; Nitrilotriacetic Acid; Peptides; Phosphatidylinositol 3-Kinases; src Homology Domains; Surface Plasmon Resonance | 2007 |
Site-specific fluorescent labeling of poly-histidine sequences using a metal-chelating cysteine.
Topics: Binding Sites; Bridged Bicyclo Compounds; Chelating Agents; Cross-Linking Reagents; Cysteine; Fluorescent Dyes; Histidine; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Spectrometry, Fluorescence; Staining and Labeling; Substrate Specificity | 2007 |
Specific adsorption of histidine-tagged proteins on silica surfaces modified with Ni2+/NTA-derivatized poly(ethylene glycol).
Topics: Adsorption; Biosensing Techniques; Histidine; In Vitro Techniques; Lipid Bilayers; Molecular Structure; Nickel; Nitrilotriacetic Acid; Polyethylene Glycols; Proteins; Silicon Dioxide; Surface Properties | 2007 |
Creating nanopatterns of His-tagged proteins on surfaces by nanoimprint lithography using specific NiNTA-histidine interactions.
Topics: Coated Materials, Biocompatible; Crystallization; Histidine; Macromolecular Substances; Materials Testing; Molecular Conformation; Nanostructures; Nanotechnology; Nickel; Nitrilotriacetic Acid; Particle Size; Proteins; Staining and Labeling; Surface Properties | 2007 |
Determination of the rate constants for the FK506 binding protein/rapamycin interaction using surface plasmon resonance: an alternative sensor surface for Ni2+-nitrilotriacetic acid immobilization of His-tagged proteins.
Topics: Histidine; Humans; Kinetics; Nickel; Nitrilotriacetic Acid; Recombinant Proteins; Sirolimus; Surface Plasmon Resonance; Tacrolimus Binding Proteins | 2007 |
Nitrilotriacetic acid-coated magnetic nanoparticles as affinity probes for enrichment of histidine-tagged proteins and phosphorylated peptides.
Topics: Affinity Labels; Amino Acid Sequence; Histidine; Magnetics; Molecular Sequence Data; Nanoparticles; Nitrilotriacetic Acid; Peptides; Phosphorylation; Proteins; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2007 |
High-capacity purification of His-tagged proteins by affinity membranes containing functionalized polymer brushes.
Topics: Adsorption; Aluminum; Gold; Histidine; Membranes, Artificial; Methacrylates; Models, Chemical; Nickel; Nitrilotriacetic Acid; Polyhydroxyethyl Methacrylate; Polymers; Protein Engineering; Proteins; Silver Staining | 2007 |
Dual-mode fluorophore-doped nickel nitrilotriacetic acid-modified silica nanoparticles combine histidine-tagged protein purification with site-specific fluorophore labeling.
Topics: Absorptiometry, Photon; Estrogen Receptor alpha; Fluorescent Dyes; Glass; Histidine; Ligands; Models, Molecular; Molecular Structure; Nanoparticles; Nitrilotriacetic Acid; Nuclear Magnetic Resonance, Biomolecular; Organometallic Compounds; Particle Size; Photochemistry; Protein Array Analysis; Silicon Dioxide; Solutions; Time Factors | 2007 |
Noncovalent, site-specific biotinylation of histidine-tagged proteins.
Topics: Biotinylation; Carrier Proteins; Histidine; Maltose-Binding Proteins; Models, Molecular; Molecular Structure; Nitrilotriacetic Acid; Protein Array Analysis; Protein Binding; Solutions; Surface Plasmon Resonance | 2007 |
Gold nanoparticle size controlled by polymeric Au(I) thiolate precursor size.
Topics: Adenoviridae; Chlorides; Glutathione; Gold; Gold Compounds; Histidine; Metal Nanoparticles; Nitrilotriacetic Acid; Organometallic Compounds; Particle Size; Polymers; Protein Conformation; Viral Proteins | 2008 |
Anchoring of histidine-tagged proteins to molecular printboards: self-assembly, thermodynamic modeling, and patterning.
Topics: Adsorption; beta-Cyclodextrins; Binding Sites; Carrier Proteins; Histidine; Luminescent Proteins; Maltose-Binding Proteins; Models, Chemical; Molecular Conformation; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Particle Size; Proteasome Endopeptidase Complex; Surface Plasmon Resonance; Surface Properties; Thermodynamics; Time Factors | 2008 |
A polymeric molecular "handle" for multiple AFM-based single-molecule force measurements.
Topics: Chelating Agents; Histidine; Microscopy, Atomic Force; Nanotechnology; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds | 2008 |
Metal-chelate affinity chromatography.
Topics: Animals; Base Sequence; Chelating Agents; Chromatography, Affinity; Cloning, Molecular; Dialysis; Genetic Vectors; Histidine; Humans; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Protein Denaturation; Protein Renaturation; Recombinant Fusion Proteins | 2001 |
Monolayer purification: a rapid method for isolating protein complexes for single-particle electron microscopy.
Topics: Adsorption; Animals; Cell Extracts; Chemistry Techniques, Analytical; Cryoelectron Microscopy; Histidine; Lipid Metabolism; Multiprotein Complexes; Nickel; Nitrilotriacetic Acid; Oligopeptides; Receptors, Transferrin; Ribosomal Protein L3; Ribosomal Proteins; Ribosomes; Transferrin | 2008 |
Ni-nitrilotriacetic acid-modified quantum dots as a site-specific labeling agent of histidine-tagged proteins in live cells.
