heparitin-sulfate and monodansylcadaverine

Fish sperm head plasma membranes have been demonstrated to contain syndecan (transmembrane heparan sulfate proteoglycan) immunofluorohistochemically and using immunoblot analysis and transglutaminase (TGase) by histochemistry. In order to examine the involvement of syndecan in fertilization, mature eggs were inseminated by direct mixing with untreated sperm or with sperm pretreated with an antiheparan sulfate (HS) antibody monoclonal (mAb), bovine serum albumin, human transferrin, or a TGase inhibitor (monodansylcadaverine, cystamine, and iodoacetamide). The fertilization rates of eggs inseminated with untreated and albumin-pretreated sperm were approximately 99.3% and 94.7%, respectively. Those of eggs pretreated with the anti-HS antibody and transferrin were 0% and 5.4%, respectively, whereas use of sperm pretreated with TGase inhibitors resulted in fertilization rates of approximately 13.2-17.8%. These results indicate that sperm head syndecan play an important role in fish sperm-egg contact and/or binding and that TGase inhibitors may reduce the fertilization rate by inhibiting sperm motility.

Topics: Animals; Antibodies, Monoclonal; Cadaverine; Carps; Cattle; Cell Membrane; Cystamine; Enzyme Inhibitors; Female; Fertilization; Fishes; Heparitin Sulfate; Humans; Iodoacetamide; Male; Membrane Glycoproteins; Microscopy, Electron, Scanning; Oncorhynchus mykiss; Ovum; Proteoglycans; Serum Albumin, Bovine; Sperm Head; Sperm-Ovum Interactions; Spermatozoa; Syndecans; Transferrin; Transglutaminases