guanosine-triphosphate and nile-red

guanosine-triphosphate has been researched along with nile-red* in 1 studies

Other Studies

1 other study(ies) available for guanosine-triphosphate and nile-red

Article	Year
Synthesis of 2'-O-propargyl nucleoside triphosphates for enzymatic oligonucleotide preparation and "click" modification of DNA with Nile red as fluorescent probe. Bioconjugate chemistry, 2013, Mar-20, Volume: 24, Issue:3 Uridine, adenosine, guanosine, and cytidine that carry a propargyl group attached to the 2'-oxygen were converted efficiently to the corresponding nucleoside triphosphates (pNTPs). Primer extension experiments revealed that pUTP, pATP, and pGTP can be successfully incorporated in oligonucleotides in the so-called 9°N and Therminator DNA polymerases. Most importantly, the ethynyl group as single 2'-modification of the enzymatically prepared oligonucleotides can be applied for postsynthetic labeling. This was representatively shown by PAGE analysis after the "click"-type cycloaddition with the fluorescent nile red azide. These results show that the 2'-position as one of the most important modification sites in oligonucleotides is now accessible not only for synthetic, but also for enzymatic oligonucleotide preparation. Topics: Adenosine Triphosphate; Click Chemistry; DNA; Fluorescent Dyes; Guanosine Triphosphate; Oligonucleotides; Oxazines; Uridine Triphosphate	2013

Article

Year

Synthesis of 2'-O-propargyl nucleoside triphosphates for enzymatic oligonucleotide preparation and "click" modification of DNA with Nile red as fluorescent probe.

Bioconjugate chemistry, 2013, Mar-20, Volume: 24, Issue:3

Uridine, adenosine, guanosine, and cytidine that carry a propargyl group attached to the 2'-oxygen were converted efficiently to the corresponding nucleoside triphosphates (pNTPs). Primer extension experiments revealed that pUTP, pATP, and pGTP can be successfully incorporated in oligonucleotides in the so-called 9°N and Therminator DNA polymerases. Most importantly, the ethynyl group as single 2'-modification of the enzymatically prepared oligonucleotides can be applied for postsynthetic labeling. This was representatively shown by PAGE analysis after the "click"-type cycloaddition with the fluorescent nile red azide. These results show that the 2'-position as one of the most important modification sites in oligonucleotides is now accessible not only for synthetic, but also for enzymatic oligonucleotide preparation.

Topics: Adenosine Triphosphate; Click Chemistry; DNA; Fluorescent Dyes; Guanosine Triphosphate; Oligonucleotides; Oxazines; Uridine Triphosphate

2013