Topics: Cell Line; Cell Survival; Fluorescent Dyes; Histidine; Humans; Nickel; Nitrilotriacetic Acid; Proteins; Quantum Dots; Staining and Labeling | 2008 |
Metal-chelate affinity chromatography.
Topics: Amino Acid Sequence; Base Sequence; Chromatography, Affinity; Genetic Vectors; Histidine; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Protein Denaturation; Protein Renaturation; Recombinant Fusion Proteins; Resins, Synthetic | 2001 |
Copper is taken up efficiently from albumin and alpha2-macroglobulin by cultured human cells by more than one mechanism.
Topics: alpha-Macroglobulins; Binding, Competitive; Breast Neoplasms; Cation Transport Proteins; Cell Line, Tumor; Copper; Copper Transporter 1; Female; Histidine; Humans; Ion Transport; Iron; Kinetics; Liver Neoplasms; Manganese; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Serum Albumin; Silver; Zinc | 2008 |
Conjugation of DNA with protein using His-tag chemistry and its application to the aptamer-based detection system.
Topics: Alkaline Phosphatase; Aptamers, Nucleotide; Chemistry Techniques, Analytical; DNA; Histidine; Nitrilotriacetic Acid; Protein Binding; Proteins; Recombinant Proteins; Reproducibility of Results | 2008 |
Nitrilotriacetic acid-derivatized quantum dots for simple purification and site-selective fluorescent labeling of active proteins in a single step.
Topics: Animals; Cadmium Compounds; Carboxylic Acids; Catalytic Domain; Fluorescent Dyes; Glutathione Transferase; Histidine; Models, Molecular; Molecular Conformation; Nickel; Nitrilotriacetic Acid; Protein Binding; Quantum Dots; Schistosoma; Selenium Compounds; Substrate Specificity; Sulfides; Surface Properties; Zinc Compounds | 2008 |
Immobilization of bacteriophage Qbeta on metal-derivatized surfaces via polyvalent display of hexahistidine tags.
Topics: Allolevivirus; Histidine; Metals; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Recombinant Fusion Proteins; Surface Plasmon Resonance | 2008 |
Nickel-ion-mediated control of the stoichiometry of his-tagged protein/nanoparticle interactions.
Topics: Electrophoresis, Agar Gel; Fluorescence; Green Fluorescent Proteins; Histidine; Ions; Kinetics; Ligands; Nanoparticles; Nickel; Nitrilotriacetic Acid; Oligopeptides; Sulfhydryl Compounds; Titrimetry | 2009 |
Positioning multiple proteins at the nanoscale with electron beam cross-linked functional polymers.
Topics: Binding Sites; Biotin; Histidine; Maleimides; Microscopy, Atomic Force; Microscopy, Fluorescence; Nanoparticles; Nanotechnology; Nitrilotriacetic Acid; Polyethylene Glycols; Proteins | 2009 |
High-affinity labeling and tracking of individual histidine-tagged proteins in live cells using Ni2+ tris-nitrilotriacetic acid quantum dot conjugates.
Topics: Biocompatible Materials; Cadmium Compounds; Cell Adhesion; Green Fluorescent Proteins; HeLa Cells; Histidine; Humans; Microscopy, Fluorescence; Nanotechnology; Nickel; Nitrilotriacetic Acid; Polyethylene Glycols; Quantum Dots; Receptors, Interferon; Selenium Compounds; Sulfides; Zinc Compounds | 2009 |
NTA directed protein nanopatterning on DNA Origami nanoconstructs.
Topics: Biosensing Techniques; DNA; Green Fluorescent Proteins; Histidine; Microscopy, Atomic Force; Nanostructures; Nitrilotriacetic Acid | 2009 |
Characterization of the electron transfer of a ferrocene redox probe and a histidine-tagged hemoprotein specifically bound to a nitrilotriacetic-terminated self-assembled monolayer.
Topics: Chelating Agents; Electron Transport; Ferrous Compounds; Hemeproteins; Histidine; Humans; Metallocenes; Molecular Structure; Nitrilotriacetic Acid; Oxidation-Reduction | 2009 |
A facile method for reversibly linking a recombinant protein to DNA.
Topics: DNA; Histidine; Nickel; Nitrilotriacetic Acid; Oligonucleotide Array Sequence Analysis; Oligonucleotides; Oligopeptides; Protein Binding; Recombinant Proteins | 2009 |
Selectivity of competitive multivalent interactions at interfaces.
Topics: Histidine; Kinetics; Ligands; Nitrilotriacetic Acid; Oligopeptides | 2009 |
Tris-nitrilotriacetic acids of subnanomolar affinity toward hexahistidine tagged molecules.
Topics: Biotin; Histidine; Molecular Structure; Nanostructures; Nitrilotriacetic Acid; Oligopeptides; Peptide Fragments; Stereoisomerism; Surface Plasmon Resonance | 2009 |
Accelerating phage-display library selection by reversible and site-specific biotinylation.
Topics: Adsorption; Amino Acid Sequence; Binding Sites; Biotinylation; Epitopes; Fibronectins; Fungal Proteins; Histidine; Immobilized Proteins; Models, Molecular; Molecular Sequence Data; Nitrilotriacetic Acid; Peptide Library; Protein Structure, Secondary; Substrate Specificity; SUMO-1 Protein | 2009 |
Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(II)-NTA probe.
