guanosine-tetraphosphate has been researched along with 1-2-benzisothiazoline-3-one* in 1 studies
1 other study(ies) available for guanosine-tetraphosphate and 1-2-benzisothiazoline-3-one
Article | Year |
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ppGpp accumulation in Pseudomonas aeruginosa and Pseudomonas fluorescens subjected to nutrient limitation and biocide exposure.
The effect of a commonly used biocide, 1,2-benzisothiazolin-3-one (BIT) on ppGpp accumulation in the pathogen, Pseudomonas aeruginosa PAO1, and an environmental isolate, Ps. fluorescens, was examined. It is concluded that BIT is able to induce a stringent response in Ps. aeruginosa and Ps. fluorescens, determined by the rapid accumulation of ppGpp following addition of BIT to exponentially-growing cells. Western blot analysis of whole-cell extracts with anti-RelA antibody demonstrated that both species contain a RelA homologue. This is the first report of a RelA-like protein in pseudomonads. Topics: Blotting, Western; Culture Media; Guanosine Tetraphosphate; Microbial Sensitivity Tests; Nitrogen; Pseudomonas aeruginosa; Pseudomonas fluorescens; Reproducibility of Results; Thiazoles | 1999 |