harman has been researched along with 1-methyl-4-(2--methylphenyl)-1-2-3-6-tetrahydropyridine* in 1 studies
1 other study(ies) available for harman and 1-methyl-4-(2--methylphenyl)-1-2-3-6-tetrahydropyridine
| Article | Year |
|---|---|
1-Methyl-4-phenylpyridinium (MPP+) binds with high affinity to a beta-carboline binding site located on monoamine oxidase type A in rat brain.
The in vitro binding of [3H]pargyline and [3H]harman ([3H]1-methyl-beta-carboline) to monoamine oxidase A (MAO-A; EC 1.4.3.4) on membranes of rat cerebral cortex was evaluated. Displacement of the [3H]pargyline binding on MAO-A (L(-)-deprenyl suppressed binding to MAO-B) by harman, 1-methyl-4-phenylpyridinium (MPP+) and 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) revealed IC50 values of 250 +/- 100 nM, 3.1 +/- 0.8 microM, and 5.1 +/- 0.4 microM, respectively. Displacement of the selective, reversible, high-affinity [3H]harman binding to MAO-A revealed inhibition in a competitive manner with Hill coefficients about unity of each compound tested and calculated apparent Ki-values of 4.7 +/- 2.0 nM, 91 +/- 13 nM and 2.4 +/- 0.8 microM, respectively. The data of [3H]harman displacement support the hypothesis of a high-affinity binding site of the neurotoxin MPP+ located on mitochondrial MAO-A with a significant influence on the development of MPTP induced parkinsonism. Topics: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; Animals; Binding, Competitive; Brain; Carbolines; Cerebral Cortex; Harmine; Male; Monoamine Oxidase; Pargyline; Pyridinium Compounds; Rats; Rats, Wistar | 1993 |