Topics: Binding Sites; Coumarins; Fluorescent Dyes; Green Fluorescent Proteins; Histidine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Staining and Labeling | 2009 |
Insight into the complexation mode of bis(nitrilotriacetic acid) (NTA) ligands with Ni(2+) involved in the labeling of histidine-tagged proteins.
Topics: Calorimetry; Fluorescence Polarization; Histidine; Ligands; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Proteins; Staining and Labeling | 2009 |
Bisphosphonate adaptors for specific protein binding on zirconium phosphonate-based microarrays.
Topics: Archaeal Proteins; Binding Sites; Enzymes, Immobilized; Histidine; Membranes, Artificial; Muramidase; Nickel; Nitrilotriacetic Acid; Organophosphonates; Particle Size; Protein Array Analysis; Recombinant Proteins; Sulfolobus acidocaldarius; Surface Properties; Zirconium | 2009 |
Sequential processing from cell lysis to protein assay on a chip enabling the optimization of an F(1)-ATPase single molecule assay condition.
Topics: Bacillus; Biotin; Cell Extracts; Diffusion; Electrodes; Enzyme Assays; Escherichia coli; Histidine; Intracellular Space; Microspheres; Nitrilotriacetic Acid; Organometallic Compounds; Protein Array Analysis; Proton-Translocating ATPases; Streptavidin; Time Factors | 2009 |
Compact and versatile nickel-nitrilotriacetate-modified quantum dots for protein imaging and Förster resonance energy transfer based assay.
Topics: Cadmium Compounds; Cell Line; Fluorescence Resonance Energy Transfer; Histidine; Humans; Ligands; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Quantum Dots; Recombinant Proteins; Selenium Compounds; Solubility; Sulfides; Water; Zinc Compounds | 2010 |
Hexahistidine-tag-specific optical probes for analyses of proteins and their interactions.
Topics: Alkyl and Aryl Transferases; Carbocyanines; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Histidine; N-Ethylmaleimide-Sensitive Proteins; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Protein Interaction Domains and Motifs; Spectrometry, Fluorescence; Ultracentrifugation | 2010 |
Protein purification with polymeric affinity membranes containing functionalized poly(acid) brushes.
Topics: Binding Sites; Carrier Proteins; Chromatography, Affinity; Egg White; Histidine; Humans; Membranes, Artificial; Muramidase; Nickel; Nitrilotriacetic Acid; Nylons; Polymers; Protein Binding; Spectroscopy, Fourier Transform Infrared; Ubiquitin | 2010 |
In situ assembly of macromolecular complexes triggered by light.
Topics: Chelating Agents; Chromatography, Gel; Histidine; Light; Maltose-Binding Proteins; Molecular Structure; Nitrilotriacetic Acid; Oligopeptides; Periplasmic Binding Proteins; Protein Binding; Protein Conformation; Receptors, LDL; Receptors, Virus; Rhinovirus; Virion | 2010 |
Low pH-triggered model drug molecule release from virus-like particles.
Topics: Animals; Capsid Proteins; Coloring Agents; Drug Carriers; Fluorescent Dyes; Histidine; Hydrogen-Ion Concentration; Mice; Models, Molecular; Nanoparticles; NIH 3T3 Cells; Nitrilotriacetic Acid; Oligopeptides; Pharmaceutical Preparations; Rhodamines | 2010 |
Protein-resistant NTA-functionalized polymer brushes for selective and stable immobilization of histidine-tagged proteins.
Topics: Biosensing Techniques; Catalysis; Histidine; Immobilized Proteins; Microarray Analysis; Nitrilotriacetic Acid; Polyethylene Glycols; Polyhydroxyethyl Methacrylate; Recombinant Fusion Proteins | 2010 |
Influence of multivalent nitrilotriacetic acid lipid-ligand affinity on the circulation half-life in mice of a liposome-attached His6-protein.
Topics: Animals; Half-Life; Histidine; Ligands; Lipids; Liposomes; Mice; Mice, Inbred BALB C; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Proteins | 2010 |
Native laser lithography of His-tagged proteins by uncaging of multivalent chelators.
Topics: Chelating Agents; Histidine; Lasers; Nanostructures; Nitrilotriacetic Acid; Peptides; Printing; Recombinant Proteins; Ultraviolet Rays | 2010 |
Bifunctional polyacrylamide based polymers for the specific binding of hexahistidine tagged proteins on gold surfaces.
Topics: Acrylic Resins; Animals; Cattle; Gold; Histidine; Metal Nanoparticles; Nitrilotriacetic Acid; Oligopeptides; Photoelectron Spectroscopy; Polymers; Recombinant Fusion Proteins; Serum Albumin, Bovine; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Surface Plasmon Resonance; Surface Properties | 2010 |
Specific and reversible immobilization of histidine-tagged proteins on functionalized silicon nanowires.
Topics: Biosensing Techniques; Histidine; Immobilized Proteins; Nanowires; Nickel; Nitrilotriacetic Acid; Proteins; Silicon | 2010 |
Immobilization of His-tagged endoglucanase on gold via various Ni-NTA self-assembled monolayers and its hydrolytic activity.
