hexabromodiphenyl-ether-154 and decabromobiphenyl-ether

This study evaluated decabromodiphenyl ether (BDE-209) anaerobic debromination and bacterial community changes in mangrove sediment. BDE-209 debromination rates were enhanced with zerovalent iron compared to without zerovalent iron in the sediment. BDE-209 debromination rates in microcosms constructed with sediments collected in autumn were higher than in microcosms constructed with sediments collected in spring and were higher at the Bali sampling site than the Guandu sampling site. The intermediate products resulting from the reductive debromination of BDE-209 in sediment were nona-BDE (BDE-206, BDE-207), octa-BDEs (BDE-196, BDE-197), hepta-BDEs (BDE-183, BDE-184, BDE-191), hexa-BDEs (BDE-137, BDE-138, BDE-154, BDE-157), penta-BDEs (BDE-85, BDE-99, BDE-100, BDE-126), tetra-BDEs (BDE-47, BDE-49, BDE-66, BDE-77), tri-BDEs (BDE-17, BDE-28), and di-BDEs (BDE-15). Fifty bacterial genera associated with BDE-209 debromination were identified. Overall, 12 of the 50 bacterial genera were reported to be involved in dehalogenation of aromatic compounds. These bacteria have high potential to be BDE-209 debromination bacteria. Different combinations of bacterial community composition exhibit different abilities for BDE-209 anaerobic debromination.

Topics: Bacteria; Biodegradation, Environmental; Halogenated Diphenyl Ethers; Polybrominated Biphenyls; Wetlands

Indiscriminate use of synthetic substances has led to environmental contamination and increasing human and animal exposure to harmful chemicals. Polybrominated flame retardants (PBDEs), which serve as non-covalent additives that enhance the safety of a variety of commercial and consumer goods, are an important class among potentially damaging synthetic substances. Its use is very common in developing countries, including Brazil. In theory, 209 different PBDE congeners exist, and many are currently being used during the manufacture of several products. Unfortunately, PBDEs are easily released from the original products, promptly reaching the environment. Knowledge about the toxicological power of these substances is still limited, which has prevented environmental and regulatory authorities from conducting adequate risk assessments. This research addresses the genotoxic and mutagenic potential of PBDEs. The effects of HepG2 cells and Salmonella typhimurium exposure to six main representatives of PBDEs, namely tetrabromodiphenyl ether (BDE-47), pentabromodiphenyl ether (BDE-99 and BDE-100), hexabromodiphenyl ether (BDE-153 and BDE-154) and decabromodiphenyl ether (BDE-209), were evaluated. The comet assay revealed that all the assessed BDEs exerted genotoxic effects but induced no micronuclei formation in HepG2 cells. These BDEs had no significant mutagenic effects on the Salmonella typhimurium strains TA98 and TA100. Taken together, the results of the genomic instability assays showed that PBDEs can represent a risk to the health of directly and indirectly exposed population, because the assessed BDEs induce genotoxic effects in the HepG2 cell line.

Topics: Comet Assay; Environmental Pollutants; Flame Retardants; Halogenated Diphenyl Ethers; Hep G2 Cells; Humans; Isomerism; Micronucleus Tests; Molecular Weight; Mutagenicity Tests; Mutagens; Osmolar Concentration; Polybrominated Biphenyls; Salmonella typhimurium; Solubility; Transition Temperature

