guanosine-triphosphate has been researched along with calpastatin* in 1 studies
1 other study(ies) available for guanosine-triphosphate and calpastatin
| Article | Year |
|---|---|
Calpastatin and nucleotides stabilize cardiac calcium channel activity in excised patches.
The activity of single L-type Ca2+ channels is rapidly lost (run-down) when contact between the membrane and cytosol is interrupted. We have now achieved the stabilization of cardiac Ca2+ channel activity of guinea-pig ventricular myocytes by using either cytosol or defined components added to excised patches. The endogenous protease inhibitor, calpastatin, together with nucleotides, ATP + GTP, was found to prevent run-down as effectively as cardiac cytosolic solution. These results suggest the involvement of proteolysis by calpain in run-down of channel activity and enable the study of cardiac Ca2+ channel regulation with free access to both sides of the membrane. Topics: 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; Adenosine Triphosphate; Animals; Calcium Channels; Calcium-Binding Proteins; Cysteine Proteinase Inhibitors; Electric Conductivity; Guanosine Triphosphate; Guinea Pigs; In Vitro Techniques; Myocardium | 1991 |