Topics: Biosensing Techniques; Cellulase; Enzymes, Immobilized; Gold; Histidine; Hydrolysis; Indicators and Reagents; Molecular Structure; Nitrilotriacetic Acid; Spectroscopy, Fourier Transform Infrared; Surface Properties; Thermodynamics | 2010 |
Synthesis of a pH-sensitive nitrilotriacetic linker to peptide transduction domains to enable intracellular delivery of histidine imidazole ring-containing macromolecules.
Topics: Animals; Cell Line, Tumor; Disease Models, Animal; Drug Carriers; Drug Delivery Systems; Gene Expression Regulation, Enzymologic; Histidine; Humans; Hydrogen-Ion Concentration; Imidazoles; Luciferases; Macromolecular Substances; Mice; Mice, Transgenic; Nitrilotriacetic Acid; Organometallic Compounds; Peptides | 2010 |
Creating biomimetic surfaces through covalent and oriented binding of proteins.
Topics: Animals; Biomimetics; Cadherins; Cell Adhesion; Cell Line; Chelating Agents; Glass; Histidine; Hydrogen-Ion Concentration; Immobilized Proteins; Metals; Mice; Nitrilotriacetic Acid; Oligopeptides; Osmolar Concentration; Protein Binding; Silanes; Surface Properties | 2010 |
Activation of human VPS4A by ESCRT-III proteins reveals ability of substrates to relieve enzyme autoinhibition.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Motifs; Amino Acid Sequence; ATPases Associated with Diverse Cellular Activities; Binding Sites; Endosomal Sorting Complexes Required for Transport; Enzyme Activation; Histidine; Humans; Hydrolysis; Liposomes; Molecular Sequence Data; Mutation; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Protein Multimerization; Substrate Specificity; Vacuolar Proton-Translocating ATPases | 2010 |
Localized cell uptake of His-tagged polyplexes immobilized on NTA self-assembled monolayers.
Topics: Animals; Edetic Acid; Flow Cytometry; Histidine; Imidazoles; Mice; NIH 3T3 Cells; Nitrilotriacetic Acid; Organometallic Compounds; Surface Plasmon Resonance | 2010 |
Site-specific biomolecule labeling with gold clusters.
Topics: Cryoelectron Microscopy; Gold; Histidine; Metal Nanoparticles; Nitrilotriacetic Acid; Proteins | 2010 |
Controlled stoichiometric synthesis of DNA-quantum dot conjugates using Ni-mediated coordination chemistry.
Topics: Fluorescence Resonance Energy Transfer; Histidine; Nickel; Nitrilotriacetic Acid; Oligonucleotides; Quantum Dots | 2010 |
Construction of a 'turn-on' fluorescent probe system for His-tagged proteins.
Topics: Binding Sites; Fluorescent Dyes; Histidine; Molecular Probe Techniques; Nitrilotriacetic Acid; Oligopeptides; p-Dimethylaminoazobenzene; Proteins; Spectrum Analysis | 2010 |
Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes.
Topics: Coloring Agents; DNA; DNA Mismatch Repair; Energy Transfer; Fluorescence Resonance Energy Transfer; Histidine; Macromolecular Substances; MutS DNA Mismatch-Binding Protein; Nitrilotriacetic Acid; Nucleic Acid Conformation; Protein Conformation; SNARE Proteins; Tromethamine | 2010 |
Antibody response to polyhistidine-tagged peptide and protein antigens attached to liposomes via lipid-linked nitrilotriacetic acid in mice.
Topics: Adjuvants, Immunologic; Animals; Antibodies; Antigens; Female; Histidine; HIV Envelope Protein gp41; Liposomes; Mice; Mice, Inbred BALB C; Nitrilotriacetic Acid; Ovalbumin; Peptides; Protein Binding; Vaccines, Subunit | 2011 |
Ultrasensitive scanometric strategy for detection of matrix metalloproteinases using a histidine tagged peptide-Au nanoparticle probe.
Topics: Amino Acid Sequence; Biosensing Techniques; Gold; Histidine; Matrix Metalloproteinase 7; Matrix Metalloproteinases; Metal Nanoparticles; Molecular Sequence Data; Nitrilotriacetic Acid; Peptides; Substrate Specificity | 2011 |
Development of a metal-chelated plasmonic interface for the linking of His-peptides with a droplet-based surface plasmon resonance read-off scheme.
Topics: Amines; Amino Acid Sequence; Bradykinin; Chelating Agents; Histidine; Immobilized Proteins; Metals; Molecular Sequence Data; Nitrilotriacetic Acid; Peptides; Surface Plasmon Resonance | 2011 |
Multivalent and flexible PEG-nitrilotriacetic acid derivatives for non-covalent protein pegylation.
Topics: Animals; Chelating Agents; Chromatography, Affinity; Coordination Complexes; Drug Delivery Systems; Granulocyte Colony-Stimulating Factor; Histidine; Male; Nitrilotriacetic Acid; Polyethylene Glycols; Protein Binding; Protein Structure, Secondary; Proteins; Rats; Rats, Sprague-Dawley; Surface Plasmon Resonance | 2011 |
A gold nanoparticle platform for protein-protein interactions and drug discovery.
Topics: Drug Evaluation; Gold; High-Throughput Screening Assays; Histidine; Metal Nanoparticles; Mucin-1; Nitrilotriacetic Acid; Oligopeptides; Protein Interaction Mapping; Proteins | 2011 |
Microgels for efficient protein purification.
