guanosine-triphosphate and 9-(2-hydroxy-3-nonyl)adenine

The pharmacokinetics of erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) inhibition of adenosine deaminase (ADA) was measured in vivo in CBA mice. The in vivo assay utilized injection of 10-100 nmoles [2-3H]adenosine and measurement of blood 3H2O 20 min later. A single oral dose of EHNA (50 mg/kg) totally inhibited ADA for 4 hr and caused a large increase in conversion of [2-3H]adenosine to [2-3H]ATP. EHNA (3 mg/kg) decreased deamination by 50% for 2-6 hr, depending on the dose of adenosine used. Mice dosed with EHNA (100 mg/kg) once daily for 7 days showed the same ADA recovery rate as mice dosed only once. High single oral doses of EHNA had no effect on blood ATP and GTP pools.

Topics: Adenine; Adenosine Deaminase Inhibitors; Adenosine Triphosphate; Animals; Dose-Response Relationship, Drug; Erythrocytes; Guanosine Triphosphate; Kinetics; Mice; Mice, Inbred CBA; Nucleoside Deaminases