guanosine-triphosphate and monodansylcadaverine

Actin from human blood platelets or rabbit skeletal muscle can serve as substrate for factor XIIIa. The latter catalyzes the incorporation of 1.5-2 mol monodansylcadaverine/mol rabbit actin and 0.5 mol/mol platelet actin. Highly cross-linked platelet and muscle actin polymers form in the absence of added amines, indicating the presence of both acceptor and donor sites. As expected, the cross-link was found to be a gamma-glutamyl-epsilon-lysine bone, with an average of 0.3-0.4 mol dipeptide/mol platelet actin. Both cross-linking and amine incorporation are prevented by ATP, ADP, GTP, CTP, but not by AMP and cyclic AMP. These nucleotides may have important regulatory role in muscle and non-muscle systems.

Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Blood Platelets; Cadaverine; Cytidine Triphosphate; Dansyl Compounds; Factor XIII; Guanosine Triphosphate; Humans; Macromolecular Substances; Muscles; Peptide Fragments; Rabbits