guanosine-triphosphate and 1-6-bis(cyclohexyloximinocarbonyl)hexane

guanosine-triphosphate has been researched along with 1-6-bis(cyclohexyloximinocarbonyl)hexane* in 1 studies

Other Studies

1 other study(ies) available for guanosine-triphosphate and 1-6-bis(cyclohexyloximinocarbonyl)hexane

Article	Year
A G-protein mediates secretagogue-induced gap junctional channel closure in pancreatic acinar cells. FEBS letters, 1989, Dec-04, Volume: 258, Issue:2 Using the double whole-cell patch-clamp technique, we determined that dialysis of cell pairs by GTP[S] potentiated electrical uncoupling induced by extracellular addition of carbamylcholine (CCh). An inhibitor of diglyceride lipase, RHC 80267, further potentiated CCh/GTP[S]-induced junctional channel closure, probably by accumulation of diacylglycerol. Moreover, the protein kinase C inhibitor polymyxin B completely blocked uncoupling elicited by CCh/GTP[S]. These results provide the first evidence suggesting that gap junction channel closure by cholinergic stimulation is mediated by a G-protein, which acts by increasing phosphatidylinositol biphosphate breakdown and protein kinase C activity. Topics: Animals; Carbachol; Cyclohexanones; Electric Conductivity; GTP-Binding Proteins; Guanosine 5'-O-(3-Thiotriphosphate); Guanosine Triphosphate; In Vitro Techniques; Intercellular Junctions; Ion Channels; Kinetics; Lipase; Male; Mice; Mice, Inbred Strains; Pancreas; Thionucleotides	1989

Article

Year

A G-protein mediates secretagogue-induced gap junctional channel closure in pancreatic acinar cells.

FEBS letters, 1989, Dec-04, Volume: 258, Issue:2

Using the double whole-cell patch-clamp technique, we determined that dialysis of cell pairs by GTP[S] potentiated electrical uncoupling induced by extracellular addition of carbamylcholine (CCh). An inhibitor of diglyceride lipase, RHC 80267, further potentiated CCh/GTP[S]-induced junctional channel closure, probably by accumulation of diacylglycerol. Moreover, the protein kinase C inhibitor polymyxin B completely blocked uncoupling elicited by CCh/GTP[S]. These results provide the first evidence suggesting that gap junction channel closure by cholinergic stimulation is mediated by a G-protein, which acts by increasing phosphatidylinositol biphosphate breakdown and protein kinase C activity.

Topics: Animals; Carbachol; Cyclohexanones; Electric Conductivity; GTP-Binding Proteins; Guanosine 5'-O-(3-Thiotriphosphate); Guanosine Triphosphate; In Vitro Techniques; Intercellular Junctions; Ion Channels; Kinetics; Lipase; Male; Mice; Mice, Inbred Strains; Pancreas; Thionucleotides

1989