Topics: Chelating Agents; Chromatography, Affinity; Ferritins; Gels; Histidine; Humans; Metals; Nitrilotriacetic Acid | 2011 |
Overexpression of (His)6-tagged human arginase I in Saccharomyces cerevisiae and enzyme purification using metal affinity chromatography.
Topics: Arginase; Chromatography, Affinity; Cloning, Molecular; Histidine; Humans; Liver; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Recombinant Fusion Proteins; Saccharomyces cerevisiae | 2012 |
Reversible major histocompatibility complex I-peptide multimers containing Ni(2+)-nitrilotriacetic acid peptides and histidine tags improve analysis and sorting of CD8(+) T cells.
Topics: CD8-Positive T-Lymphocytes; Histidine; HLA-A2 Antigen; Humans; MART-1 Antigen; Nickel; Nitrilotriacetic Acid; Peptides; Staining and Labeling | 2011 |
Development of a histidine-targeted spectrophotometric sensor using Ni(II)NTA-functionalized Au and Ag nanoparticles.
Topics: Amino Acid Sequence; Antigens, Protozoan; Colloids; Gold; Histidine; Humans; Hydrogen-Ion Concentration; Malaria, Falciparum; Metal Nanoparticles; Microscopy, Electron, Transmission; Molecular Mimicry; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Peptides; Plasmodium falciparum; Protozoan Proteins; Reagent Kits, Diagnostic; Silver; Spectrophotometry | 2011 |
Specifically and reversibly immobilizing proteins/enzymes to nitriolotriacetic-acid-modified mesoporous silicas through histidine tags for purification or catalysis.
Topics: Alkyl and Aryl Transferases; Catalysis; Histidine; Horseradish Peroxidase; Hydrogen-Ion Concentration; Immobilized Proteins; Nitrilotriacetic Acid; Porosity; Silicon Dioxide | 2011 |
Aptameric peptide for one-step detection of protein kinase.
Topics: 1-Methyl-3-isobutylxanthine; Aptamers, Peptide; Biosensing Techniques; Cell Line, Tumor; Colforsin; Cyclic AMP-Dependent Protein Kinases; Dielectric Spectroscopy; Electrodes; Gold; Histidine; Humans; Immobilized Proteins; Kinetics; Limit of Detection; Nitrilotriacetic Acid; Oligopeptides; Protein Kinase Inhibitors; Quartz Crystal Microbalance Techniques | 2012 |
Surface-attached polyhistidine-tag proteins characterized by FTIR difference spectroscopy.
Topics: Germanium; Histidine; Immobilized Proteins; Lipid Bilayers; Lipids; Nitrilotriacetic Acid; Proteins; Spectroscopy, Fourier Transform Infrared; Surface Properties | 2012 |
Affinity binding via zinc(II) for controlled orientation and electrochemistry of histidine-tagged nitrate reductase in self-assembled monolayers.
Topics: Animals; Aspergillus niger; Carbon; Cattle; Copper; Electrochemistry; Electrodes; Enzymes, Immobilized; Glass; Histidine; Nitrate Reductase; Nitrilotriacetic Acid; Paraquat; Zinc | 2013 |
Development of a novel optical biosensor for detection of organophosphorus pesticides based on methyl parathion hydrolase immobilized by metal-chelate affinity.
Topics: Biosensing Techniques; Chelating Agents; Chromatography, Affinity; Enzymes, Immobilized; Histidine; Metals; Methyl Parathion; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organophosphorus Compounds; Pesticides; Phosphoric Monoester Hydrolases; Recombinant Proteins | 2012 |
pH responsive PEGylation through metal affinity for gene delivery mediated by histidine-grafted polyethylenimine.
Topics: Animals; Biocompatible Materials; Cattle; Chelating Agents; CHO Cells; Coated Materials, Biocompatible; Cricetinae; Cricetulus; Gene Transfer Techniques; Histidine; Hydrogen-Ion Concentration; Materials Testing; Nickel; Nitrilotriacetic Acid; Plasmids; Polyethylene Glycols; Polyethyleneimine; Transfection; Zinc | 2013 |
Cobalt(III) as a stable and inert mediator ion between NTA and His6-tagged proteins.
Topics: Cobalt; Green Fluorescent Proteins; Histidine; Immobilized Proteins; Ions; Kinetics; Nitrilotriacetic Acid; Oligopeptides; Proteins | 2013 |
A His-tagged Melanocarpus albomyces laccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films.
Topics: Aniline Compounds; Ascomycota; Carbon; Diazonium Compounds; Electric Conductivity; Electrochemical Techniques; Electrodes; Histidine; Laccase; Nitrilotriacetic Acid; Oxidation-Reduction; Oxygen; Polyvinyls; Surface Properties | 2013 |
Dual functionalized graphene oxide serves as a carrier for delivering oligohistidine- and biotin-tagged biomolecules into cells.
Topics: Avidin; Biotin; Cell Line, Tumor; Drug Carriers; Epithelial Cells; Fluorescent Dyes; Genes, Reporter; Graphite; Green Fluorescent Proteins; Histidine; Humans; Microscopy, Atomic Force; Microscopy, Fluorescence; Nanoparticles; Nitrilotriacetic Acid; Oligopeptides; Oxides; Spectroscopy, Fourier Transform Infrared; Staining and Labeling; Tromethamine | 2013 |
Immobilization of enzymes on magnetic beads through affinity interactions.
