histidine has been researched along with isopropyl thiogalactoside in 22 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 0 (0.00) | 18.7374 |
| 1990's | 7 (31.82) | 18.2507 |
| 2000's | 9 (40.91) | 29.6817 |
| 2010's | 4 (18.18) | 24.3611 |
| 2020's | 2 (9.09) | 2.80 |
| Authors | Studies |
|---|---|
| Barritt, GJ; Chataway, TK | 1 |
| Baldwin, JE; John, P; Schofield, CJ; Thomas, P; Zhang, Z | 1 |
| Garg, LC; Mukhija, R; Pillai, D; Rupa, P | 1 |
| Aldema, ML; Levy, SB; McMurry, LM; Walmsley, AR | 1 |
| Appa Rao, KB; Garg, LC; Panda, AK; Totey, SM | 1 |
| Baron, T; Betemps, D; Cheynet, V; Mallet, F | 1 |
| Barry, JK; Maki, AH; Matthews, KS; Ozarowski, A | 1 |
| Im, H; Lee, HJ | 1 |
| Bao, Y; Curthoys, NP; Hamm, B; Kenny, J; Taylor, L; Toth, A; Wagers, B | 1 |
| Austin, CJ; Jamie, JF; Kosim-Satyaputra, P; Matin, A; Mizdrak, J; Parker, MW; Polekhina, G; Roberts, TH; Sirijovski, N; Truscott, RJ; Willows, RD | 1 |
| Jing, SR; Liu, NY; Ma, L; Wang, QR; Wang, XN; Zhou, MQ; Zou, QM | 1 |
| Fang, L; Hua, ZC; Jia, KZ; Ma, DY; Tang, YL; Yu, M | 1 |
| Darby, RA; Forde, GM; Hine, AV; Slater, NK | 1 |
| Cen, P; Fan, L; Gu, W; Huang, L; Ruan, H; Xu, Z | 1 |
| Cestele, S; Corzo, G; Estrada, G; Garcia, BI; Ortiz, E; Possani, LD; Schiavon, E; Wanke, E | 1 |
| de Pont, JJ; Gordon, E; Horsefield, R; Neutze, R; Snijder, A; Swarts, HG | 1 |
| Chen, PF; Dai, QM; Han, QW; Jin, HY; Wang, JJ; Xu, F; Xu, JM; Zhang, HY | 1 |
| Bae, JY; Do, KH; Jang, TH; Kim, B; Kim, BG; Park, HH; Park, OK; Yoon, JH; Yun, H | 1 |
| Abuhammad, A; Lack, N; Schweichler, J; Sim, E; Sim, RB; Staunton, D | 1 |
| Dassanayake, RS; Gunawardene, YI; Karunanayake, EH; Rodrigo, WW; Weerasena, OV | 1 |
| Bagheri, M; Khani, MH | 1 |
| Gyurcsik, B; Hunyadi-Gulyás, É; Nafaee, ZH | 1 |
22 other study(ies) available for histidine and isopropyl thiogalactoside
| Article | Year |
|---|---|
Purification of histidine-tagged ras and its use in the detection of ras binding proteins.
Topics: Animals; Brain; Carrier Proteins; Chromatography, Affinity; Cytosol; Dose-Response Relationship, Drug; Escherichia coli; Histidine; Hydrogen-Ion Concentration; Imidazoles; Isopropyl Thiogalactoside; Nickel; ras Proteins; Rats; Recombinant Fusion Proteins; Time Factors | 1995 |
Expression, purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from tomato in Escherichia coli.
Topics: Amino Acid Oxidoreductases; Base Sequence; Carbon Dioxide; Catalase; Cloning, Molecular; Diethyl Pyrocarbonate; Dithiothreitol; DNA, Complementary; Enzyme Induction; Escherichia coli; Histidine; Hydroxylamine; Hydroxylamines; Iron; Isopropyl Thiogalactoside; Molecular Sequence Data; Plant Proteins; Polymerase Chain Reaction; Recombinant Fusion Proteins; Serum Albumin, Bovine; Solanum lycopersicum; Stereoisomerism; Substrate Specificity; Transfection | 1995 |
High-level production and one-step purification of biologically active human growth hormone in Escherichia coli.
Topics: Animals; Chromatography, Affinity; DNA, Complementary; Escherichia coli; Gene Expression Regulation, Bacterial; Growth Hormone; Histidine; Humans; Inclusion Bodies; Isopropyl Thiogalactoside; Lymphoma; Mitogens; Peptides; Plasmids; Rats; Recombinant Fusion Proteins; Tumor Cells, Cultured | 1995 |
Purification of the Tn10-specified tetracycline efflux antiporter TetA in a native state as a polyhistidine fusion protein.
