guanosine-triphosphate and ribose-5-phosphate

guanosine-triphosphate has been researched along with ribose-5-phosphate* in 1 studies

Other Studies

1 other study(ies) available for guanosine-triphosphate and ribose-5-phosphate

Article	Year
ATP-binding properties of P glycoprotein from multidrug-resistant KB cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 1987, Volume: 1, Issue:1 The photoaffinity reagent 8-azido-alpha-[32P]ATP was used to label a protein of 170 kDa in membrane vesicle preparations from a highly multidrug-resistant cell line, KB-V1, but not from the drug-sensitive parental cell line KB-3-1. The 170-kDa labeled protein was immunoprecipitated with a monoclonal antibody (MRK-16) to P glycoprotein. Both ATP and GTP inhibited labeling by 8-azido-alpha-[32P]ATP. Labeling of P170 was not inhibited by 5 mM ADP, 5 mM ribose-5-phosphate, or 100 microM vinblastine. These data directly demonstrate that P glycoprotein has a nucleotide-binding site that could supply energy for drug transport. Topics: Adenosine Diphosphate; Adenosine Triphosphate; Affinity Labels; ATP Binding Cassette Transporter, Subfamily B, Member 1; Azides; Cell Membrane; Drug Resistance; Glycoproteins; Guanosine Triphosphate; Humans; KB Cells; Molecular Weight; Photochemistry; Protein Binding; Ribosemonophosphates	1987

Article

Year

ATP-binding properties of P glycoprotein from multidrug-resistant KB cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 1987, Volume: 1, Issue:1

The photoaffinity reagent 8-azido-alpha-[32P]ATP was used to label a protein of 170 kDa in membrane vesicle preparations from a highly multidrug-resistant cell line, KB-V1, but not from the drug-sensitive parental cell line KB-3-1. The 170-kDa labeled protein was immunoprecipitated with a monoclonal antibody (MRK-16) to P glycoprotein. Both ATP and GTP inhibited labeling by 8-azido-alpha-[32P]ATP. Labeling of P170 was not inhibited by 5 mM ADP, 5 mM ribose-5-phosphate, or 100 microM vinblastine. These data directly demonstrate that P glycoprotein has a nucleotide-binding site that could supply energy for drug transport.

Topics: Adenosine Diphosphate; Adenosine Triphosphate; Affinity Labels; ATP Binding Cassette Transporter, Subfamily B, Member 1; Azides; Cell Membrane; Drug Resistance; Glycoproteins; Guanosine Triphosphate; Humans; KB Cells; Molecular Weight; Photochemistry; Protein Binding; Ribosemonophosphates

1987