Topics: Chelating Agents; Enzymes, Immobilized; Histidine; Magnetic Phenomena; Magnetite Nanoparticles; Nickel; Nitrilotriacetic Acid; Oligopeptides; Plant Lectins; Protein Binding; Streptavidin | 2013 |
Label-free fluorescent detection of thrombin activity based on a recombinant enhanced green fluorescence protein and nickel ions immobilized nitrilotriacetic acid-coated magnetic nanoparticles.
Topics: Antithrombins; Equipment Reuse; Green Fluorescent Proteins; Hirudins; Histidine; Humans; Immobilized Proteins; Limit of Detection; Magnetite Nanoparticles; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Proteolysis; Recombinant Fusion Proteins; Reproducibility of Results; Spectrometry, Fluorescence; Thrombin | 2013 |
Thermodynamics of imidazole-ligand binding to Ni-nitrilotriacetate in solution and covalently attached to agarose beads: imidazole, his-6 (his-tag) peptide and a new bis-imidazolo-dithiane.
Topics: Calorimetry; Chromatography, Affinity; Histidine; Imidazoles; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Protein Binding; Thermodynamics | 2014 |
High-fidelity protein targeting into membrane lipid microdomains in living cells.
Topics: Acetates; Diffusion; HeLa Cells; Histidine; Humans; Maltose-Binding Proteins; Membrane Microdomains; Nitrilotriacetic Acid; Nitro Compounds; Oligopeptides; Protein Binding; Proteins; Recombinant Fusion Proteins; Unilamellar Liposomes | 2014 |
Ubiquitination of Rac1 by inhibitors of apoptosis (IAPs).
Topics: HEK293 Cells; HeLa Cells; Histidine; Humans; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; rac1 GTP-Binding Protein; Ubiquitination; X-Linked Inhibitor of Apoptosis Protein | 2014 |
High-affinity gold nanoparticle pin to label and localize histidine-tagged protein in macromolecular assemblies.
Topics: Adenosine Triphosphatases; Bacterial Proteins; Escherichia coli; Gold; Histidine; Indicators and Reagents; Metal Nanoparticles; Microscopy, Electron; Nitrilotriacetic Acid; Recombinant Fusion Proteins; Ribosome Subunits, Large, Bacterial; Staining and Labeling; Thermus thermophilus; Tromethamine | 2014 |
Localizing chemical groups while imaging single native proteins by high-resolution atomic force microscopy.
Topics: Binding Sites; Histidine; Inorganic Chemicals; Ligands; Microscopy, Atomic Force; Molecular Imaging; Nanotechnology; Nitrilotriacetic Acid; Organometallic Compounds; Proteins | 2014 |
An electrochemical peptide-based Ara h 2 antibody sensor fabricated on a nickel(II)-nitriloacetic acid self-assembled monolayer using a His-tagged peptide.
Topics: Antibodies; Biosensing Techniques; Electrochemical Techniques; Histidine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Peptides | 2014 |
A supramolecular host-guest carrier system for growth factors employing V(H)H fragments.
Topics: beta-Cyclodextrins; Bone Morphogenetic Protein 6; Histidine; Humans; Kinetics; Molecular Structure; Nitrilotriacetic Acid; Particle Size; Single-Domain Antibodies; Surface Properties; Thermodynamics | 2014 |
Live-cell targeting of his-tagged proteins by multivalent N-nitrilotriacetic acid carrier complexes.
Topics: Amino Acid Sequence; Cell Survival; HeLa Cells; Histidine; Humans; Kinetics; Nitrilotriacetic Acid; Peptide Fragments; Recombinant Proteins; Staining and Labeling; tat Gene Products, Human Immunodeficiency Virus | 2014 |
Hybridization of an Aβ-specific antibody fragment with aminopyrazole-based β-sheet ligands displays striking enhancement of target affinity.
Topics: Alzheimer Disease; Amyloid beta-Peptides; Catalysis; Enzyme-Linked Immunosorbent Assay; Epitopes; Histidine; Humans; Hydrogen; Hydrogen-Ion Concentration; Immunoglobulin Fragments; Immunotherapy; Ligands; Magnetic Resonance Spectroscopy; Nickel; Nitrilotriacetic Acid; Peptide Fragments; Protein Structure, Secondary; Protein Structure, Tertiary; Pyrazoles; Recombinant Proteins; Single-Chain Antibodies | 2015 |
A dual-responsive pH-sensor and its potential as a universal probe for assays of pH-changing enzymes.
Topics: Bacteria; beta-Lactamases; Cobalt; Enzyme Assays; Histidine; Hydrogen-Ion Concentration; Nitrilotriacetic Acid | 2015 |
In vitro systems for the study of microtubule-based cell polarity in fission yeast.
Topics: Actin Cytoskeleton; Cell Membrane; Cell Polarity; Chromatography, Affinity; Histidine; Microfluidics; Microtubule-Associated Proteins; Microtubules; Nickel; Nitrilotriacetic Acid; Oligopeptides; Phosphoprotein Phosphatases; Phosphorylation; Protein Binding; Protein Kinases; Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Spindle Pole Bodies | 2015 |
His-tagged norovirus-like particles: A versatile platform for cellular delivery and surface display.
