harman and propyl-beta-carboline-3-carboxylate

The beta-carbolines harmane and norharmane competitively inhibit [3H]flunitrazepam ([3H]FNZ) binding to deoxycholate-solubilized benzodiazepine receptors from calf cerebral cortex, with Ki in the micromolar range [3H]Propyl-beta-carboline-3-carboxylate ([3H]PrCC) binds to the soluble receptors with an affinity similar to its binding to particulate receptors (0.41 nM vs 0.48 nM, respectively). The component that binds [3H]PrCC displays a sedimentation profile on sucrose gradient centrifugation similar to that of [3H]FNZ binding component (sedimentation coefficient about 11S).

Topics: Alkaloids; Animals; Benzodiazepines; Carbolines; Cattle; Cerebral Cortex; Deoxycholic Acid; Flunitrazepam; Harmine; Receptors, Drug; Solubility; Statistics as Topic; Tritium