guanosine-triphosphate and phosphatidylinositol-5-phosphate

guanosine-triphosphate has been researched along with phosphatidylinositol-5-phosphate* in 2 studies

Other Studies

2 other study(ies) available for guanosine-triphosphate and phosphatidylinositol-5-phosphate

Article	Year
The Lipid Kinase PI5P4Kβ Is an Intracellular GTP Sensor for Metabolism and Tumorigenesis. Molecular cell, 2016, Jan-21, Volume: 61, Issue:2 While cellular GTP concentration dramatically changes in response to an organism's cellular status, whether it serves as a metabolic cue for biological signaling remains elusive due to the lack of molecular identification of GTP sensors. Here we report that PI5P4Kβ, a phosphoinositide kinase that regulates PI(5)P levels, detects GTP concentration and converts them into lipid second messenger signaling. Biochemical analyses show that PI5P4Kβ preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation, and its activity reflects changes in direct proportion to the physiological GTP concentration. Structural and biological analyses reveal that the GTP-sensing activity of PI5P4Kβ is critical for metabolic adaptation and tumorigenesis. These results demonstrate that PI5P4Kβ is the missing GTP sensor and that GTP concentration functions as a metabolic cue via PI5P4Kβ. The critical role of the GTP-sensing activity of PI5P4Kβ in cancer signifies this lipid kinase as a cancer therapeutic target. Topics: Adenosine Triphosphate; Amino Acid Sequence; Animals; Carcinogenesis; Cell Proliferation; Crystallography, X-Ray; Guanosine Triphosphate; HEK293 Cells; Humans; Hydrolysis; Intracellular Space; Kinetics; Mice; Molecular Sequence Data; Mutant Proteins; Phosphatidylinositol Phosphates; Phosphotransferases (Alcohol Group Acceptor); Protein Binding; Proteomics; Signal Transduction	2016
PIP4K2B: Coupling GTP Sensing to PtdIns5P Levels to Regulate Tumorigenesis. Trends in biochemical sciences, 2016, Volume: 41, Issue:6 Although guanine nucleotides are essential for cell growth, how their levels are sensed in mammalian cells is unknown. Sumita et al. show that PIP4K2B, a phosphoinositide kinase, is a molecular sensor that transduces changes in GTP into changes in the levels of the phosphoinositide PtdIns5P to modulate tumour cell growth. Topics: Adenosine Triphosphate; Animals; Binding Sites; Cell Line, Transformed; Cell Proliferation; Cell Transformation, Neoplastic; Fibroblasts; Gene Expression Regulation, Neoplastic; Guanosine Triphosphate; Humans; Isoenzymes; Kinetics; Mice; Mice, Nude; Mutation; Phosphatidylinositol Phosphates; Phosphotransferases (Alcohol Group Acceptor); Protein Binding; Signal Transduction	2016

Article

Year

The Lipid Kinase PI5P4Kβ Is an Intracellular GTP Sensor for Metabolism and Tumorigenesis.

Molecular cell, 2016, Jan-21, Volume: 61, Issue:2

While cellular GTP concentration dramatically changes in response to an organism's cellular status, whether it serves as a metabolic cue for biological signaling remains elusive due to the lack of molecular identification of GTP sensors. Here we report that PI5P4Kβ, a phosphoinositide kinase that regulates PI(5)P levels, detects GTP concentration and converts them into lipid second messenger signaling. Biochemical analyses show that PI5P4Kβ preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation, and its activity reflects changes in direct proportion to the physiological GTP concentration. Structural and biological analyses reveal that the GTP-sensing activity of PI5P4Kβ is critical for metabolic adaptation and tumorigenesis. These results demonstrate that PI5P4Kβ is the missing GTP sensor and that GTP concentration functions as a metabolic cue via PI5P4Kβ. The critical role of the GTP-sensing activity of PI5P4Kβ in cancer signifies this lipid kinase as a cancer therapeutic target.

Topics: Adenosine Triphosphate; Amino Acid Sequence; Animals; Carcinogenesis; Cell Proliferation; Crystallography, X-Ray; Guanosine Triphosphate; HEK293 Cells; Humans; Hydrolysis; Intracellular Space; Kinetics; Mice; Molecular Sequence Data; Mutant Proteins; Phosphatidylinositol Phosphates; Phosphotransferases (Alcohol Group Acceptor); Protein Binding; Proteomics; Signal Transduction

2016

PIP4K2B: Coupling GTP Sensing to PtdIns5P Levels to Regulate Tumorigenesis.

Trends in biochemical sciences, 2016, Volume: 41, Issue:6

Although guanine nucleotides are essential for cell growth, how their levels are sensed in mammalian cells is unknown. Sumita et al. show that PIP4K2B, a phosphoinositide kinase, is a molecular sensor that transduces changes in GTP into changes in the levels of the phosphoinositide PtdIns5P to modulate tumour cell growth.

Topics: Adenosine Triphosphate; Animals; Binding Sites; Cell Line, Transformed; Cell Proliferation; Cell Transformation, Neoplastic; Fibroblasts; Gene Expression Regulation, Neoplastic; Guanosine Triphosphate; Humans; Isoenzymes; Kinetics; Mice; Mice, Nude; Mutation; Phosphatidylinositol Phosphates; Phosphotransferases (Alcohol Group Acceptor); Protein Binding; Signal Transduction

2016