Topics: Animals; Biotechnology; Capsid Proteins; Cell-Penetrating Peptides; Drug Carriers; Epitopes; HEK293 Cells; Histidine; Humans; Nitrilotriacetic Acid; Norovirus; Sf9 Cells; Technology, Pharmaceutical; Vaccines, Virus-Like Particle; Viral Vaccines | 2015 |
Controlled immobilization of His-tagged proteins for protein-ligand interaction experiments using Ni²⁺-NTA layer on glass surfaces.
Topics: Biosensing Techniques; Glass; Histidine; Ligands; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; S100 Proteins | 2015 |
Metallochelate Coupling of Phosphorescent Pt-Porphyrins to Peptides, Proteins, and Self-Assembling Protein Nanoparticles.
Topics: Animals; Biosensing Techniques; Cell Line; Histidine; Luminescent Agents; Luminescent Measurements; Metalloporphyrins; Mice; Nanoparticles; Nitrilotriacetic Acid; Oxygen; Peptides; Platinum; Proteins; Staining and Labeling | 2016 |
Nonfouling NTA-PEG-Based TEM Grid Coatings for Selective Capture of Histidine-Tagged Protein Targets from Cell Lysates.
Topics: Adsorption; ATP-Binding Cassette Transporters; Bacteriophage T7; Cell Extracts; Cryoelectron Microscopy; Escherichia coli; Escherichia coli Proteins; Gene Expression; Green Fluorescent Proteins; Histidine; Microscopy, Electron, Transmission; Nitrilotriacetic Acid; Oligopeptides; Phosphatidylethanolamines; Polyethylene Glycols; Recombinant Fusion Proteins; Ribosomal Proteins; Surface Properties | 2016 |
Cobalt(III)-Mediated Permanent and Stable Immobilization of Histidine-Tagged Proteins on NTA-Functionalized Surfaces.
Topics: Cobalt; Histidine; Immobilized Proteins; Kinetics; Nitrilotriacetic Acid; Proteins; Surface Properties | 2016 |
Fabrication of Nanometer- and Micrometer-Scale Protein Structures by Site-Specific Immobilization of Histidine-Tagged Proteins to Aminosiloxane Films with Photoremovable Protein-Resistant Protecting Groups.
Topics: Binding Sites; Histidine; Immobilized Proteins; Microtechnology; Nanostructures; Nitrilotriacetic Acid; Photochemical Processes; Siloxanes | 2016 |
Self-assembly of Ni-NTA-modified β-annulus peptides into artificial viral capsids and encapsulation of His-tagged proteins.
Topics: Capsid Proteins; Green Fluorescent Proteins; Histidine; Nickel; Nitrilotriacetic Acid; Peptides | 2016 |
Development of a Small Hybrid Molecule That Mediates Degradation of His-Tag Fused Proteins.
Topics: Biotin; Cell Line; Cysteine; Histidine; Humans; Leupeptins; Maleimides; Nitrilotriacetic Acid; Organometallic Compounds; Proteasome Inhibitors; Proteolysis; Receptors, Retinoic Acid; Recombinant Fusion Proteins; Smad2 Protein; Ubiquitin-Protein Ligases | 2018 |
Nitrilotriacetic Amine-Functionalized Polymeric Core-Shell Nanoparticles as Enzyme Immobilization Supports.
Topics: Amines; Enzymes, Immobilized; Histidine; Nanoparticles; Nitrilotriacetic Acid; Polymers | 2017 |
Simple and efficient knockdown of His-tagged proteins by ternary molecules consisting of a His-tag ligand, a ubiquitin ligase ligand, and a cell-penetrating peptide.
Topics: Cell Line; Cell-Penetrating Peptides; Dose-Response Relationship, Drug; Histidine; Humans; Leucine; Ligands; Molecular Structure; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Receptors, Retinoic Acid; Structure-Activity Relationship; Ubiquitin-Protein Ligases | 2017 |
Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags.
Topics: Animals; Histidine; Immobilized Proteins; Luminescent Proteins; Molecular Dynamics Simulation; Nickel; Nitrilotriacetic Acid; Oligopeptides; Red Fluorescent Protein; Sea Anemones; Surface Properties | 2017 |
Orthogonal supramolecular protein assembly on patterned bifunctional surfaces.
Topics: Adamantane; beta-Cyclodextrins; Green Fluorescent Proteins; Histidine; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Surface Properties | 2018 |
The Scaffold Design of Trivalent Chelator Heads Dictates Affinity and Stability for Labeling His-tagged Proteins in vitro and in Cells.
Topics: Chelating Agents; Cyclams; Fluorescent Dyes; HeLa Cells; Heterocyclic Compounds; Histidine; Humans; Kinetics; Maltose-Binding Proteins; Microscopy, Fluorescence; Nitrilotriacetic Acid; Oligopeptides; Protein Binding; Proteins | 2018 |
Orientation Controlled Protein Nanocapsules by Enzymatic Removal of a Polymer Template.
