heparitin-sulfate and anthranilamide

A series of disaccharides derived from chondroitin sulfate and heparin/heparan sulfate were derivatized at their reducing ends with a fluorophore 2-aminobenzamide to develop a sensitive microanalytical method for glycosaminoglycans. The resulting labeled compounds derived from chondroitin sulfate or heparin/heparan sulfate were well-separated and quantified by HPLC equipped with a fluorescence detector. The detection limit was a low picomole level. This method was applied to the analysis of the disaccharide composition of tetra- and hexasaccharides derived from chondroitin sulfate and heparin/heparan sulfate as well as these glycosaminoglycan polysaccharides. The method was also successfully applied to the exosequencing of chondrohexasaccharides, where the fluorophore-labeled oligosaccharides were degraded exolytically from the nonreducing ends using bacterial eliminases. The resultant labeled fragments were identified by HPLC.

Topics: Carbohydrate Sequence; Chondroitin Sulfates; Chromatography, High Pressure Liquid; Disaccharides; Fluorescent Dyes; Glycosaminoglycans; Heparin; Heparitin Sulfate; Molecular Sequence Data; Oligosaccharides; ortho-Aminobenzoates; Sensitivity and Specificity