hispidulin and cirsimaritin

Topics: Animals; Anti-Inflammatory Agents; Baccharis; Disease Models, Animal; Ear Diseases; Edema; Flavones; Male; Mice; Mice, Inbred ICR; Terpenes; Tetradecanoylphorbol Acetate

A reversed-phase HPLC procedure is proposed for the determination of seven flavonoids (luteolin, nepetin, hispidulin, jaceosidin, cirsimaritin, cirsilineol, and eupatorin) in vervain samples. A simple extractive technique was developed, involving only extraction with ethyl ether, which allowed the elimination of phenolic acids, yielding a sufficiently purified flavonoid fraction. All of the analyzed vervain samples showed a common flavonoid pattern, in which hispidulin and jaceosidin were the major compounds and nepetin was the minor compound.

Topics: Chromatography, High Pressure Liquid; Flavones; Flavonoids; Luteolin; Plant Extracts

Two flavones, 4',5,7-trihydroxy-6-methoxy flavone (dinatin) and 4',5-dihydroxy-6, 7-dimethoxy flavone (skrofulein), were extracted from Artemisia herba alba L. Dinatin and skrofulein inhibited the binding of [methyl-3H]diazepam to rat brain membranes in vitro with IC50 of 1.3 and 23 mumol.L-1, respectively. The GABA-ratios (the ratio of IC50 values in the absence/presence of GABA in the binding assay) were 1.1 and 1.2 for dinatin and skrofulein, respectively. Both flavones induced a slight increase in [35S] TBPS binding. The data suggest that the flavones are antagonists or partial agonists of benzodiazepine receptors.

Topics: Animals; Binding, Competitive; Brain; Cell Membrane; Diazepam; Flavones; Flavonoids; GABA-A Receptor Agonists; GABA-A Receptor Antagonists; Plants, Medicinal; Rats; Rats, Wistar