Topics: Green Fluorescent Proteins; Histidine; Immobilized Proteins; Nanocapsules; Nickel; Nitrilotriacetic Acid; Polymers; Surface Properties | 2018 |
A facile method to oriented immobilization of His-tagged BirA on Co
Topics: Biotinylation; Carbon-Nitrogen Ligases; Catalysis; Cobalt; Coordination Complexes; Enzymes, Immobilized; Escherichia coli; Escherichia coli Proteins; Histidine; Hydrogen Peroxide; Microspheres; Nitrilotriacetic Acid; Repressor Proteins; Sepharose | 2019 |
Covalent affixation of histidine-tagged proteins tethered onto Ni-nitrilotriacetic acid sensors for enhanced surface plasmon resonance detection of small molecule drugs and kinetic studies of antibody/antigen interactions.
Topics: Antigen-Antibody Reactions; Antigens; Carbonic Anhydrase II; Dextrans; Histidine; Humans; Immunoglobulin Fc Fragments; Kinetics; Limit of Detection; Nickel; Nitrilotriacetic Acid; Pharmaceutical Preparations; Sulfonamides; Surface Plasmon Resonance | 2019 |
Intracellular Delivery of His-Tagged Genome-Editing Proteins Enabled by Nitrilotriacetic Acid-Containing Lipidoid Nanoparticles.
Topics: Cell Line; Cell Survival; CRISPR-Cas Systems; Gene Editing; Green Fluorescent Proteins; Histidine; Humans; Lipids; Nanoparticles; Nitrilotriacetic Acid; Oligopeptides; Transfection | 2019 |
Green Fluorescent Probe for Imaging His
Topics: Cell Survival; Escherichia coli; Fluorescent Dyes; HeLa Cells; Histidine; Humans; Intracellular Space; Nickel; Nitrilotriacetic Acid; Optical Imaging; Proteins; Solubility; Xeroderma Pigmentosum Group A Protein | 2019 |
Probing Protein-Protein Interactions with Label-Free Mass Spectrometry Quantification in Combination with Affinity Purification by Spin-Tip Affinity Columns.
Topics: Chromatography, Affinity; Chromatography, High Pressure Liquid; Histidine; Humans; Microtubule-Associated Proteins; Nitrilotriacetic Acid; Oligopeptides; Peptides; Protein Binding; Protein Interaction Maps; Proteomics; Recombinant Fusion Proteins; Tandem Mass Spectrometry | 2020 |
Ultrafast in-gel detection by fluorescent super-chelator probes with HisQuick-PAGE.
Topics: Animals; Chelating Agents; Electrophoresis, Polyacrylamide Gel; Escherichia; Fluorescent Dyes; HeLa Cells; Histidine; Humans; Nitrilotriacetic Acid; Organometallic Compounds; Proteins; Sf9 Cells; Workflow | 2020 |
Double Histidine Based EPR Measurements at Physiological Temperatures Permit Site-Specific Elucidation of Hidden Dynamics in Enzymes.
Topics: Coordination Complexes; Copper; Electron Spin Resonance Spectroscopy; Glutathione Transferase; Histidine; Humans; Isoenzymes; Molecular Conformation; Molecular Dynamics Simulation; Nitrilotriacetic Acid; Temperature | 2020 |
Site-specific covalent labeling of His-tag fused proteins with N-acyl-N-alkyl sulfonamide reagent.
Topics: HEK293 Cells; Histidine; Humans; Indicators and Reagents; Lysine; Models, Molecular; Molecular Probes; Molecular Structure; Nickel; Nitrilotriacetic Acid; Proteins; Staining and Labeling; Sulfonamides | 2021 |
A general model to optimise Cu
Topics: Bacterial Proteins; Chelating Agents; Coordination Complexes; Copper; Electron Spin Resonance Spectroscopy; Histidine; Imino Acids; Models, Chemical; Nitrilotriacetic Acid; Spin Labels; Streptococcus | 2021 |
Pulse Dipolar EPR Reveals Double-Histidine Motif Cu
Topics: Bacterial Proteins; Calorimetry; Copper; Electron Spin Resonance Spectroscopy; Histidine; Hydrogen-Ion Concentration; Ions; Nitrilotriacetic Acid; Spin Labels; Streptococcus | 2021 |
Rapid and regenerable surface plasmon resonance determinations of biomarker concentration and biomolecular interaction based on tris-nitrilotriacetic acid chips.
Topics: Biomarkers; Histidine; Kinetics; Nitrilotriacetic Acid; Surface Plasmon Resonance | 2021 |
Tyrosine-Based Dual-Functional Interface for Trapping and On-Site Photo-Induced Covalent Immobilization of Proteins.
Topics: Histidine; Indicators and Reagents; Nickel; Nitrilotriacetic Acid; Tyrosine | 2022 |
Ligand Immobilization Methods for Affinity Chromatography.
Topics: Chromatography, Affinity; Histidine; Ligands; Nitrilotriacetic Acid | 2022 |
Development of a Modular NTA:His Tag Viral Vaccine for Co-delivery of Antigen and Adjuvant.
Topics: Adjuvants, Immunologic; Animals; Antigens; COVID-19; Histidine; Mice; Mice, Inbred C57BL; Nitrilotriacetic Acid; Ovalbumin; SARS-CoV-2; Viral Vaccines | 2023 |
Switch-on Fluorescence Analysis of Protease Activity with the Assistance of a Nickel Ion-Nitrilotriacetic Acid-Conjugated Magnetic Nanoparticle.
Topics: Fluorescence; Histidine; Magnetite Nanoparticles; Nickel; Nitrilotriacetic Acid; Peptide Hydrolases; Peptides | 2023 |