Topics: Antiporters; Bacterial Proteins; Base Sequence; Biological Transport; Cell Membrane; Circular Dichroism; Cloning, Molecular; Detergents; DNA Transposable Elements; Electrophoresis, Polyacrylamide Gel; Histidine; Isopropyl Thiogalactoside; Molecular Sequence Data; Peptides; Protein Binding; Protein Denaturation; Protein Structure, Secondary; Recombinant Fusion Proteins; Spectrometry, Fluorescence; Tetracycline Resistance; Tetracyclines | 1996 |
High-level expression of ovine growth hormone in Escherichia coli: single-step purification and characterization.
Topics: Amino Acid Sequence; Animals; Chromatography, Affinity; Cloning, Molecular; Escherichia coli; Gene Expression Regulation, Bacterial; Genetic Vectors; Growth Hormone; Histidine; Inclusion Bodies; Isopropyl Thiogalactoside; Molecular Sequence Data; Peptides; Radioimmunoassay; Receptors, Somatotropin; Recombinant Fusion Proteins; Sequence Analysis; Sheep | 1997 |
Overexpression and purification of an immunologically reactive His-BIV capsid fusion protein.
Topics: Animals; Antibodies, Monoclonal; Capsid; Cattle; Chromatography, Affinity; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gene Products, gag; Histidine; Immunodeficiency Virus, Bovine; Isopropyl Thiogalactoside; Open Reading Frames; Polymerase Chain Reaction; Recombinant Fusion Proteins; Sepharose | 1999 |
Ligand-induced conformational changes in lactose repressor: a phosphorescence and ODMR study of single-tryptophan mutants.
Topics: Amino Acid Substitution; Bacterial Proteins; Escherichia coli; Escherichia coli Proteins; Histidine; Isopropyl Thiogalactoside; Lac Repressors; Lactose; Ligands; Luminescent Measurements; Macromolecular Substances; Magnetic Resonance Spectroscopy; Models, Molecular; Mutagenesis, Site-Directed; Phenylalanine; Protein Conformation; Repressor Proteins; Tryptophan; Tyrosine | 1999 |
Purification of recombinant plasminogen activator inhibitor-1 in the active conformation by refolding from inclusion bodies.
Topics: Cell Fractionation; Chromatography, Affinity; Chromatography, Gel; Cloning, Molecular; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Female; Gene Expression; Genetic Vectors; Guanidine; Histidine; Humans; Immunoblotting; Inclusion Bodies; Isopropyl Thiogalactoside; Oligopeptides; Placenta; Plasminogen Activator Inhibitor 1; Plasminogen Activators; Pregnancy; Protein Conformation; Protein Folding; Recombinant Proteins; Spectrophotometry; Temperature | 2003 |
Bacterial expression, purification, and characterization of rat kidney-type mitochondrial glutaminase.
Topics: Animals; Blotting, Western; Chromatography, Affinity; Chromatography, Gel; Cloning, Molecular; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Escherichia coli; Exons; Gene Deletion; Gene Expression; Genetic Vectors; Glutamate Dehydrogenase; Glutaminase; Glutamine; Histidine; Isopropyl Thiogalactoside; Kinetics; Mitochondria; Oligopeptides; Phosphates; Polymerase Chain Reaction; Protein Binding; Protein Structure, Quaternary; Rats; Recombinant Proteins; Sonication | 2003 |
Optimised expression and purification of recombinant human indoleamine 2,3-dioxygenase.
Topics: Aminolevulinic Acid; Biochemistry; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Heme; Histidine; Humans; Indoleamine-Pyrrole 2,3,-Dioxygenase; Iron; Isopropyl Thiogalactoside; Kynurenine; Plasmids; Protoporphyrins; Recombinant Proteins; Temperature; Time Factors; Tryptophan; Tryptophan Oxygenase | 2004 |
Expression, refolding, purification, and bioactivity of recombinant bifunctional protein, hIL-2/GM-CSF.
Topics: Blotting, Western; Cell Proliferation; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Endotoxins; Escherichia coli; Genetic Vectors; Granulocyte-Macrophage Colony-Stimulating Factor; Histidine; Inclusion Bodies; Interleukin-2; Isopropyl Thiogalactoside; Limulus Test; Plasmids; Protein Renaturation; Recombinant Fusion Proteins | 2005 |
An improved strategy for high-level production of TEV protease in Escherichia coli and its purification and characterization.
Topics: Chaperonin 10; Chaperonin 60; Chromatography, Affinity; Cloning, Molecular; Endopeptidases; Enzyme Induction; Escherichia coli; Escherichia coli Proteins; Histidine; Isopropyl Thiogalactoside; Molecular Chaperones; Oligopeptides; Peptidylprolyl Isomerase; Recombinant Fusion Proteins; Solubility; Temperature | 2007 |
Affinity purification of plasmid DNA directly from crude bacterial cell lysates.