Accumulation and evidence of debromination of the flame retardant 2,2',3,3',4,4',5,5',6,6'-decabromodiphenyl ether (BDE-209) have been reported for biota, including raptorial birds, based on PBDE congener residues in tissues and eggs. However, in vivo studies with BDE-209-exposed birds are rare and unknown for a raptorial species. In the present study, males (n=22) of raptorial American kestrels (Falco sparverius) were exposed to 116,000ng of BDE-209 (high purity, >98%; in safflower oil) per day for 21days (~2,436,000ng total BDE-209 exposure over this uptake period), followed by a 25-day depuration period. Control males (n=11) received the safflower vehicle only. In the exposed birds, BDE-209 was quantifiable in all plasma (end of uptake and depuration period) as well as liver and fat (end of depuration only) samples. The mean (±SE) BDE-209 level in plasma was 1474±1145ng/g wet weight (ww) at the end of the uptake period, and was significantly (p<0.001) lower (88%) at 174±148ng/g ww after the 25day depuration period. This equates to a mean reduction rate of 52ng/g ww per day and a rough estimation of the BDE-209 half-life in plasma of approximately 14days. The mean (±SE) BDE-209 levels were 4668±6192ng/g ww in the fat, and 338±311ng/g ww in the liver, of exposed individuals, which were significantly (p≤0.001) greater than mean concentrations (25±20 in fat and 2.6±0.9ng/g ww in liver) in the control birds. In addition to BDE-209, lower brominated PBDE congeners, and mainly meta- and para-debromination products of BDE-209 were also quantified in plasma, liver and/or fat. We estimated based on the dose that at least 80% of the non-BDE-209 concentration in the kestrel tissues and plasma must be derived from BDE-209 debromination by the kestrels. Where quantifiable, lower brominated PBDE concentrations were significantly (0.023>p>0.001) higher in the exposed relative to the control bird samples (except for BDE-154 and -153 in fat). Additional PBDE congeners found in plasma included nona-BDEs (208, 207 and 206), followed by octa-BDEs (197, 196, 201 and 203), and in liver and/or fat, the hepta-BDEs 180 and 183 and BDE-153. Higher hepatic EROD activity (cytochrome P450 1A1 monooxygenase-mediation) in the exposed birds compared to control birds was strongly suggested to be PBDE-induced, and was consistent with BDE-209 and congener metabolism in the exposed kestrels. The mean EROD activity rate was 36.1pmol/min/mg protein relative to the (n=4) control birds whose

Topics: Animals; Cytochrome P-450 Enzyme System; Diet; Environmental Exposure; Enzyme Induction; Falconiformes; Flame Retardants; Half-Life; Halogenated Diphenyl Ethers; Liver; Male; Polybrominated Biphenyls; United States

Polybrominated diphenyl ethers (PBDEs) used extensively over the past 3 decades as flame retardants in most types of polymers, all over the world, have been identified as global pollutants. PBDEs pose various health problems such as thyroid hormone disruption, permanent learning and memory impairment, behavioral changes, hearing deficits, delayed puberty onset, fetal malformations, and possibly cancer. Many measurements of PBDEs in various matrices from Sweden, Holland, Japan, the USA, and elsewhere have been reported, but few measurements are available for India. In this study, a preliminary screening of different congeners of PBDEs has been performed in different old electronic and consumer products with an objective to build capacity in order to analyze PBDEs and BFRs. Six different samples, foam from upholstery, motherboard of a computer, children toy composite sample, old vanishing window blind sample, electrical wire sample, and PVC flooring sample, were collected and analyzed for the presence of the following PBDE congeners: BDE-28, BDE-47, BDE-99, BDE-100, BDE-153, BDE-154, BDE-183, and BDE-209. It was found that three out of six samples were positive for the presence of PBDEs. Three congeners were detected in the samples, i.e., BDE-47, BDE-153, and BDE-209, of which, highest concentration was of BDE-209. Among the samples, motherboard of computer showed the highest concentration of BDE-209 followed by window blind and foam from upholstery. The results of this preliminary investigation indicate that PBDEs are still present in the old consumer products which can be an important additional source of exposure to the population.

Topics: Environmental Monitoring; Flame Retardants; Halogenated Diphenyl Ethers; Hazardous Substances; India; Manufactured Materials; Play and Playthings; Polybrominated Biphenyls

Twenty-eight sediment samples were collected from the Taihu Lake and analyzed to acquire information about the levels, distribution, possible sources, time trend and inventory of polybrominated diphenyl ethers (PBDEs) in the Taihu Lake. Our results showed that the most abundant BDE congeners in surface sediments were BDE-47, 99 and 209, with a median value of 0.124, 0.279, and 22.72 ng(gdw)(-1), respectively. The levels of BDE-209 in our samples were much higher relative to those of the other BDE congeners and made up more than 80% of the PBDEs levels in almost all samples. Disregarding BDE-209, among the seven PBDEs (BDE-28, 47, 99, 100, 153, 154, 183) more concerned, the most abundant ones were BDE-47 and 99, which contributed 44.65% (ranged from 29.02% to 73.11%), 24.24% (ranged from 2.27% to 39.66%) to ∑(7)PBDEs, respectively. The correlation between PBDE and TOC was poor, indicating that PBDEs concentrations in sediments of the Taihu Lake were controlled not only by TOC contents, but also by a combined effect of transport, mixing, depositional mechanisms associated with PBDEs, uncontaminated sediments, or fresh input of PBDEs. As expected, the time trend of PBDEs concentration in surface sediment from Taihu Lake were increasing, and the inventory of ∑(25)PBDEs and BDE-209 were estimated to be 3668 kg and 26296 kg.

