guanosine-tetraphosphate has been researched along with adenosine-5--triphosphate-3--diphosphate* in 2 studies
2 other study(ies) available for guanosine-tetraphosphate and adenosine-5--triphosphate-3--diphosphate
| Article | Year |
|---|---|
Effect of adenosine-5'-triphosphate-3'-diphosphate and related nucleoside polyphosphates on the spore germination of Streptomyces galilaeus.
Exogenous addition of adenosine- and guanosine 5'-(di- and tri) phosphate 3'-diphosphate (pppApp, ppApp, pppGpp and ppGpp) at the concentration of 0.5 mM inhibits spore germination of Streptomyces galilaeus ATCC 31133. This reversible inhibitory effect appeared to be at the transcriptional level, and also depends on the phase of spore germination; pppApp inhibited more strongly RNA synthesis in the period of the germ tube emergence than the early stage of germination. No inhibitory effect was observed with normal purine and pyrimidine nucleosides, nucleotides, pApp, pGpp, cyclic AMP and pyrophosphoric acid at the concentration of 0.1 - 1.0 mM. Topics: Adenine Nucleotides; DNA, Bacterial; Guanine Nucleotides; Guanosine Pentaphosphate; Guanosine Tetraphosphate; Nucleosides; Purine Nucleotides; Pyrimidine Nucleotides; RNA, Bacterial; Spores, Bacterial; Streptomyces | 1980 |
A radioimmunoassay for guanosine-5'-diphosphate-3'-diphosphate and adenosine-5'-triphosphate-3'-diphosphate.
A radioimmunoassay for guanosine-5'-diphosphate-3'-diphosphate (ppGpp) and adenosine-5'-triphosphate-3'-diphosphate (pppApp) has been developed. The assay method is based on competition of an unlabeled highly phosphorylated nucleotide with 3H-labeled highly phosphorylated nucleotide for binding sites on a specific antibody. Antibodies to ppGpp and pppApp were obtained by immunizing rabbits with the antigen prepared by conjugating ppGpp with human serum albumin using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, and with the antigen prepared by conjugating 8-(6-aminohexyl)amino-adenosine-5'-triphosphate-3'-diphosphate with human serum albumin using glutaraldehyde, respectively. Antibody-bound 3H-labeled highly phosphorylated nucleotides were separated from the free 3H-labeled highly phosphorylated nucleotides by selective adsorption on dextran-coated charcoal. Displacement plots were linear over a concentration range of 5-1,000 pmol/assay tube in a log-probit percentage plot. Application of this method to biological systems offers improved accuracy and convenience compared with the previous 32PO4-labeling technique. Topics: Adenine Nucleotides; Bacillus subtilis; Escherichia coli; Ethyldimethylaminopropyl Carbodiimide; Glutaral; Guanine Nucleotides; Guanosine Tetraphosphate; Humans; Radioimmunoassay; Serum Albumin; Streptomyces | 1980 |