harmine and 4-aminophenol

In human lymphocytes, aniline was unable to induce an increase of SCEs in vitro with or without metabolic activation by S9 mix. p-Aminophenol, one of the C-hydroxylation metabolites of aniline in the body, however, increased the SCE frequencies of lymphocytes at a concentration of 10-4 M. The addition of norharman to aniline plus S9 mix increased the SCE frequencies. The increase, however, was due to the SCE-inducing activity of norharman. These data show that the addition of norharman, which enhances the sensitivity of the Salmonella/microsome test, does not produce an enhancement of the sensitivity of the SCE test.

Topics: Alkaloids; Aminophenols; Aniline Compounds; Animals; Biotransformation; Carbolines; Crossing Over, Genetic; Harmine; Humans; Lymphocytes; Male; Microsomes, Liver; Rats; Sister Chromatid Exchange