hispidin and hispolon

Topics: Antigens, CD; Apoptosis; Basidiomycota; Catechols; Cells, Cultured; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Dendritic Cells; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; Humans; Killer Cells, Natural; Plant Extracts; Pyrones; T-Lymphocytes

The inhibitory activity from the isolated component of the fruiting body Phellinus merrillii (PM) was evaluated against α-glucosidase and lens aldose reductase from Sprague-Dawley male rats and compared to the quercetin as an aldose reductase inhibitor and acarbose as an α-glucosidase inhibitor. The ethanol extracts of PM (EPM) showed the strong α-glucosidase and aldose reductase activities. α-Glucosidase and aldose reductase inhibitors were identified as hispidin (A), hispolon (B), and inotilone (C), which were isolated from EtOAc-soluble fractions of EPM. The above structures were elucidated by their spectra and comparison with the literatures. Among them, hispidin, hispolon, and inotilone exhibited potent against α-glucosidase inhibitor activity with IC(50) values of 297.06 ± 2.06, 12.38 ± 0.13, and 18.62 ± 0.23 μg/mL, respectively, and aldose reductase inhibitor activity with IC(50) values of 48.26 ± 2.48, 9.47 ± 0.52, and 15.37 ± 0.32 μg/mL, respectively. These findings demonstrated that PM may be a good source for lead compounds as alternatives for antidiabetic agents currently used. The importance of finding effective antidiabetic therapeutics led us to further investigate natural compounds.

Topics: Aldehyde Reductase; Animals; Basidiomycota; Catechols; Enzyme Inhibitors; Fruiting Bodies, Fungal; Furans; Glycoside Hydrolase Inhibitors; Hypoglycemic Agents; Lens, Crystalline; Macrolides; Male; Pyrones; Rats; Rats, Sprague-Dawley

The antiviral activity of two phenolic compounds, hispolon and hispidin, isolated from the fruit bodies of the basidiomycete Inonotus hispidus as well as of some extracts prepared from fruit bodies and mycelial cultures of this fungus was investigated in allantois on the shell-test system. Ethanolic extracts, hispidin and hispolon showed considerable antiviral activity against influenza viruses type A and B.

Topics: Antiviral Agents; Basidiomycota; Catechols; Dose-Response Relationship, Drug; Fruit; Humans; Influenza A virus; Influenza B virus; Microbial Sensitivity Tests; Phytotherapy; Plant Extracts; Pyrones

The effects of two phenolic compounds, hispolon and hispidin isolated from fruit bodies of the basidiomycete Inonotus hispidus (Bull. ex Fr.) Karst, were investigated on the chemiluminescence response by LPS- or zymosan-activated human mononuclear cells (MNC) and on the concanavalin A-induced proliferation of spleen lymphocytes of mouse in vitro. Both compounds showed inhibitory activity in the chemiluminescence-test with an IC50 (the concentration of test compound causing 50% effect) ranging from 4.4 to 4.6 micrograms/ml (20.3 to 21.2 microM) for hispolon and < 0.1 to 1.5 micrograms/ml (from < 0.4 microM to 6.0 microM) for hispidin. Antiproliferative effects have been achieved in the lymphocyte transformation test (LTT) by hispolon with an IC50 of 3.4 micrograms/ml (15.5 microM).

Topics: Animals; Basidiomycota; Catechols; Cell Division; Cells, Cultured; Concanavalin A; Humans; Lipopolysaccharides; Luminescent Measurements; Lymphocytes; Mice; Mice, Inbred ICR; Mitogens; Monocytes; Pyrones; Spleen; Thymidine; Zymosan