Topics: Aspartic Acid; Bacteria; Bacterial Proteins; Chromatography, Affinity; DNA, Superhelical; Escherichia coli; Escherichia coli Proteins; Freeze Drying; Green Fluorescent Proteins; Histidine; Isopropyl Thiogalactoside; Lac Repressors; Plasmids; Recombinant Fusion Proteins; Repressor Proteins; Reproducibility of Results; Ribonuclease, Pancreatic; Sepharose | 2007 |
Functional expression and purification of bovine enterokinase light chain in recombinant Escherichia coli.
Topics: Amino Acid Sequence; Chromatography, Affinity; Cloning, Molecular; Enteropeptidase; Escherichia coli; Gene Expression; Genetic Vectors; Histidine; Isopropyl Thiogalactoside; Oligopeptides; Periplasm; Protein Engineering; Protein Folding; Recombinant Fusion Proteins; Recombinant Proteins; Substrate Specificity; Transformation, Bacterial | 2007 |
Four disulfide-bridged scorpion beta neurotoxin CssII: heterologous expression and proper folding in vitro.
Topics: Animals; Cell Line; Circular Dichroism; Cloning, Molecular; Disulfides; Dose-Response Relationship, Drug; Escherichia coli; Genes, Synthetic; Histidine; Humans; In Vitro Techniques; Inclusion Bodies; Injections, Intraperitoneal; Isopropyl Thiogalactoside; Lethal Dose 50; Male; Mice; Mice, Inbred Strains; Molecular Weight; Neurotoxins; Oxidation-Reduction; Patch-Clamp Techniques; Plasmids; Protein Folding; Recombinant Proteins; Scorpion Venoms; Sodium Channels; Transfection | 2007 |
Effective high-throughput overproduction of membrane proteins in Escherichia coli.
Topics: Cloning, Molecular; Escherichia coli; Histidine; Isopropyl Thiogalactoside; Legionella pneumophila; Membrane Proteins; Phosphorylation; Protein Engineering; Recombinant Fusion Proteins; Temperature | 2008 |
Separation and purification of Escherichia coli-expressed human thymosin-α1 using affinity chromatography and high-performance liquid chromatography.
Topics: Chromatography, Affinity; Chromatography, High Pressure Liquid; Cloning, Molecular; Escherichia coli; Gene Expression; Histidine; Humans; Isoelectric Focusing; Isopropyl Thiogalactoside; Oligopeptides; Protein Denaturation; Recombinant Fusion Proteins; Reproducibility of Results; Solubility; Thymalfasin; Thymosin | 2011 |
Crystallization and preliminary X-ray crystallographic studies of β-transaminase from Mesorhizobium sp. strain LUK.
Topics: Alphaproteobacteria; Amino Acid Sequence; Amino Acids; Bacterial Proteins; Crystallization; Crystallography, X-Ray; Diffusion; Escherichia coli; Histidine; Intramolecular Transferases; Isopropyl Thiogalactoside; Molecular Sequence Data; Molecular Weight; Protein Engineering; Sequence Homology, Amino Acid; Stereoisomerism; Substrate Specificity; Transaminases; Transformation, Bacterial; X-Ray Diffraction | 2011 |
Improvement of the expression and purification of Mycobacterium tuberculosis arylamine N-acetyltransferase (TBNAT) a potential target for novel anti-tubercular agents.
Topics: Arylamine N-Acetyltransferase; Bacterial Proteins; Buffers; Chromatography, Gel; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Enzyme Assays; Escherichia coli; Histidine; Isopropyl Thiogalactoside; Molecular Weight; Mycobacterium tuberculosis; Plasmids; Protein Stability; Recombinant Proteins; Solubility; Spectrometry, Mass, Electrospray Ionization; Temperature | 2011 |
Heterologous expression, chaperone mediated solubilization and purification of parasitic nematode-specific growth factor-like protein of Setaria digitata.
Topics: Animals; Cloning, Molecular; Culture Media; Escherichia coli; Helminth Proteins; Histidine; Isopropyl Thiogalactoside; Molecular Chaperones; Oligopeptides; Recombinant Proteins; Setaria Nematode | 2014 |
Skimmed milk as an alternative for IPTG in induction of recombinant protein expression.
Topics: Animals; Bacterial Proteins; Cloning, Molecular; Escherichia coli; Flagellin; Gene Expression; Genetic Vectors; Histidine; Isopropyl Thiogalactoside; Lac Operon; Lactose; Milk; Oligopeptides; Recombinant Fusion Proteins; Salmonella typhimurium | 2020 |
Temoneira-1 β-lactamase is not a metalloenzyme, but its native metal ion binding sites allow for purification by immobilized metal ion affinity chromatography.
Topics: Anti-Bacterial Agents; beta-Lactamases; beta-Lactams; Binding Sites; Chromatography, Affinity; Histidine; Imidazoles; Ions; Isopropyl Thiogalactoside; Metalloproteins; Penicillinase; Protein Sorting Signals; Serine | 2023 |