hexacyanoferrate-iii and aniline

Using a small-molecule-based screen, ferricyanide was identified as a mild and efficient oxidant for the coupling of anilines and o-aminophenols on protein substrates. This reaction is compatible with thiols and 1,2-diols, allowing its use in the creation of complex bioconjugates for use in biotechnology and materials applications.

Topics: Aminophenols; Aniline Compounds; Ferricyanides; Models, Molecular; Molecular Structure; Oxidation-Reduction

β-galactosidase splits lactose into glucose and galactose. Because of its biotechnological interest, we presented a biosensor system in order to monitor β-galactosidase activity. Immobilization steps of the biosensor were identified by cyclic voltammograms and electrochemical impedance spectroscopy. β-galactosidase was voltammetrically detected at about +150 mV (vs. Ag/AgCl) in citrate buffer solution (0.05 M, pH 4.8). The linear response for β-galactosidase detection was in the range of 0.0118 U mL(-1)to 0.47 U mL(-1)and a shorter response time of ∼50 s. Our results demonstrated the biosensor's electrochemical properties and analytical characteristics were very useful and effective for monitoring of β-galactosidase activity.

Topics: Aniline Compounds; beta-Galactosidase; Biosensing Techniques; Chemistry Techniques, Analytical; Electrochemistry; Electrodes; Electron Transport; Enzymes, Immobilized; Ferricyanides; Galactose; Glass; Glucose; Glucose Oxidase; Lactose; Polymerization; Sensitivity and Specificity

A synthetic flavocytochrome with the reductase and oxygenase activities was obtained by covalent binding of riboflavin to cytochrome P450 2B4. The reactions catalyzed by the newly synthesized flavocytochromes were studied. Formation of carboxycomplex with the reduced form of hemoprotein led to 60-80% inhibition of oxygenase reactions indicating the leading role of reduced heme iron in generating active oxygen species by flavocytochromes.

Topics: Aniline Compounds; Animals; Aryl Hydrocarbon Hydroxylases; Cytochrome c Group; Cytochrome P-450 Enzyme System; Ferricyanides; Hydroxylation; Male; Methylation; NAD; NADP; Oxidoreductases; Oxygenases; Rabbits; Riboflavin; Spectrophotometry; Steroid Hydroxylases