Topics: China; Environmental Monitoring; Geologic Sediments; Halogenated Diphenyl Ethers; Lakes; Polybrominated Biphenyls

Human serum and mother's milk are frequently used to assess exposure to polybrominated diphenyl ethers (PBDEs), including transplacental transfer to the foetus. However, little is known about the kinetics of PBDEs, especially the highly brominated BDE congeners. In this pilot study, maternal serum samples were collected from 10 women at delivery and five to six weeks post partum. Umbilical serum was also obtained. Milk was donated two to five days, and five to six weeks after delivery. The amount of PBDEs in these samples was determined using liquid-liquid extraction and GC/MS. Low, moderately and highly brominated diphenyl ethers were present in umbilical cord serum, indicating placental transfer. The lipid-adjusted levels of BDE-47, BDE-207 and BDE-209 were similar in maternal and umbilical cord serum, whereas the cord serum levels for the penta- to octa-BDEs quantified were lower than in maternal serum. Marked changes were seen in the congener pattern in breast milk during the first month of lactation, whereas maternal serum levels did not change significantly. The general pattern was an enrichment of low to moderately brominated congeners (i.e. from BDE-17 to BDE-154, with the exception of BDE-28) in colostrum compared with maternal serum. In contrast, more highly brominated congeners were found at similar, or lower levels in colostrum than in maternal serum. After the transition from colostrum to mature milk, the levels of BDE-153 and BDE-209 were substantially reduced, and BDE-209 was below the limit of detection in 6 out of 9 samples. A literature review on the design and reporting of studies on the transfer of PBDEs from mother to infant revealed a lack of transparency in many cases. The use of the recently published STROBE-ME guidelines is therefore recommended.

Topics: Adult; Colostrum; Environmental Pollutants; Environmental Pollution; Female; Fetal Blood; Halogenated Diphenyl Ethers; Humans; Infant, Newborn; Maternal Exposure; Milk, Human; Pilot Projects; Polybrominated Biphenyls; Pregnancy; Young Adult

Polybrominated diphenyl ether (PBDE) flame retardants are known to affect thyroid hormone (TH) regulation. The TH-regulating deiodinases have been implicated in these impacts; however, PBDE effects on the fish thyroid system are largely unknown. Moreover, the liver as a potential target of PBDE toxicity has not been explored in young fish. This study measured decabromodiphenyl ether (BDE-209) effects on TH regulation by measuring deiodinase activity in juvenile fathead minnows (Pimephales promelas). Dietary accumulations and debromination of BDE-209 were also measured, and the morphology of thyroid and liver tissues was examined. Juvenile fathead minnows (28 days old) received a 28-day dietary treatment of BDE-209 at 9.8 ± 0.16 μg/g of food at 5% of their body weight per day followed by a 14-day depuration period in which they were fed clean food. Chemical analysis revealed that BDE-209 accumulated in tissues and was metabolized to reductive products ranging from penta- to octaBDEs with 2,2',4,4',5,6'-hexabromodiphenyl ether (BDE-154) being the most accumulative metabolite. By day 28 of the exposure, rates of outer and inner ring deiodination (ORD and IRD, respectively) of thyroxine (T4) were each reduced by ∼74% among treatments. Effects on T4-ORD and T4-IRD remained significant even after the 14-day depuration period. Histological examination of treated fish showed significantly increased thyroid follicular epithelial cell heights and vacuolated hepatocyte nuclei. Enlarged biliary passageways may be the cause of the distinctive liver phenotype observed, although further testing is needed. Altogether, these results suggest that juvenile fish may be uniquely susceptible to thyroid disruptors like PBDEs.

Topics: Animals; Cyprinidae; Diet; Endocrine Disruptors; Environmental Exposure; Flame Retardants; Glutathione Transferase; Halogenated Diphenyl Ethers; Iodide Peroxidase; Liver; Thyroid Gland; Thyroxine